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Compositional analysis of dairy fats...

Cruz Hernandez, Cristina.

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Compositional analysis of dairy fats, phospholipids and positional distribution on TAG from cows fed fish meal.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Compositional analysis of dairy fats, phospholipids and positional distribution on TAG from cows fed fish meal./
作者:	Cruz Hernandez, Cristina.
面頁冊數:	190 p.
附註:	Source: Dissertation Abstracts International, Volume: 65-02, Section: B, page: 0493.
Contained By:	Dissertation Abstracts International65-02B.
標題:	Agriculture, Food Science and Technology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ89250
ISBN:	0612892506

Compositional analysis of dairy fats, phospholipids and positional distribution on TAG from cows fed fish meal.
Cruz Hernandez, Cristina.

Compositional analysis of dairy fats, phospholipids and positional distribution on TAG from cows fed fish meal. - 190 p.

Source: Dissertation Abstracts International, Volume: 65-02, Section: B, page: 0493.

Thesis (Ph.D.)--University of Guelph (Canada), 2004.

DHA enriched milk fat samples obtained at the University of Guelph from cows fed fish meal (FM) were characterized and compared with milk from cows fed corn/soy silage. The total FA was analyzed by GC equipped with 100m CP Sill 88 capillary column by which more than 100 FA were identified. Ag +-TLC combined with GC was used to analyze all possible positional cis and trans monoenes. GC and Ag+-HPLC was used to resolve all CLA isomers. 9c11t, 18:2 was the major CLA isomer found in both diets. The other major CLA isomers were 9t11c and 7t9c; the former was identified by GC, and the latter by Ag+-HPLC.

ISBN: 0612892506Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Compositional analysis of dairy fats, phospholipids and positional distribution on TAG from cows fed fish meal.
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245 1 0 $a Compositional analysis of dairy fats, phospholipids and positional distribution on TAG from cows fed fish meal.
300 $a 190 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-02, Section: B, page: 0493.
500 $a Advisers: John K. G. Kramer; Art R. Hill.
502 $a Thesis (Ph.D.)--University of Guelph (Canada), 2004.
520 $a DHA enriched milk fat samples obtained at the University of Guelph from cows fed fish meal (FM) were characterized and compared with milk from cows fed corn/soy silage. The total FA was analyzed by GC equipped with 100m CP Sill 88 capillary column by which more than 100 FA were identified. Ag +-TLC combined with GC was used to analyze all possible positional cis and trans monoenes. GC and Ag+-HPLC was used to resolve all CLA isomers. 9c11t, 18:2 was the major CLA isomer found in both diets. The other major CLA isomers were 9t11c and 7t9c; the former was identified by GC, and the latter by Ag+-HPLC.
520 $a The TAG and PL were separated using Supelco LC-Sil columns. PL were analyzed and distributed into three major classes: PC, PE and SM. SM was the most saturated PL fraction (ca. 70%). There was an increase in the degree of unsaturation of the analyzed PL in the following order PE > PC > SM. The CLA content in the major PL was lower than in TAG.
520 $a Positional analysis of TAG was performed enzymaticaly and using Grignard reactions. The MAG were isolated on boric acid impregnated TLC plates and analyzed as their butyl esters by GC, which was confirmed by GC-MS. The distribution of short chain-FA was found in both sn-1,3 and sn -2 positions. For FM samples, C12:0, C16:0 and C18:0 FA were predominantly at sn-1,3 position.
520 $a The inclusion of FM into diets of dairy cows resulted in an increase of DHA (0.04 to 0.17%). Increases were also observed for 9c11t-CLA (0.39% to 0.66%), 7t9c-CLA (0.05% to 0.26%), and 9t11c-CLA isomers (0.05 to 0.13%). 10t-18:1 increased (0.57% to 4.58%) and 11t-18:1 decreased (1.16 to 0.96%).
590 $a School code: 0081.
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710 2 0 $a University of Guelph (Canada). $3 1018650
773 0 $t Dissertation Abstracts International $g 65-02B.
790 1 0 $a Kramer, John K. G., $e advisor
790 1 0 $a Hill, Art R., $e advisor
790 $a 0081
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ89250