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Investigating the reduction of estro...

Wozei, Eleanor.

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Investigating the reduction of estrogenic activity by activated sludge.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Investigating the reduction of estrogenic activity by activated sludge./
Author:	Wozei, Eleanor.
Description:	155 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4763.
Contained By:	Dissertation Abstracts International65-09B.
Subject:	Engineering, Environmental. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3147052
ISBN:	0496055801

Investigating the reduction of estrogenic activity by activated sludge.
Wozei, Eleanor.

Investigating the reduction of estrogenic activity by activated sludge. - 155 p.

Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4763.

Thesis (Ph.D.)--University of California, Berkeley, 2004.

17beta-estradiol (E2) and its metabolites are found in municipal wastewater effluent and receiving waters in many parts of the world. They are present in trace concentrations and will require innovative engineering solutions to monitor and remove in wastewater treatment processes designed to remove higher concentrations of contaminants.

ISBN: 0496055801Subjects--Topical Terms:

783782
Engineering, Environmental.

Investigating the reduction of estrogenic activity by activated sludge.
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035 $a (UnM)AAI3147052
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100 1 $a Wozei, Eleanor. $3 1932905
245 1 0 $a Investigating the reduction of estrogenic activity by activated sludge.
300 $a 155 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4763.
500 $a Chair: Slawomir W. Hermanowicz.
502 $a Thesis (Ph.D.)--University of California, Berkeley, 2004.
520 $a 17beta-estradiol (E2) and its metabolites are found in municipal wastewater effluent and receiving waters in many parts of the world. They are present in trace concentrations and will require innovative engineering solutions to monitor and remove in wastewater treatment processes designed to remove higher concentrations of contaminants.
520 $a Activated sludge systems are used to treat wastewater in many parts of the world. With total suspended solids (TSS) concentrations up to 10 g/L and a versatile mixed culture of microorganisms, they present an ideal system for reduction of estrogens by sorption and microbial metabolism.
520 $a A yeast construct transfected with human estrogen receptor (ERalpha) was used to adapt a sensitive yeast assay and develop a protocol to measure estrogenic activity in wastewater. The assay combined detection of estrognic activity to as low as 0.03 ngE2/L and greatest quantitative sensitivity between 250 and 2900 ngE2/L.
520 $a Batch experiments were carried out with sludge solids concentrations ranging from 1 to 5 gTSS/L and E2 spiked doses of 100 ng/L, 100 mug/L and 20 mg/L. The assay was used to measure reduction of total estrogenic activity from the sludge supernatant over a 15-day period after which the sludge was re-spiked with E2 and further monitored to check for reduction by pre-exposed sludge. The reduction was generally increased by increasing sludge solids. For 100 ngE2/L there was >40% reduction of estrogenic activity within 15 days which improved to >70% by pre-exposing the sludge. The estrogenic activity produced by a dose of 100 mug/L was readily removed within 15 days. However, re-spiking the sludge with the same concentration caused some sludges to lose reduction capacity. The same observation was made at 20 mg/L dose where although up to 70% reductions could be obtained with >2 gTSS/L, only some sludges showed improved reduction after re-spiking.
520 $a Keeping the sludge aerated and viable by mixing was found to enhance reduction. This was confirmed using static control experiments and sterile sludge, and by comparing short-term solid versus liquid phase partitioning. Unmixed sludge removed estrogenic activity slower and only solids concentrations <1 gTSS/L maintained estrogenic activity reduction capacity, probably due to low oxygen demand.
590 $a School code: 0028.
650 4 $a Engineering, Environmental. $3 783782
650 4 $a Engineering, Sanitary and Municipal. $3 1018731
650 4 $a Engineering, Civil. $3 783781
690 $a 0775
690 $a 0554
690 $a 0543
710 2 0 $a University of California, Berkeley. $3 687832
773 0 $t Dissertation Abstracts International $g 65-09B.
790 1 0 $a Hermanowicz, Slawomir W., $e advisor
790 $a 0028
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3147052