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An evaluation of the microbiological...

Mohammed, Renuka Lisa.

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An evaluation of the microbiological quality of sandy beaches: A novel approach.

Record Type:	Electronic resources : Monograph/item
Title/Author:	An evaluation of the microbiological quality of sandy beaches: A novel approach./
Author:	Mohammed, Renuka Lisa.
Description:	135 p.
Notes:	Source: Masters Abstracts International, Volume: 42-05, page: 1627.
Contained By:	Masters Abstracts International42-05.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1418593
ISBN:	0496233661

An evaluation of the microbiological quality of sandy beaches: A novel approach.
Mohammed, Renuka Lisa.

An evaluation of the microbiological quality of sandy beaches: A novel approach. - 135 p.

Source: Masters Abstracts International, Volume: 42-05, page: 1627.

Thesis (M.S.)--Florida Atlantic University, 2004.

A novel, 'near-real' time technique; peptide nucleic acid chemilumiscent in situ hybridization (PNA CISH), was developed and validated for detecting, enumerating and identifying potential indicators (Staphyloccus aureus and Pseudomonas aeruginosa) of beach quality. To understand the dynamics of bacterial indicators a plethora of physical, chemical and biological parameters were tested under field and lab conditions. Escherichia coli tagged with green fluorescence protein (GFP) was used to assess the impact of wave energy and rainfall on seawater counts.

ISBN: 0496233661Subjects--Topical Terms:

1017734
Biology, Microbiology.

An evaluation of the microbiological quality of sandy beaches: A novel approach.
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008 130614s2004 eng d
020 $a 0496233661
035 $a (UnM)AAI1418593
035 $a AAI1418593
040 $a UnM $c UnM
100 1 $a Mohammed, Renuka Lisa. $3 1937011
245 1 3 $a An evaluation of the microbiological quality of sandy beaches: A novel approach.
300 $a 135 p.
500 $a Source: Masters Abstracts International, Volume: 42-05, page: 1627.
500 $a Adviser: Nwadiuto Esiobu.
502 $a Thesis (M.S.)--Florida Atlantic University, 2004.
520 $a A novel, 'near-real' time technique; peptide nucleic acid chemilumiscent in situ hybridization (PNA CISH), was developed and validated for detecting, enumerating and identifying potential indicators (Staphyloccus aureus and Pseudomonas aeruginosa) of beach quality. To understand the dynamics of bacterial indicators a plethora of physical, chemical and biological parameters were tested under field and lab conditions. Escherichia coli tagged with green fluorescence protein (GFP) was used to assess the impact of wave energy and rainfall on seawater counts.
520 $a PNA CISH and plate counts correlated strongly (r = 0.94 to r = 0.86). Temperature, salinity, nutrient availability and predation significantly influenced the survival of indicators. Rainfall and wave energy affected the re-suspension of bacteria from sand onto overlying water. Overall PNA CISH provides a reliable rapid method for beach monitoring. The implications of beach topography and sampling time on the numbers of Clostridium perfringens and other potential indicators are discussed. Data suggest a revision of policy for tropical sandy beaches.
590 $a School code: 0119.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Environmental Sciences. $3 676987
650 4 $a Health Sciences, Public Health. $3 1017659
690 $a 0410
690 $a 0768
690 $a 0573
710 2 0 $a Florida Atlantic University. $3 1017837
773 0 $t Masters Abstracts International $g 42-05.
790 1 0 $a Esiobu, Nwadiuto, $e advisor
790 $a 0119
791 $a M.S.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1418593