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Analysis of promoter activity in Sta...

Ross, Christian Allen.

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Analysis of promoter activity in Staphylococcus aureus.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Analysis of promoter activity in Staphylococcus aureus./
Author:	Ross, Christian Allen.
Description:	170 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3110.
Contained By:	Dissertation Abstracts International64-07B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3098547

Analysis of promoter activity in Staphylococcus aureus.
Ross, Christian Allen.

Analysis of promoter activity in Staphylococcus aureus. - 170 p.

Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3110.

Thesis (Ph.D.)--The University of Texas at Dallas, 2003.

Eubacterial pathogens are exposed to a variety of environmental conditions when colonizing and propagating within a host. Their ability to respond quickly to changing conditions is essential for virulence. Delineating the molecular mechanisms that allow these pathogens to utilize diverse genetic systems for growth, protection and the production of virulence factors is key to our ability to defeat these organisms in this age of increased resistance to antibiotics. Of special interest is the human pathogen <italic>Staphylococcus auerus</italic>, the most common cause of nosocomial infections (hospital associated). Collectively, the <italic>S. aureus</italic> strains isolated from around the world have demonstrated resistance to all of the known useful antibiotics. Therefore, it is critical to understand gene regulation for this bacteria and how this regulation is related to pathogenesis.Subjects--Topical Terms:

1017719
Biology, Molecular.

Analysis of promoter activity in Staphylococcus aureus.
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035 $a (UnM)AAI3098547
035 $a AAI3098547
040 $a UnM $c UnM
100 1 $a Ross, Christian Allen. $3 1946064
245 1 0 $a Analysis of promoter activity in Staphylococcus aureus.
300 $a 170 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3110.
500 $a Supervisor: Ronald E. Yasbin.
502 $a Thesis (Ph.D.)--The University of Texas at Dallas, 2003.
520 $a Eubacterial pathogens are exposed to a variety of environmental conditions when colonizing and propagating within a host. Their ability to respond quickly to changing conditions is essential for virulence. Delineating the molecular mechanisms that allow these pathogens to utilize diverse genetic systems for growth, protection and the production of virulence factors is key to our ability to defeat these organisms in this age of increased resistance to antibiotics. Of special interest is the human pathogen <italic>Staphylococcus auerus</italic>, the most common cause of nosocomial infections (hospital associated). Collectively, the <italic>S. aureus</italic> strains isolated from around the world have demonstrated resistance to all of the known useful antibiotics. Therefore, it is critical to understand gene regulation for this bacteria and how this regulation is related to pathogenesis.
520 $a In <italic>S. aureus</italic> the regulation of genes expressed under different culture conditions is understood for only a few regulons, and there are temporal components of this expression that are still not fully understood. Significantly, the transition from exponential to stationary phase marks gross changes in bacterial gene expression and has relevance to pathogenicity as many known secreted virulence factors are expressed at this time.
520 $a To elucidate the pathways involved in the coordinate regulation of stationary phase induced genes, a transposable mutagenesis vector based on Tn<italic> 917</italic> has been developed. This vector carries a transcriptional fusion of two genes which function in <italic>Staphylococcus</italic>. One of these genes is a selectable marker, and the other is a chromogenic indicator. With this vector, it was possible to screen for transposon insertions downstream of promoters. Promoters that were active only under one set of environmental conditions and repressed under another were isolated. Using this system, <italic> cis</italic> and <italic>trans</italic> acting factors responsible for this differential expression, once tagged by the transposon, are amenable to identification by classical methods. In an example described in this thesis, over 5,000 colonies were screened for promoters active primarily in the transition between the exponential and the stationary phases of growth. Once such a promoter was identified, PCR Mutagenesis was used to locate regions of the operator/promoter important for gene expression.
590 $a School code: 0382.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Health Sciences, Pathology. $3 1017854
690 $a 0307
690 $a 0410
690 $a 0369
690 $a 0571
710 2 0 $a The University of Texas at Dallas. $3 1018411
773 0 $t Dissertation Abstracts International $g 64-07B.
790 1 0 $a Yasbin, Ronald E., $e advisor
790 $a 0382
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3098547