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Creating genetic engineering tools f...

Anderson, Robert Clayton, III.

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Creating genetic engineering tools for investigating Bacillus anthracis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Creating genetic engineering tools for investigating Bacillus anthracis./
Author:	Anderson, Robert Clayton, III.
Description:	92 p.
Notes:	Source: Masters Abstracts International, Volume: 42-04, page: 1207.
Contained By:	Masters Abstracts International42-04.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1418466

Creating genetic engineering tools for investigating Bacillus anthracis.
Anderson, Robert Clayton, III.

Creating genetic engineering tools for investigating Bacillus anthracis. - 92 p.

Source: Masters Abstracts International, Volume: 42-04, page: 1207.

Thesis (M.S.)--East Tennessee State University, 2003.

Bacillus anthracis is a Gram positive, spore forming, non-motile, rod shaped, soil bacterium, and is endemic worldwide. Currently, the biology of B. anthracis is poorly understood. B. anthracis is one of many biological weapons used today. A -/- mutant strain of B. anthracis that lacks the pathogenic plasmids was created by serial culture at 42°C. Key DNA replication genes were identified by homology as targets. The dnaB gene, essential for B. subtilis initiation of DNA replication, was my focus. A vector system was created by polymerase chain reaction (PCR) with the pMUTIN4 integration vector and the promoter region of dnaB to study the genetics of B. anthracis. An electro-transformation system was formulated to knock-out the -/- B. anthracis dnaB gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of B. anthracis. We also have formulated an electro-transformation system and vector system for use in B. anthracis .Subjects--Topical Terms:

1017730
Biology, Genetics.

Creating genetic engineering tools for investigating Bacillus anthracis.
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035 $a (UnM)AAI1418466
035 $a AAI1418466
040 $a UnM $c UnM
100 1 $a Anderson, Robert Clayton, III. $3 1948746
245 1 0 $a Creating genetic engineering tools for investigating Bacillus anthracis.
300 $a 92 p.
500 $a Source: Masters Abstracts International, Volume: 42-04, page: 1207.
500 $a Chair: John J. Laffan.
502 $a Thesis (M.S.)--East Tennessee State University, 2003.
520 $a Bacillus anthracis is a Gram positive, spore forming, non-motile, rod shaped, soil bacterium, and is endemic worldwide. Currently, the biology of B. anthracis is poorly understood. B. anthracis is one of many biological weapons used today. A -/- mutant strain of B. anthracis that lacks the pathogenic plasmids was created by serial culture at 42°C. Key DNA replication genes were identified by homology as targets. The dnaB gene, essential for B. subtilis initiation of DNA replication, was my focus. A vector system was created by polymerase chain reaction (PCR) with the pMUTIN4 integration vector and the promoter region of dnaB to study the genetics of B. anthracis. An electro-transformation system was formulated to knock-out the -/- B. anthracis dnaB gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of B. anthracis. We also have formulated an electro-transformation system and vector system for use in B. anthracis .
590 $a School code: 0069.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0369
690 $a 0410
710 2 0 $a East Tennessee State University. $3 1017802
773 0 $t Masters Abstracts International $g 42-04.
790 1 0 $a Laffan, John J., $e advisor
790 $a 0069
791 $a M.S.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1418466