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Adjuvanticity of chicken interleukin...

Hu, Wanping.

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Adjuvanticity of chicken interleukin-2 in DNA vaccines and protein vaccines delivered via microspheres.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Adjuvanticity of chicken interleukin-2 in DNA vaccines and protein vaccines delivered via microspheres./
Author:	Hu, Wanping.
Description:	103 p.
Notes:	Source: Dissertation Abstracts International, Volume: 62-12, Section: B, page: 5631.
Contained By:	Dissertation Abstracts International62-12B.
Subject:	Health Sciences, Immunology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3037085
ISBN:	0493504494

Adjuvanticity of chicken interleukin-2 in DNA vaccines and protein vaccines delivered via microspheres.
Hu, Wanping.

Adjuvanticity of chicken interleukin-2 in DNA vaccines and protein vaccines delivered via microspheres. - 103 p.

Source: Dissertation Abstracts International, Volume: 62-12, Section: B, page: 5631.

Thesis (Ph.D.)--Wayne State University, 2001.

To determine the immune modulating effects of chicken interleukin-2 (chIL-2), a cytokine 24% identical to mammalian IL-2 at the amino acid level, we undertook vaccination studies in chickens utilizing a truncated Marek's Disease virus (MDV) glycoprotein B (gp49, gB) with chIL-2 as adjuvant, administered as plasmid DNA or recombinant protein. To maintain close proximity between antigen and cytokine, the respective plasmids were co-administered by attenuated <italic> ΔactA Listeria monocytogenes</italic> (<italic>ΔactA</italic> LM), gene gun inoculation or needle injection, and recombinant gB and chIL-2 protein were co-delivered by three types of microspheres: 5 micron polystyrene latex microspheres (PLM), biodegradable polylactic acid microspheres (PLAM) and 1 micron aldehyde/sulfate polystyrene latex microspheres (A/SPLM). Chickens were immunized at 3 weeks with plasmid DNA or protein, and their immune responses to the antigen and recall responses to live virus were evaluated by ELISA and T cell proliferative assays. Results showed that <italic>ΔactA</italic> LM could serve as DNA vaccine delivery vehicle in chickens. The specific humoral responses were significantly enhanced by co-administration of chIL-2 by gene gun, intramuscular plasmid injection and all three types of microspheres. The one micron A/SPLM were the most effective vehicles in inducing T cell proliferative responses, and co-delivery of chIL-2 significantly enhanced these responses. In summary, chIL-2 was proven, for the first time, to be an effective adjuvant for antibody and T cell proliferative responses. Another important finding was that latex microspheres were an excellent vehicle for co-delivering microgram quantities of recombinant proteins with chIL-2. These microspheres should be useful for testing the adjuvanticity of other cytokines and costimulatory molecules.

ISBN: 0493504494Subjects--Topical Terms:

1017716
Health Sciences, Immunology.

Adjuvanticity of chicken interleukin-2 in DNA vaccines and protein vaccines delivered via microspheres.
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245 1 0 $a Adjuvanticity of chicken interleukin-2 in DNA vaccines and protein vaccines delivered via microspheres.
300 $a 103 p.
500 $a Source: Dissertation Abstracts International, Volume: 62-12, Section: B, page: 5631.
500 $a Adviser: Roy S. Sundick.
502 $a Thesis (Ph.D.)--Wayne State University, 2001.
520 $a To determine the immune modulating effects of chicken interleukin-2 (chIL-2), a cytokine 24% identical to mammalian IL-2 at the amino acid level, we undertook vaccination studies in chickens utilizing a truncated Marek's Disease virus (MDV) glycoprotein B (gp49, gB) with chIL-2 as adjuvant, administered as plasmid DNA or recombinant protein. To maintain close proximity between antigen and cytokine, the respective plasmids were co-administered by attenuated <italic> ΔactA Listeria monocytogenes</italic> (<italic>ΔactA</italic> LM), gene gun inoculation or needle injection, and recombinant gB and chIL-2 protein were co-delivered by three types of microspheres: 5 micron polystyrene latex microspheres (PLM), biodegradable polylactic acid microspheres (PLAM) and 1 micron aldehyde/sulfate polystyrene latex microspheres (A/SPLM). Chickens were immunized at 3 weeks with plasmid DNA or protein, and their immune responses to the antigen and recall responses to live virus were evaluated by ELISA and T cell proliferative assays. Results showed that <italic>ΔactA</italic> LM could serve as DNA vaccine delivery vehicle in chickens. The specific humoral responses were significantly enhanced by co-administration of chIL-2 by gene gun, intramuscular plasmid injection and all three types of microspheres. The one micron A/SPLM were the most effective vehicles in inducing T cell proliferative responses, and co-delivery of chIL-2 significantly enhanced these responses. In summary, chIL-2 was proven, for the first time, to be an effective adjuvant for antibody and T cell proliferative responses. Another important finding was that latex microspheres were an excellent vehicle for co-delivering microgram quantities of recombinant proteins with chIL-2. These microspheres should be useful for testing the adjuvanticity of other cytokines and costimulatory molecules.
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