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Characterization of white spot syndr...

Wang, Qiong.

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Characterization of white spot syndrome virus of penaeid shrimp: Genomic cloning and sequencing, structural protein analyzing and sequencing, genetic diversity, pathology and virulence.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Characterization of white spot syndrome virus of penaeid shrimp: Genomic cloning and sequencing, structural protein analyzing and sequencing, genetic diversity, pathology and virulence./
作者:	Wang, Qiong.
面頁冊數:	203 p.
附註:	Source: Dissertation Abstracts International, Volume: 60-04, Section: B, page: 1361.
Contained By:	Dissertation Abstracts International60-04B.
標題:	Agriculture, Animal Pathology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9927506
ISBN:	0599272139

Characterization of white spot syndrome virus of penaeid shrimp: Genomic cloning and sequencing, structural protein analyzing and sequencing, genetic diversity, pathology and virulence.
Wang, Qiong.

Characterization of white spot syndrome virus of penaeid shrimp: Genomic cloning and sequencing, structural protein analyzing and sequencing, genetic diversity, pathology and virulence. - 203 p.

Source: Dissertation Abstracts International, Volume: 60-04, Section: B, page: 1361.

Thesis (Ph.D.)--The University of Arizona, 1999.

The purpose of this dissertation was to characterize virulence, genomic and protein composition of a newly emerged virus of penaeid shrimp: white spot syndrome virus (WSSV).

ISBN: 0599272139Subjects--Topical Terms:

1021764
Agriculture, Animal Pathology.

Characterization of white spot syndrome virus of penaeid shrimp: Genomic cloning and sequencing, structural protein analyzing and sequencing, genetic diversity, pathology and virulence.
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245 1 0 $a Characterization of white spot syndrome virus of penaeid shrimp: Genomic cloning and sequencing, structural protein analyzing and sequencing, genetic diversity, pathology and virulence.
300 $a 203 p.
500 $a Source: Dissertation Abstracts International, Volume: 60-04, Section: B, page: 1361.
500 $a Director: Donald V. Lightner.
502 $a Thesis (Ph.D.)--The University of Arizona, 1999.
520 $a The purpose of this dissertation was to characterize virulence, genomic and protein composition of a newly emerged virus of penaeid shrimp: white spot syndrome virus (WSSV).
520 $a A partial genomic library, covering approximately 30–50% of the genome, of WSSV isolated from crayfish <italic>Orconectes punctimanus</italic>, was constructed by digesting viral DNA with endonuclease ClaI and cloning into the system pBluescript-JM109. Three viral inserts of approximately 2.2 kb, 2.8 kb and 6.3 kb, named as QW245, CR44, and QW237 respectively, were sequenced and analyzed.
520 $a Six geographic isolates of WSSV, from China, India, Thailand, South Carolina, Texas, as well as from crayfish obtained from the US National Zoo in Washington D.C. were compared by electron microscopy (TEM) and SDS-PAGE. All viral isolates contained three major polypeptides of 25, 23 and 19 kDa. A fourth major polypeptide at the 14.5 kDa position was observed in four of the viral isolates. The 19 kDa polypeptide of the crayfish WSSV appeared larger in size than that of the other isolates. Amino acid composition of four of the major structural polypeptides of the South Carolina WSSV was analyzed. The NH<sub>2</sub> terminal amino acids of the 25, 23 and 14.5 kDa polypeptides of the SC WSSV were sequenced as MDLSFTLSVVTA, MEFGNLTNLDVA, and VARGGKTKGRRG, respectively.
520 $a The genomic composition of the six geographic isolates of WSSV were compared by combining the methods of restriction analysis using nine endonucleases AccI, BglII, ClaI, BamHI, EcoRI, HindII, HaeI, SacI, XhoI and Southern blot hybridization applying three digoxigenin-11-dUTP labeled WSSV genomic probes LN4, C42 and A6. No distinctive difference among five WSSV isolated from penaeid shrimp was detected; differences were observed in the crayfish isolate of WSSV.
520 $a The virulence of the six geographic isolates of WSSV were compared by <italic> per os</italic> challenge of <italic>Litopenaeus vannamei</italic> postlarvae and juveniles, and <italic>Farfantepenaeus duorarum</italic> juveniles. The Texas WSSV caused higher and more rapid mortalities; the crayfish WSSV caused lower and less rapid mortalities. <italic>L. vannamei</italic> postlarvae and juveniles were very susceptible to WSSV infection, while <italic>Fa. duorarum </italic> juveniles showed moderate resistance.
590 $a School code: 0009.
650 4 $a Agriculture, Animal Pathology. $3 1021764
650 4 $a Agriculture, Fisheries and Aquaculture. $3 1020913
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0476
690 $a 0792
690 $a 0410
710 2 0 $a The University of Arizona. $3 1017508
773 0 $t Dissertation Abstracts International $g 60-04B.
790 1 0 $a Lightner, Donald V., $e advisor
790 $a 0009
791 $a Ph.D.
792 $a 1999
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9927506