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Study of magnetite-immobilized bacte...

Wang, Lei.

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Study of magnetite-immobilized bacterial cell system for removal and recovery of heavy metals from electroplating effluent.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Study of magnetite-immobilized bacterial cell system for removal and recovery of heavy metals from electroplating effluent./
Author:	Wang, Lei.
Description:	315 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-02, Section: B, page: 0898.
Contained By:	Dissertation Abstracts International64-02B.
Subject:	Engineering, Environmental. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3080247

Study of magnetite-immobilized bacterial cell system for removal and recovery of heavy metals from electroplating effluent.
Wang, Lei.

Study of magnetite-immobilized bacterial cell system for removal and recovery of heavy metals from electroplating effluent. - 315 p.

Source: Dissertation Abstracts International, Volume: 64-02, Section: B, page: 0898.

Thesis (Ph.D.)--Hong Kong Polytechnic (People's Republic of China), 2003.

Cu<super>2+</super> and Ni<super>2+</super> are the major heavy metal ions in electroplating wastewater of Hong Kong. The present study was conducted to investigate the removal and recovery of Cu2+ and Ni2+ from electroplating wastewater by bacterial cell biomass.Subjects--Topical Terms:

783782
Engineering, Environmental.

Study of magnetite-immobilized bacterial cell system for removal and recovery of heavy metals from electroplating effluent.
LDR:04312nmm 2200325 4500 001 1857221
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035 $a (UnM)AAI3080247
035 $a AAI3080247
040 $a UnM $c UnM
100 1 $a Wang, Lei. $3 1256221
245 1 0 $a Study of magnetite-immobilized bacterial cell system for removal and recovery of heavy metals from electroplating effluent.
300 $a 315 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-02, Section: B, page: 0898.
500 $a Supervisors: Hong Chua; P. K. Wong.
502 $a Thesis (Ph.D.)--Hong Kong Polytechnic (People's Republic of China), 2003.
520 $a Cu<super>2+</super> and Ni<super>2+</super> are the major heavy metal ions in electroplating wastewater of Hong Kong. The present study was conducted to investigate the removal and recovery of Cu2+ and Ni2+ from electroplating wastewater by bacterial cell biomass.
520 $a A gram-negative bacterium Pseudomonas putida with high Cu<super>2+</super> and Ni<super>2+</super> accumulating capability was isolated from local electroplating effluent. <italic>P. putida</italic> 5-x cells cultured in sulfate limiting medium was found of obviously high Cu<super>2+</super> and Ni<super>2+</super> adsorption capacity compared with those cultured in other media. Higher incubation temperature had a negative effect on Cu<super>2+</super> and Ni<super>2+</super> adsorption of <italic>P. putida</italic> 5-x cell, with effect particularly obvious at the logarithmic growth phase. The bacterial cells harvested in 34&sim;38h and 28–30h had maximum Cu<super>2+</super> and Ni<super> 2+</super> adsorption capacity, respectively.
520 $a The adsorption process of Cu<super>2+</super> and Ni<super>2+</super> by fresh cell consisted of two phases, namely a rapid, metabolism-independent metal ions adsorption phase (biosorption) followed by a slow metabolism-dependent bioaccumulation phase, while pretreated cell only consisted of a rapid, metabolism-independent adsorption phase. About 80% of the total Cu<super>2+</super> and Ni<super> 2+</super> taken up by the fresh bacterial cells were removed within the rapid adsorption phase. The adsorption capacity of <italic>P. putida</italic> 5-x cell was obviously affected by changes of pH. The high the pH, the high the Cu<super>2+</super> and Ni<super>2+</super> adsorption capacities.
520 $a Under suitable conditions, 0.1&sim;0.3 M HCl could effective recover Cu<super>2+</super> and Ni<super>2+</super> from loaded cell biomass with less biomass loss rate. The desorption process was quite rapid, with 95% Cu<super> 2+</super> and 99% Ni<super>2+</super> being desorbed in the first 5 minutes. <italic> P. putida</italic> 5-x cell as biosorbent could be effectively regenerated and reused at least five cycles for removing and recovering Cu<super>2+</super> or Ni<super>2+</super>.
520 $a The adsorption capacity of magnetite immobilized <italic>P. putida</italic> 5-x cell to Cu<super>2+</super> and Ni<super>2+</super> appeared to be much higher than using magnetite alone. The Cu<super>2+</super> and Ni<super>2+ </super> adsorption by both magnetite alone and magnetite immobilized cells obeyed the Freundlich isotherms of QM-Cu = 2.1 Ce-Cu<super> 0.68</super>, QIm-Cu = 11.9 Ce-Cu<super>0.74</super> and QM-Ni = 0.78 Ce-Ni<super>0.79</super>, QM-Ni = 9.7, Ce-Ni<super>0.21</super>.
520 $a The capsules outside the fresh cell of <italic>P. putida</italic> 5-x reduced the adsorption capacity to Cu<super>2+</super> due to the Cu<super> 2+</super>-bridging formed by binding divalent Cu<super>2+</super> on electronegative groups in the capsule, which might induce a conformation change within the capsule, and thus resulted in some metal-binding sites on the cell outer membrane or PEG layer becoming inaccessible for Cu<super>2+</super> binding. (Abstract shortened by UMI.)
590 $a School code: 1170.
650 4 $a Engineering, Environmental. $3 783782
690 $a 0775
710 2 0 $a Hong Kong Polytechnic (People's Republic of China). $3 1249353
773 0 $t Dissertation Abstracts International $g 64-02B.
790 1 0 $a Chua, Hong, $e advisor
790 1 0 $a Wong, P. K., $e advisor
790 $a 1170
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3080247