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Soil microcosms as environmental res...

Hynes, Samielle.

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Soil microcosms as environmental research tools for the study of microorganism gene transfer in soil environments.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Soil microcosms as environmental research tools for the study of microorganism gene transfer in soil environments./
Author:	Hynes, Samielle.
Description:	135 p.
Notes:	Source: Masters Abstracts International, Volume: 42-01, page: 0163.
Contained By:	Masters Abstracts International42-01.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ79812
ISBN:	0612798127

Soil microcosms as environmental research tools for the study of microorganism gene transfer in soil environments.
Hynes, Samielle.

Soil microcosms as environmental research tools for the study of microorganism gene transfer in soil environments. - 135 p.

Source: Masters Abstracts International, Volume: 42-01, page: 0163.

Thesis (M.Sc.)--Carleton University (Canada), 2003.

The evaluation of risk involved in the release of genetically modified micro-organisms (GMO) to the environment has environmental and economic implications. In this project we released a strain of Pseudomonas putida, genetically modified to contain a self-transmissible atrazine-degradation plasmid, in the field and in contained soil microcosms. We also monitored the persistence of atrazine degradation in the soil following release. Atrazine-degrading bacteria thrived during the first 48 hr after release and then rapidly declined in numbers. To follow the horizontal transfer of atrazine degradation genes, transconjugant bacteria were isolated and identified. Of the 150 potential transconjugants isolated 10 were chosen for further characterization. Of these, 8 were Pseudomonad spp. isolated directly from experimental trials, and 2 were Arthrobacter from subsequent enrichment experiments. This study demonstrated that horizontal gene transfer can occur in the soil and is therefore an important consideration for any environmental release of a GMO.

ISBN: 0612798127Subjects--Topical Terms:

1017730
Biology, Genetics.

Soil microcosms as environmental research tools for the study of microorganism gene transfer in soil environments.
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245 1 0 $a Soil microcosms as environmental research tools for the study of microorganism gene transfer in soil environments.
300 $a 135 p.
500 $a Source: Masters Abstracts International, Volume: 42-01, page: 0163.
500 $a Adviser: Sharon Regan.
502 $a Thesis (M.Sc.)--Carleton University (Canada), 2003.
520 $a The evaluation of risk involved in the release of genetically modified micro-organisms (GMO) to the environment has environmental and economic implications. In this project we released a strain of Pseudomonas putida, genetically modified to contain a self-transmissible atrazine-degradation plasmid, in the field and in contained soil microcosms. We also monitored the persistence of atrazine degradation in the soil following release. Atrazine-degrading bacteria thrived during the first 48 hr after release and then rapidly declined in numbers. To follow the horizontal transfer of atrazine degradation genes, transconjugant bacteria were isolated and identified. Of the 150 potential transconjugants isolated 10 were chosen for further characterization. Of these, 8 were Pseudomonad spp. isolated directly from experimental trials, and 2 were Arthrobacter from subsequent enrichment experiments. This study demonstrated that horizontal gene transfer can occur in the soil and is therefore an important consideration for any environmental release of a GMO.
590 $a School code: 0040.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Environmental Sciences. $3 676987
650 4 $a Biology, Microbiology. $3 1017734
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773 0 $t Masters Abstracts International $g 42-01.
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