東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Biomolecular recognition: From cyan...

Cao, Rong.

Linked to FindBook

Google Book

Amazon

博客來

Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes./
Author:	Cao, Rong.
Description:	234 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4330.
Contained By:	Dissertation Abstracts International64-09B.
Subject:	Chemistry, Biochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3104357

Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes.
Cao, Rong.

Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes. - 234 p.

Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4330.

Thesis (Ph.D.)--Carnegie Mellon University, 2003.

Biomolecular recognition is very important in life sciences in that almost all biological processes are controlled by the noncovalent association of two or more molecules. The assembly of novel biomaterials such as DNA and proteins based on biomolecular recognition is the main goal of both biotechnology and nanotechnology. This thesis work involved the biomolecular recognition motif from organic dyes, nucleic acids to proteins and enzymes.Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes.
LDR:03964nmm 2200349 4500 001 1859397
005 20041014084350.5
008 130614s2003 eng d
035 $a (UnM)AAI3104357
035 $a AAI3104357
040 $a UnM $c UnM
100 1 $a Cao, Rong. $3 1947056
245 1 0 $a Biomolecular recognition: From cyanine dye-DNA complexes to recyclable enzymes.
300 $a 234 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-09, Section: B, page: 4330.
500 $a Adviser: Bruce Armitage.
502 $a Thesis (Ph.D.)--Carnegie Mellon University, 2003.
520 $a Biomolecular recognition is very important in life sciences in that almost all biological processes are controlled by the noncovalent association of two or more molecules. The assembly of novel biomaterials such as DNA and proteins based on biomolecular recognition is the main goal of both biotechnology and nanotechnology. This thesis work involved the biomolecular recognition motif from organic dyes, nucleic acids to proteins and enzymes.
520 $a An introduction to DNA-small molecule interactions in <italic>CHAPTER I</italic> outlines the typical binding modes between them and gives a variety of examples of groove binders including major and minor groove binding ligands and intercalators. Sequence-dependent binding modes are introduced.
520 $a In <italic>CHAPTER II</italic>, a combination of spectroscopic (UV-vis, CD, fluorescence) and hydrodynamic (viscometric) methods were used to study the interaction of a symmetrical cyanine dye <bold>DiSC<sub>3+</sub>(5)</bold> with a series of duplex and triplex sequences. The binding modes were assigned based on the criteria described in <italic>CHAPTER I</italic>. These studies demonstrate the diversity of DNA binding modes to a given ligand structure.
520 $a In <italic>CHAPTER III</italic>, the interaction of methylphosphonate DNA (MP DNA is an analogue of DNA with the neutralized backbone) with cyanine dye and Hoechst 33258 were investigated by using UV melting curves, CD and fluorescence measurements. The results were compared with those obtained from natural DNA. It was found that the binding of cyanine dye with MP DNA duplex is inhibited, while the MP DNA linkage actually promotes the binding of Hoechst 33258 to MP DNA duplex.
520 $a A further introduction to oligonucleotide-directed biomolecular self-assembly was given in <italic>CHAPTER IV</italic>. This introduction chapter gives the description of biomolecular self-assembly into a gel network based on DNA nanotechnology, protein-ligand interaction, and PNA/DNA hybridization (PNA is a DNA mimic whose properties are also described). The properties of gel systems, especially thermoreversible gels, are also described. One of the most important applications of thermoreversible, immobilization of enzymes, is further introduced.
520 $a <italic>CHAPTER V</italic> deals with the design, synthesis and characterization of a thermoreversible biopolymer microgel on the basis of DNA TWJ, avidin-biotin interaction, and PNA/DNA hybridization. The system has well defined functionality as well as fine control over the gelation temperature to produce micron-sized particles. UV-melting transition measurement, fluorescence spectra, and laser light scattering were used to study the thermoreversible assembly process and potential future design was proposed.
520 $a <italic>CHAPTER VI</italic> further explores the ability of the above microgel to entrap avidin-β-galactosidase enzyme with retention of enzyme activity, good reusability, and ease of separation of the enzyme from the solution.
590 $a School code: 0041.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Organic. $3 516206
650 4 $a Biology, Molecular. $3 1017719
690 $a 0487
690 $a 0490
690 $a 0307
710 2 0 $a Carnegie Mellon University. $3 1018096
773 0 $t Dissertation Abstracts International $g 64-09B.
790 1 0 $a Armitage, Bruce, $e advisor
790 $a 0041
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3104357