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A multifaceted study of white spot s...

You, Zerong.

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A multifaceted study of white spot syndrome virus (WSSV): A shrimp pathogen.

Record Type:	Electronic resources : Monograph/item
Title/Author:	A multifaceted study of white spot syndrome virus (WSSV): A shrimp pathogen./
Author:	You, Zerong.
Description:	148 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-05, Section: B, page: 2029.
Contained By:	Dissertation Abstracts International64-05B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3090544

A multifaceted study of white spot syndrome virus (WSSV): A shrimp pathogen.
You, Zerong.

A multifaceted study of white spot syndrome virus (WSSV): A shrimp pathogen. - 148 p.

Source: Dissertation Abstracts International, Volume: 64-05, Section: B, page: 2029.

Thesis (Ph.D.)--University of Hawai'i, 2003.

The white spot syndrome virus (WSSV), which has been associated with major viral epizootics, was first identified in China and Taiwan in 1992 and soon spread in shrimp farms all over the world. The mortality rates for shrimp population experiencing WSSV infections can reach 100% within 3 to 10 days from the onset of the symptoms. The WSSV is a double-stranded DNA virus with a genome size of approximately 290 kb.Subjects--Topical Terms:

1017734
Biology, Microbiology.

A multifaceted study of white spot syndrome virus (WSSV): A shrimp pathogen.
LDR:03143nmm 2200289 4500 001 1855764
005 20040629073631.5
008 130614s2003 eng d
035 $a (UnM)AAI3090544
035 $a AAI3090544
040 $a UnM $c UnM
100 1 $a You, Zerong. $3 1943565
245 1 0 $a A multifaceted study of white spot syndrome virus (WSSV): A shrimp pathogen.
300 $a 148 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-05, Section: B, page: 2029.
500 $a Chair: Philip C. Loh.
502 $a Thesis (Ph.D.)--University of Hawai'i, 2003.
520 $a The white spot syndrome virus (WSSV), which has been associated with major viral epizootics, was first identified in China and Taiwan in 1992 and soon spread in shrimp farms all over the world. The mortality rates for shrimp population experiencing WSSV infections can reach 100% within 3 to 10 days from the onset of the symptoms. The WSSV is a double-stranded DNA virus with a genome size of approximately 290 kb.
520 $a In this study, a WSSV strain was isolated from <italic>P. chinesis</italic> from Qindao, China. The morphology of this WSSV isolate was examined by electron microscope (EM) and compared with other WSSV isolates.
520 $a A cDNA library was constructed from white spot syndrome virus (WSSV) infected penaeid shrimp. cDNA clones with WSSV sequence insert were isolated, and twelve clones were sequenced and analyzed. By comparing with the DNA sequence in the GenBank, cDNA clones containing sequence identical to that of WSSV envelope protein VP28 and nucleoprotein VP 15 were identified. Poly(A) sites on the mRNAs of VP28 and VP15 were identified. Genes encoding major viral structural proteins VP28, VP26, VP24, VP19 and VP15 from five WSSV isolates were sequenced and compared with that of other sequences of WSSV strains available in the GenBank. The genes encoding these major viral structural proteins are identical among WSSV strains isolated from different shrimp species and/or geographical areas. Gene probes developed based on the DNA sequences of viral structural proteins can be used for WSSV diagnosis/detection.
520 $a A truncated version of the white spot syndrome virus (WSSV) 27.5kDa envelope protein was expressed as a histidine tag fusion protein in <italic>Escherichia coli</italic>. The bacterial expression system allowed the production of up to 10 mg of purified recombinant protein per liter of bacterial culture. Antiserum from a rabbit immunized with the recombinant protein was found to recognize the 27.5kDa viral envelope protein of WSSV isolated from different geographic regions. The antiserum did not recognize any of the other known WSSV structural proteins. A sensitive immuno-dot assay for WSSV was developed using the specific rabbit polyclonal antiserum. A wet-format dipstick assay model was developed for rapid WSSV detection.
590 $a School code: 0085.
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0410
710 2 0 $a University of Hawai'i. $3 1900371
773 0 $t Dissertation Abstracts International $g 64-05B.
790 1 0 $a Loh, Philip C., $e advisor
790 $a 0085
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3090544