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Biological and molecular characteriz...

Kusumah, Yayi Munara.

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Biological and molecular characterization of Ascovirus isolates from Indonesia and the United States.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Biological and molecular characterization of Ascovirus isolates from Indonesia and the United States./
Author:	Kusumah, Yayi Munara.
Description:	99 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-12, Section: B, page: 6157.
Contained By:	Dissertation Abstracts International65-12B.
Subject:	Biology, Entomology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3159403
ISBN:	0496926233

Biological and molecular characterization of Ascovirus isolates from Indonesia and the United States.
Kusumah, Yayi Munara.

Biological and molecular characterization of Ascovirus isolates from Indonesia and the United States. - 99 p.

Source: Dissertation Abstracts International, Volume: 65-12, Section: B, page: 6157.

Thesis (Ph.D.)--Clemson University, 2004.

To study the ecology of ascoviruses in the field, collections of noctuid larvae were conducted in cotton, soybean and corn during the summer seasons of 2000, 2001 and 2002. Collections in 2000 were conducted at the Edisto REC and Sandifer Field cotton plantations, in 2001 at the Edisto REC and Bamberg county, and in 2002 at the Edisto REC and the Coastal REC. Due to a prolonged drought which lasted from 1998 to 2001, populations of noctuid pests in both Edisto REC and Sandifer Field were very low. Populations of parasitoids were not present and during this season, ascovirus infection was not recorded.

ISBN: 0496926233Subjects--Topical Terms:

1018619
Biology, Entomology.

Biological and molecular characterization of Ascovirus isolates from Indonesia and the United States.
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020 $a 0496926233
035 $a (UnM)AAI3159403
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100 1 $a Kusumah, Yayi Munara. $3 1931793
245 1 0 $a Biological and molecular characterization of Ascovirus isolates from Indonesia and the United States.
300 $a 99 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-12, Section: B, page: 6157.
500 $a Adviser: Gerald R. Carner.
502 $a Thesis (Ph.D.)--Clemson University, 2004.
520 $a To study the ecology of ascoviruses in the field, collections of noctuid larvae were conducted in cotton, soybean and corn during the summer seasons of 2000, 2001 and 2002. Collections in 2000 were conducted at the Edisto REC and Sandifer Field cotton plantations, in 2001 at the Edisto REC and Bamberg county, and in 2002 at the Edisto REC and the Coastal REC. Due to a prolonged drought which lasted from 1998 to 2001, populations of noctuid pests in both Edisto REC and Sandifer Field were very low. Populations of parasitoids were not present and during this season, ascovirus infection was not recorded.
520 $a The data collected during 2001 and 2002 seasons show that C. marginiventris and M. croceipes populations were relatively high when ascovirus infections were observed in S. exigua and H. zea. Ascovirus infections in 2001 and 2002 were recorded from S. exigua, H. zea and H. virescens . This suggests the possible role of parasitoids in transmission of ascovirus in the field.
520 $a Seven ascovirus isolates were used in biological, morphological and molecular characterization studies. They were isolated from larva of S. exigua collected in Indonesia (INDO1 and INDO2), larvae of H. virescens (SC1), H. zea (SC2), H. virescens (SC3), and S. frugiperda (SC4) collected in South Carolina, and larvae of S. frugiperda collected in Georgia in 1982 (Sf 82-126). Laboratory-reared S. exigua, T. ni, S. frugiperda, H. zea, L. dispar, A. gemmatalis and P. includens were used in these studies. Third instar larvae of each species were inoculated with each ascovirus isolate.
520 $a Isolate SC4 was the most restricted in host range, producing fully developed infections only in S. frugiperda. Isolates SC1, SC2, and SC3 had similar host ranges except that SC2 was more virulent to S. frugiperda than to SC1 and SC3. These three isolates caused infection in S. exigua, T. ni, S. frugiperda, H. zea, and P. includens. SC1 and SC2 isolates produced few infections to A. gemmatalis and did not infect L. dispar. Isolates INDO1 and INDO2 were highly virulent against S. exigua, H. zea and P. includens, showed very low infection in S. frugiperda, and no infection in T. ni, L. dispar and A. gemmatalis. Based on host range studies, INDO1, INDO2, SC1, SC2, and SC3 are closely related to isolates from H. zea and H. virescens as reported by Hamm et al. (1998), and SC 4 is closely related to the isolate from S. frugiperda of Hamm et al. (1998). (Abstract shortened by UMI.)
590 $a School code: 0050.
650 4 $a Biology, Entomology. $3 1018619
650 4 $a Biology, Molecular. $3 1017719
690 $a 0353
690 $a 0307
710 2 0 $a Clemson University. $3 997173
773 0 $t Dissertation Abstracts International $g 65-12B.
790 1 0 $a Carner, Gerald R., $e advisor
790 $a 0050
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3159403