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Two- and three-dimensional image ana...

Yamaguchi, Yoshinori.

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Two- and three-dimensional image analysis in bioanalytical measurement systems.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Two- and three-dimensional image analysis in bioanalytical measurement systems./
作者:	Yamaguchi, Yoshinori.
面頁冊數:	231 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4328.
Contained By:	Dissertation Abstracts International64-09B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3106192

Two- and three-dimensional image analysis in bioanalytical measurement systems.
Yamaguchi, Yoshinori.

Two- and three-dimensional image analysis in bioanalytical measurement systems. - 231 p.

Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4328.

Thesis (Ph.D.)--University of Michigan, 2003.

Statistics of DNA single molecule dynamics in DC electrophoresis systems were used to predict ensemble electrophoretic migration times and overall band shapes. Migration times are predicted adequately and the asymmetric band shapes observed in electrophoresis are mirrored in asymmetric migration time distributions of single molecules. The proper scaling relation is not known so it is not been possible to establish whether or not the data accurately predicts bandwidth from single DNA molecule dynamics. Similar successes and limitations are observed for field inversion electrophoresis. Sample size studies show that the range of possibilities for DNA motions is limited and much of it is explored rapidly.Subjects--Topical Terms:

586156
Chemistry, Analytical.

Two- and three-dimensional image analysis in bioanalytical measurement systems.
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245 1 0 $a Two- and three-dimensional image analysis in bioanalytical measurement systems.
300 $a 231 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4328.
500 $a Chair: Michael D. Morris.
502 $a Thesis (Ph.D.)--University of Michigan, 2003.
520 $a Statistics of DNA single molecule dynamics in DC electrophoresis systems were used to predict ensemble electrophoretic migration times and overall band shapes. Migration times are predicted adequately and the asymmetric band shapes observed in electrophoresis are mirrored in asymmetric migration time distributions of single molecules. The proper scaling relation is not known so it is not been possible to establish whether or not the data accurately predicts bandwidth from single DNA molecule dynamics. Similar successes and limitations are observed for field inversion electrophoresis. Sample size studies show that the range of possibilities for DNA motions is limited and much of it is explored rapidly.
520 $a The first three-dimensional (3-D) description of DNA electrophoretic migration is reported. The assumption of uniform probability of out of plane orientations, frequently made for DNA migration theory, has been verified. However the use of apparent two dimensional (2-D) opening angles underestimates the true behavior. An extension of the Oana/Doi ellipsoidal approximation to DNA dimensions is used for quantitative descriptions. Although several limitations of the 3-D ellipsoid approximation are pointed out, it is sufficient for DNA migration image analysis.
520 $a Scanning electron microscopy (SEM) and extended focus (EF) light microscopy were used for acquiring surface images of electrochemical surface enhanced Raman spectroscopy (SERS) substrates. Self-similarity was found from 30 mum to 100 nm and was used to define an optimum fractal dimension for SERS activity, approximately 1.7. The activity thresholds found by both SEM and EF light microscopy are similar. Therefore, EF microscopy can be used to assess the quality of an electrode surface before it is irrevocably committed to an experiment.
590 $a School code: 0127.
650 4 $a Chemistry, Analytical. $3 586156
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710 2 0 $a University of Michigan. $3 777416
773 0 $t Dissertation Abstracts International $g 64-09B.
790 1 0 $a Morris, Michael D., $e advisor
790 $a 0127
791 $a Ph.D.
792 $a 2003
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