東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Molecular recognition of proteins an...

Ng, Ho Leung.

Linked to FindBook

Google Book

Amazon

博客來

Molecular recognition of proteins and DNA.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Molecular recognition of proteins and DNA./
Author:	Ng, Ho Leung.
Description:	73 p.
Notes:	Source: Dissertation Abstracts International, Volume: 63-01, Section: B, page: 0129.
Contained By:	Dissertation Abstracts International63-01B.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3040170
ISBN:	0493535411

Molecular recognition of proteins and DNA.
Ng, Ho Leung.

Molecular recognition of proteins and DNA. - 73 p.

Source: Dissertation Abstracts International, Volume: 63-01, Section: B, page: 0129.

Thesis (Ph.D.)--University of California, Los Angeles, 2001.

A drug has been designed to bind specific subsets of DNA sequences. The monoimidazole lexitropsin was intended to distinguish between AT and GC base pairs. However, the crystal structure of the DNA-drug complex reveals that the lexitropsin is an indiscriminate binder. Its two positively charged tails draw it into the negative electrostatic potential of deep minor grooves associated with AT-rich sequences.

ISBN: 0493535411Subjects--Topical Terms:

1019105
Biophysics, General.

Molecular recognition of proteins and DNA.
LDR:02945nmm 2200325 4500 001 1851670
005 20051215074957.5
008 130614s2001 eng d
020 $a 0493535411
035 $a (UnM)AAI3040170
035 $a AAI3040170
040 $a UnM $c UnM
100 1 $a Ng, Ho Leung. $3 1939553
245 1 0 $a Molecular recognition of proteins and DNA.
300 $a 73 p.
500 $a Source: Dissertation Abstracts International, Volume: 63-01, Section: B, page: 0129.
500 $a Co-Chairs: David Eisenberg; Richard Dickerson.
502 $a Thesis (Ph.D.)--University of California, Los Angeles, 2001.
520 $a A drug has been designed to bind specific subsets of DNA sequences. The monoimidazole lexitropsin was intended to distinguish between AT and GC base pairs. However, the crystal structure of the DNA-drug complex reveals that the lexitropsin is an indiscriminate binder. Its two positively charged tails draw it into the negative electrostatic potential of deep minor grooves associated with AT-rich sequences.
520 $a The relationship between DNA sequence and structure is also demonstrated in the first crystal structure of an A/B-DNA intermediate. Guanine rich sequences have long been known to facilitate the transition of B DNA to A DNA. The sequence CATGGGCCCATG, with three consecutive guanines, crystallizes as an A/B-DNA intermediate. In contrast, the similar sequence CATGGCCATG, with two consecutive guanines, crystallizes as B DNA. This unusual structure is examined by quantum mechanics ab initio calculations, solvation simulation, and molecular dynamics simulations. The properties of this intermediate favor the Calladine-Drew "Slide first, roll later" pathway for the B → A DNA transition. Energetic calculations suggest that DNA is a much more dynamic molecule than many have assumed.
520 $a In the post-genomic era, new bioinformatics tools are needed to take advantage of our newfound wealth of data. We have developed an algorithm that predicts protein functions and protein-protein interactions. The Rosetta Stone method relies on the correlation between protein function and genetic fusion events. Two proteins are more likely to be related in function if they can be found as a fused gene in an organism.
520 $a In many cases, proteins related in function share common structural or sequence elements. Actin binding proteins were searched for such motifs. Two motifs common to the villin headpiece, calponin homology, and gelsolin S1 domains were found. These motifs correlate with residues that had previously been determined to be critical for actin binding.
590 $a School code: 0031.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Biology, Molecular. $3 1017719
690 $a 0786
690 $a 0307
710 2 0 $a University of California, Los Angeles. $3 626622
773 0 $t Dissertation Abstracts International $g 63-01B.
790 1 0 $a Eisenberg, David, $e advisor
790 1 0 $a Dickerson, Richard, $e advisor
790 $a 0031
791 $a Ph.D.
792 $a 2001
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3040170