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TRANSCRIPTION REGULATION AND GENETIC...

ADAMS, CRAIG W.

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TRANSCRIPTION REGULATION AND GENETIC STRUCTURE OF THE ILVGEDA OPERON IN ESCHERICHIA COLI K12.

Record Type:	Electronic resources : Monograph/item
Title/Author:	TRANSCRIPTION REGULATION AND GENETIC STRUCTURE OF THE ILVGEDA OPERON IN ESCHERICHIA COLI K12./
Author:	ADAMS, CRAIG W.
Description:	196 p.
Notes:	Source: Dissertation Abstracts International, Volume: 44-02, Section: B, page: 0405.
Contained By:	Dissertation Abstracts International44-02B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8313784

TRANSCRIPTION REGULATION AND GENETIC STRUCTURE OF THE ILVGEDA OPERON IN ESCHERICHIA COLI K12.
ADAMS, CRAIG W.

TRANSCRIPTION REGULATION AND GENETIC STRUCTURE OF THE ILVGEDA OPERON IN ESCHERICHIA COLI K12. - 196 p.

Source: Dissertation Abstracts International, Volume: 44-02, Section: B, page: 0405.

Thesis (Ph.D.)--University of California, Irvine, 1983.

The ilvGEDA genetic cluster of Escherichia coli codes for four enzymes necessary for the biosynthesis of three structurally similar amino acids: isoleucine, leucine, and valine. Historically there has been a substantial amount of confusion and controversy regarding the genetic structure and transcriptional regulation of these enzymes. Much of the controversy and confusion regarding the genetic and regulatory structure of this gene cluster has revolved around the ilvO, the ilvJ and the ilxA genetic loci.Subjects--Topical Terms:

1017730
Biology, Genetics.

TRANSCRIPTION REGULATION AND GENETIC STRUCTURE OF THE ILVGEDA OPERON IN ESCHERICHIA COLI K12.
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035 $a (UnM)AAI8313784
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040 $a UnM $c UnM
100 1 $a ADAMS, CRAIG W. $3 1939064
245 1 0 $a TRANSCRIPTION REGULATION AND GENETIC STRUCTURE OF THE ILVGEDA OPERON IN ESCHERICHIA COLI K12.
300 $a 196 p.
500 $a Source: Dissertation Abstracts International, Volume: 44-02, Section: B, page: 0405.
502 $a Thesis (Ph.D.)--University of California, Irvine, 1983.
520 $a The ilvGEDA genetic cluster of Escherichia coli codes for four enzymes necessary for the biosynthesis of three structurally similar amino acids: isoleucine, leucine, and valine. Historically there has been a substantial amount of confusion and controversy regarding the genetic structure and transcriptional regulation of these enzymes. Much of the controversy and confusion regarding the genetic and regulatory structure of this gene cluster has revolved around the ilvO, the ilvJ and the ilxA genetic loci.
520 $a Immunologic and kinetic analysis of the ilvE gene product, transaminase B, demonstrates that this enzyme is the only ilv encoded transaminase. These analyses suggest that an enzymatic activity previously assigned to the gene, ilvJ, is in fact part of the activity of the ilvE gene product which is capable of utilizing multiple substrates.
520 $a The genetically defined ilvO locus has been thought to be a regulatory site of the ilv operon. DNA sequence analysis shows that this genetic locus is not a regulatory site but a naturally occurring 2 base pair deletion which indirectly affects ilv regulation.
520 $a A variety of biochemical and genetic evidence has been collected which suggests that the ilvA gene product is directly involved in ilv regulation. Examination of the in vivo effects of the threonine deaminase expression, produced in trans from a lac-ilvA transcription fusion plasmid, indicates that threonine deaminase is not a trans acting regulatory element for the ilvGEDA operon.
520 $a RNA fingerprint analysis of the in vivo transcription products of the ilvGEDA attenuator region demonstrates that this operon is regulated by an attenuation mechanism. Additionally, this analysis shows that only one of the two ilv promoters seen in vitro initiates significant transcription in vivo. However, deletion analysis of this tandem promoter region suggests that there is a requirement for the upstream promoter. This analysis implies that the requirement for the upstream promoter is due to a cooperative enhancement of transcription mediated by protein-protein interaction between two RNA polymerases at the tandem ilv promoter site (ilv P1 and ilv P2). Thus, RNA polymerase functions as a positive activator by binding to a region (ilv P1) located 72 base pairs upstream of the activated promoter (ilv P2).
590 $a School code: 0030.
650 4 $a Biology, Genetics. $3 1017730
690 $a 0369
710 2 0 $a University of California, Irvine. $3 705821
773 0 $t Dissertation Abstracts International $g 44-02B.
790 $a 0030
791 $a Ph.D.
792 $a 1983
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8313784