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Biomolecular recognition: From cyan...

Cao, Rong.

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Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes./
作者:	Cao, Rong.
面頁冊數:	234 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4330.
Contained By:	Dissertation Abstracts International64-09B.
標題:	Chemistry, Biochemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3104357

Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes.
Cao, Rong.

Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes. - 234 p.

Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4330.

Thesis (Ph.D.)--Carnegie Mellon University, 2003.

Biomolecular recognition is very important in life sciences in that almost all biological processes are controlled by the noncovalent association of two or more molecules. The assembly of novel biomaterials such as DNA and proteins based on biomolecular recognition is the main goal of both biotechnology and nanotechnology. This thesis work involved the biomolecular recognition motif from organic dyes, nucleic acids to proteins and enzymes.Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes.
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245 1 0 $a Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes.
300 $a 234 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4330.
500 $a Adviser: Bruce Armitage.
502 $a Thesis (Ph.D.)--Carnegie Mellon University, 2003.
520 $a Biomolecular recognition is very important in life sciences in that almost all biological processes are controlled by the noncovalent association of two or more molecules. The assembly of novel biomaterials such as DNA and proteins based on biomolecular recognition is the main goal of both biotechnology and nanotechnology. This thesis work involved the biomolecular recognition motif from organic dyes, nucleic acids to proteins and enzymes.
520 $a An introduction to DNA-small molecule interactions in <italic>CHAPTER I</italic> outlines the typical binding modes between them and gives a variety of examples of groove binders including major and minor groove binding ligands and intercalators. Sequence-dependent binding modes are introduced.
520 $a In <italic>CHAPTER II</italic>, a combination of spectroscopic (UV-vis, CD, fluorescence) and hydrodynamic (viscometric) methods were used to study the interaction of a symmetrical cyanine dye <bold>DiSC<sub>3+</sub>(5)</bold> with a series of duplex and triplex sequences. The binding modes were assigned based on the criteria described in <italic>CHAPTER I</italic>. These studies demonstrate the diversity of DNA binding modes to a given ligand structure.
520 $a In <italic>CHAPTER III</italic>, the interaction of methylphosphonate DNA (MP DNA is an analogue of DNA with the neutralized backbone) with cyanine dye and Hoechst 33258 were investigated by using UV melting curves, CD and fluorescence measurements. The results were compared with those obtained from natural DNA. It was found that the binding of cyanine dye with MP DNA duplex is inhibited, while the MP DNA linkage actually promotes the binding of Hoechst 33258 to MP DNA duplex.
520 $a A further introduction to oligonucleotide-directed biomolecular self-assembly was given in <italic>CHAPTER IV</italic>. This introduction chapter gives the description of biomolecular self-assembly into a gel network based on DNA nanotechnology, protein-ligand interaction, and PNA/DNA hybridization (PNA is a DNA mimic whose properties are also described). The properties of gel systems, especially thermoreversible gels, are also described. One of the most important applications of thermoreversible, immobilization of enzymes, is further introduced.
520 $a <italic>CHAPTER V</italic> deals with the design, synthesis and characterization of a thermoreversible biopolymer microgel on the basis of DNA TWJ, avidin-biotin interaction, and PNA/DNA hybridization. The system has well defined functionality as well as fine control over the gelation temperature to produce micron-sized particles. UV-melting transition measurement, fluorescence spectra, and laser light scattering were used to study the thermoreversible assembly process and potential future design was proposed.
520 $a <italic>CHAPTER VI</italic> further explores the ability of the above microgel to entrap avidin-β-galactosidase enzyme with retention of enzyme activity, good reusability, and ease of separation of the enzyme from the solution.
590 $a School code: 0041.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Organic. $3 516206
650 4 $a Biology, Molecular. $3 1017719
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690 $a 0490
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710 2 0 $a Carnegie Mellon University. $3 1018096
773 0 $t Dissertation Abstracts International $g 64-09B.
790 1 0 $a Armitage, Bruce, $e advisor
790 $a 0041
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3104357