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Dynamic light scattering microscopy.

Dzakpasu, Rhonda.

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Dynamic light scattering microscopy.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Dynamic light scattering microscopy./
Author:	Dzakpasu, Rhonda.
Description:	103 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-02, Section: B, page: 0793.
Contained By:	Dissertation Abstracts International64-02B.
Subject:	Physics, Optics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3079436

Dynamic light scattering microscopy.
Dzakpasu, Rhonda.

Dynamic light scattering microscopy. - 103 p.

Source: Dissertation Abstracts International, Volume: 64-02, Section: B, page: 0793.

Thesis (Ph.D.)--University of Michigan, 2003.

An optical microscope technique, dynamic light scattering microscopy (DLSM) that images dynamically scattered light fluctuation decay rates is introduced. Using physical optics we show theoretically that within the optical resolution of the microscope, relative motions between scattering centers are sufficient to produce significant phase variations resulting in interference intensity fluctuations in the image plane. The time scale for these intensity fluctuations is predicted. The spatial coherence distance defining the average distance between constructive and destructive interference in the image plane is calculated and compared with the pixel size.Subjects--Topical Terms:

1018756
Physics, Optics.

Dynamic light scattering microscopy.
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035 $a (UnM)AAI3079436
035 $a AAI3079436
040 $a UnM $c UnM
100 1 $a Dzakpasu, Rhonda. $3 1944683
245 1 0 $a Dynamic light scattering microscopy.
300 $a 103 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-02, Section: B, page: 0793.
500 $a Chair: Daniel Axelrod.
502 $a Thesis (Ph.D.)--University of Michigan, 2003.
520 $a An optical microscope technique, dynamic light scattering microscopy (DLSM) that images dynamically scattered light fluctuation decay rates is introduced. Using physical optics we show theoretically that within the optical resolution of the microscope, relative motions between scattering centers are sufficient to produce significant phase variations resulting in interference intensity fluctuations in the image plane. The time scale for these intensity fluctuations is predicted. The spatial coherence distance defining the average distance between constructive and destructive interference in the image plane is calculated and compared with the pixel size.
520 $a We experimentally tested DLSM on polystyrene latex nanospheres and living macrophage cells. In order to record these rapid fluctuations, on a slow progressive scan CCD camera, we used a thin laser line of illumination on the sample such that only a single column of pixels in the CCD camera is illuminated. This allowed the use of the rate of the column-by-column readout transfer process as the acquisition rate of the camera. This manipulation increased the data acquisition rate by at least an order of magnitude in comparison to conventional CCD cameras rates defined by frames/s. Analysis of the observed fluctuations provides information regarding the rates of motion of the scattering centers. These rates, acquired from each position on the sample are used to create a spatial map of the fluctuation decay rates.
520 $a Our experiments show that with this technique, we are able to achieve a good signal-to-noise ratio and can monitor fast intensity fluctuations, on the order of milliseconds. DLSM appears to provide dynamic information about fast motions within cells at a sub-optical resolution scale and provides a new kind of spatial contrast.
590 $a School code: 0127.
650 4 $a Physics, Optics. $3 1018756
650 4 $a Biophysics, General. $3 1019105
690 $a 0752
690 $a 0786
710 2 0 $a University of Michigan. $3 777416
773 0 $t Dissertation Abstracts International $g 64-02B.
790 1 0 $a Axelrod, Daniel, $e advisor
790 $a 0127
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoeng/servlet/advanced?query=3079436