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Integrated affinity and separation b...

Jemere, Abebaw Belay.

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Integrated affinity and separation based methods on microfluidic devices.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Integrated affinity and separation based methods on microfluidic devices./
作者:	Jemere, Abebaw Belay.
面頁冊數:	154 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3246.
Contained By:	Dissertation Abstracts International64-07B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ82119
ISBN:	0612821196

Integrated affinity and separation based methods on microfluidic devices.
Jemere, Abebaw Belay.

Integrated affinity and separation based methods on microfluidic devices. - 154 p.

Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3246.

Thesis (Ph.D.)--University of Alberta (Canada), 2003.

The development of miniaturized, microfabricated devices for chemical and biochemical analysis is a new and exciting application of micromachining technology. The ultimate goal of such systems is the development of an automated, portable and high throughput instrument capable of performing an assay on a routine basis. The results presented in chapter 2 of this thesis demonstrate the development and performance of a single channel automated instrument for immunoassay, a technique that is increasingly used in clinical diagnosis and environmental monitoring. The instrument performs the key elements of immuno-chemical analysis: sampling, injection, mixing, separation and detection within 3–5 min. The instrument incorporates a fluidic interface design to facilitate automated sample introduction into an electrokinetic microchip. On-chip mixing, reaction and separation of a protein biological threat agent simulant, ovalbumin, and Cy-5 labeled anti-ovalbumin could be performed with good quantitative results, independent of the operation of the sample introduction interface, which is connected with an external pump.

ISBN: 0612821196Subjects--Topical Terms:

586156
Chemistry, Analytical.

Integrated affinity and separation based methods on microfluidic devices.
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245 1 0 $a Integrated affinity and separation based methods on microfluidic devices.
300 $a 154 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3246.
500 $a Adviser: D. Jed Harrison.
502 $a Thesis (Ph.D.)--University of Alberta (Canada), 2003.
520 $a The development of miniaturized, microfabricated devices for chemical and biochemical analysis is a new and exciting application of micromachining technology. The ultimate goal of such systems is the development of an automated, portable and high throughput instrument capable of performing an assay on a routine basis. The results presented in chapter 2 of this thesis demonstrate the development and performance of a single channel automated instrument for immunoassay, a technique that is increasingly used in clinical diagnosis and environmental monitoring. The instrument performs the key elements of immuno-chemical analysis: sampling, injection, mixing, separation and detection within 3–5 min. The instrument incorporates a fluidic interface design to facilitate automated sample introduction into an electrokinetic microchip. On-chip mixing, reaction and separation of a protein biological threat agent simulant, ovalbumin, and Cy-5 labeled anti-ovalbumin could be performed with good quantitative results, independent of the operation of the sample introduction interface, which is connected with an external pump.
520 $a The thesis also explores the development of a facile method of trapping, rapidly exchanging, and utilizing micro beads within microfluidic devices. A two mask photolithographic and etching process is used to construct micro-weirs in a device substrate. The weirs act to retain the reagent-laden beads while allowing solution to flow through them. Columns ranging from 0.2 mm to 5 mm long were packed with ODS (octadecylsilane) beads and secured with either a “solvent lock” (for solid phase extraction, SPE) or a porous polymer plug technique (for capillary electrochromatography, CEC). Using a 0.2 mm long ODS column for SPE, neutral dyes, amino acids, and peptides were preconcentrated with preconcentration factors as high as 500. For a neutral BODIPY dye, we achieved a detection limit of 70 fM after preconcentration. In CEC, neutrals as well as charged analytes were separated with efficiencies up to 420,000 plates/m. Increasing the column length from 1 to 2 mm yielded &sim;1.8 times more theoretical plates than did the 1 mm column with the same flow rates. van Deemter plots were obtained for the three column lengths, showing increased plate height for the 5 mm length. Indirect fluorescence detection of thiourea and of amino acids is demonstrated using a neutral indicator dye (BODIPY), with a detection limit of 10 μM for amino acids. The packed columns were also extended to show that size exclusion electrochromatography (SEEC) of biopolymers could be performed on chip using electrokinetic pumping. In SEEC, FITC-IgG and FITC-insulin were baseline separated with efficiency up to 139,000 plates/m.
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