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Expression in plants and immunogenic...

Bailey, Andrea Elizabeth.

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Expression in plants and immunogenicity of a fusion protein containing an epitope from the swine transmissible gastroenteritis virus S protein.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Expression in plants and immunogenicity of a fusion protein containing an epitope from the swine transmissible gastroenteritis virus S protein./
Author:	Bailey, Andrea Elizabeth.
Description:	140 p.
Notes:	Source: Masters Abstracts International, Volume: 39-04, page: 1087.
Contained By:	Masters Abstracts International39-04.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ56301
ISBN:	0612563014

Expression in plants and immunogenicity of a fusion protein containing an epitope from the swine transmissible gastroenteritis virus S protein.
Bailey, Andrea Elizabeth.

Expression in plants and immunogenicity of a fusion protein containing an epitope from the swine transmissible gastroenteritis virus S protein. - 140 p.

Source: Masters Abstracts International, Volume: 39-04, page: 1087.

Thesis (M.Sc.)--University of Guelph (Canada), 2001.

One interesting possibility offered by recent advances in plant biotechnology is the production of antigens in plants for use as orally administered vaccines. In this work, a subunit vaccine was designed for production in transgenic alfalfa. A genetic construct encoding a fusion protein containing a neutralizing epitope from the transmissible gastroenteritis virus (TGEV) was generated and introduced into tobacco and alfalfa. The fusion partner was a portion of the pollen-specific alfalfa protein PO2. This PO2-D protein was detected in plants using antibodies to PO2, and was found to be more extensively glycosylated in alfalfa leaves than in tobacco leaves. However, as both the alfalfa and tobacco plants matured, the expression of PO2-D diminished. To determine the immunogenicity of PO2-D, crude protein extracts from transgenic leaf tissue were injected intraperitoneally (i.p.) into mice and the serum antibodies examined for reactivity with TGEV. Both TGEV-specific and TGEV neutralizing antibodies were detected.

ISBN: 0612563014Subjects--Topical Terms:

1017719
Biology, Molecular.

Expression in plants and immunogenicity of a fusion protein containing an epitope from the swine transmissible gastroenteritis virus S protein.
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100 1 $a Bailey, Andrea Elizabeth. $3 1929187
245 1 0 $a Expression in plants and immunogenicity of a fusion protein containing an epitope from the swine transmissible gastroenteritis virus S protein.
300 $a 140 p.
500 $a Source: Masters Abstracts International, Volume: 39-04, page: 1087.
500 $a Adviser: L. R. Erickson.
502 $a Thesis (M.Sc.)--University of Guelph (Canada), 2001.
520 $a One interesting possibility offered by recent advances in plant biotechnology is the production of antigens in plants for use as orally administered vaccines. In this work, a subunit vaccine was designed for production in transgenic alfalfa. A genetic construct encoding a fusion protein containing a neutralizing epitope from the transmissible gastroenteritis virus (TGEV) was generated and introduced into tobacco and alfalfa. The fusion partner was a portion of the pollen-specific alfalfa protein PO2. This PO2-D protein was detected in plants using antibodies to PO2, and was found to be more extensively glycosylated in alfalfa leaves than in tobacco leaves. However, as both the alfalfa and tobacco plants matured, the expression of PO2-D diminished. To determine the immunogenicity of PO2-D, crude protein extracts from transgenic leaf tissue were injected intraperitoneally (i.p.) into mice and the serum antibodies examined for reactivity with TGEV. Both TGEV-specific and TGEV neutralizing antibodies were detected.
590 $a School code: 0081.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Health Sciences, Immunology. $3 1017716
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710 2 0 $a University of Guelph (Canada). $3 1018650
773 0 $t Masters Abstracts International $g 39-04.
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791 $a M.Sc.
792 $a 2001
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