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Cloning and sequencing of glucosyltr...

Sibhatu, Mebrahtu Berhane.

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Cloning and sequencing of glucosyltransferase (GT) genes from grapefruit (Citrus paradisi) seedlings: Searching for 7-O-GT.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Cloning and sequencing of glucosyltransferase (GT) genes from grapefruit (Citrus paradisi) seedlings: Searching for 7-O-GT./
Author:	Sibhatu, Mebrahtu Berhane.
Description:	145 p.
Notes:	Source: Masters Abstracts International, Volume: 42-01, page: 0237.
Contained By:	Masters Abstracts International42-01.
Subject:	Chemistry, Biochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1415342

Cloning and sequencing of glucosyltransferase (GT) genes from grapefruit (Citrus paradisi) seedlings: Searching for 7-O-GT.
Sibhatu, Mebrahtu Berhane.

Cloning and sequencing of glucosyltransferase (GT) genes from grapefruit (Citrus paradisi) seedlings: Searching for 7-O-GT. - 145 p.

Source: Masters Abstracts International, Volume: 42-01, page: 0237.

Thesis (M.S.)--East Tennessee State University, 2003.

Flavonoids play crucial roles in plant life cycles and in human welfare. Grapefruit plants produce several glucosylated flavonoids and the glucosylation reactions are catalyzed by UDP-glucose: glucosyltransferases (GTs). Our objective was to use the SMART RACE RT-PCR strategy to obtain cDNA sequences of putative grapefruit flavonoid GTs. Gene specific primers were designed from the plant secondary product glucosyltransferase (PSPG) box and used to amplify 5<super> ′</super>GT clones. Clone-specific primers were designed from 5<super> ′</super>clones to amplify 3<super>′</super> GT clones. We obtained 5 5<super>′</super> clones and 1 3<super>′</super> clone as candidate GTs. We used 3 of these clones to predict 2 compiled GT sequences. Structural comparison of the putative GTs with functionally known GTs from other plants showed overall low pair-wise sequence identity (11–31%), but high identity (52.2–75%) within the PSPG box. We conclude that assignment of function from sequence information is not appropriate; assignment should depend on biochemical characterization of expressed GT enzymes.Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Cloning and sequencing of glucosyltransferase (GT) genes from grapefruit (Citrus paradisi) seedlings: Searching for 7-O-GT.
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100 1 $a Sibhatu, Mebrahtu Berhane. $3 1946084
245 1 0 $a Cloning and sequencing of glucosyltransferase (GT) genes from grapefruit (Citrus paradisi) seedlings: Searching for 7-O-GT.
300 $a 145 p.
500 $a Source: Masters Abstracts International, Volume: 42-01, page: 0237.
500 $a Co-Chairs: Cecilia A. McIntosh; Lee M. Pike.
502 $a Thesis (M.S.)--East Tennessee State University, 2003.
520 $a Flavonoids play crucial roles in plant life cycles and in human welfare. Grapefruit plants produce several glucosylated flavonoids and the glucosylation reactions are catalyzed by UDP-glucose: glucosyltransferases (GTs). Our objective was to use the SMART RACE RT-PCR strategy to obtain cDNA sequences of putative grapefruit flavonoid GTs. Gene specific primers were designed from the plant secondary product glucosyltransferase (PSPG) box and used to amplify 5<super> ′</super>GT clones. Clone-specific primers were designed from 5<super> ′</super>clones to amplify 3<super>′</super> GT clones. We obtained 5 5<super>′</super> clones and 1 3<super>′</super> clone as candidate GTs. We used 3 of these clones to predict 2 compiled GT sequences. Structural comparison of the putative GTs with functionally known GTs from other plants showed overall low pair-wise sequence identity (11–31%), but high identity (52.2–75%) within the PSPG box. We conclude that assignment of function from sequence information is not appropriate; assignment should depend on biochemical characterization of expressed GT enzymes.
590 $a School code: 0069.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Botany. $3 1017825
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690 $a 0307
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710 2 0 $a East Tennessee State University. $3 1017802
773 0 $t Masters Abstracts International $g 42-01.
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790 1 0 $a Pike, Lee M., $e advisor
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