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Chemotaxis and signal transduction i...

Hawkins, Andrew Charles.

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Chemotaxis and signal transduction in two proteobacteria: Pseudomonas aeruginosa and Ralstonia eutropha.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Chemotaxis and signal transduction in two proteobacteria: Pseudomonas aeruginosa and Ralstonia eutropha./
Author:	Hawkins, Andrew Charles.
Description:	151 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 5916.
Contained By:	Dissertation Abstracts International64-12B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3114358

Chemotaxis and signal transduction in two proteobacteria: Pseudomonas aeruginosa and Ralstonia eutropha.
Hawkins, Andrew Charles.

Chemotaxis and signal transduction in two proteobacteria: Pseudomonas aeruginosa and Ralstonia eutropha. - 151 p.

Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 5916.

Thesis (Ph.D.)--The University of Iowa, 2003.

Pseudomonas aeruginosa has five clusters of Escherichia coli-like chemotaxis genes. Clusters I and V are required for swimming motility chemotaxis. Cluster III is involved in cell autolysis and autoaggregation. Cluster IV is involved in twitching motility. Cluster II, an alternate set of eight E. coli-like chemotaxis genes, had no known function. Mutations were constructed in seven cluster II genes: mcpA, cheY2, cheA2, cheW2 , mcpB, cheR2, and cheB2 . An extensive analysis of these mutants indicates that cluster II genes likely play only a minor role in P. aeruginosa chemotaxis. Results indicate that cluster II genes are optimally expressed during the stationary phase of growth. The expression of cluster II genes requires the stationary phase sigma factor RpoS. Cluster II genes are positively regulated 3- to 4-fold by the quorum sensing system of P. aeruginosa. Examination of stationary phase P. aeruginosa cells has indicated that cluster II genes affect cell surface properties of P. aeruginosa and may be involved in biofilm formation.Subjects--Topical Terms:

1017734
Biology, Microbiology.

Chemotaxis and signal transduction in two proteobacteria: Pseudomonas aeruginosa and Ralstonia eutropha.
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035 $a (UnM)AAI3114358
035 $a AAI3114358
040 $a UnM $c UnM
100 1 $a Hawkins, Andrew Charles. $3 1948673
245 1 0 $a Chemotaxis and signal transduction in two proteobacteria: Pseudomonas aeruginosa and Ralstonia eutropha.
300 $a 151 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 5916.
500 $a Supervisor: Caroline S. Harwood.
502 $a Thesis (Ph.D.)--The University of Iowa, 2003.
520 $a Pseudomonas aeruginosa has five clusters of Escherichia coli-like chemotaxis genes. Clusters I and V are required for swimming motility chemotaxis. Cluster III is involved in cell autolysis and autoaggregation. Cluster IV is involved in twitching motility. Cluster II, an alternate set of eight E. coli-like chemotaxis genes, had no known function. Mutations were constructed in seven cluster II genes: mcpA, cheY2, cheA2, cheW2 , mcpB, cheR2, and cheB2 . An extensive analysis of these mutants indicates that cluster II genes likely play only a minor role in P. aeruginosa chemotaxis. Results indicate that cluster II genes are optimally expressed during the stationary phase of growth. The expression of cluster II genes requires the stationary phase sigma factor RpoS. Cluster II genes are positively regulated 3- to 4-fold by the quorum sensing system of P. aeruginosa. Examination of stationary phase P. aeruginosa cells has indicated that cluster II genes affect cell surface properties of P. aeruginosa and may be involved in biofilm formation.
520 $a TfdK, from Ralstonia eutropha, is a member of the Major Facilitator Superfamily (MFS) of membrane transport proteins. TfdK actively transports 2,4-dichlorophenoxyacetate (2,4-D), a widely used herbicide. A topological model of TfdK embedded in the membrane was developed using a computer algorithm. TfdK is not similar to chemotaxis proteins from E. coli . TfdK has high amino acid sequence identity to PcaK from Pseudomonas putida, a MFS transporter involved in transport of and chemotaxis to 4-hydroxybenzoate. The similarity of TfdK to PcaK lead to the hypothesis that R. eutropha is chemotactically attracted to 2,4-D and that TfdK is required for the chemotactic response. Results indicate that R. eutropha is chemotactically attracted to 2,4-D and that this is an inducible response. Results also show that TfdK, encoded on the catabolic plasmid pJP4, is required for this response. This is the first report of chemotaxis to 2,4-D. TfdK is the second MFS protein to be implicated in chemotaxis as well as transport. The mechanism for PcaK- or TfdK-mediated chemotaxis is still unknown.
590 $a School code: 0096.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0410
690 $a 0307
710 2 0 $a The University of Iowa. $3 1017439
773 0 $t Dissertation Abstracts International $g 64-12B.
790 1 0 $a Harwood, Caroline S., $e advisor
790 $a 0096
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3114358