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Cloning and evaluation of gene promo...

Gaitan, Alvaro Leon.

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Cloning and evaluation of gene promoters in Coffea spp.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Cloning and evaluation of gene promoters in Coffea spp./
Author:	Gaitan, Alvaro Leon.
Description:	220 p.
Notes:	Source: Dissertation Abstracts International, Volume: 59-07, Section: B, page: 3148.
Contained By:	Dissertation Abstracts International59-07B.
Subject:	Agriculture, Plant Culture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9900076
ISBN:	0591969815

Cloning and evaluation of gene promoters in Coffea spp.
Gaitan, Alvaro Leon.

Cloning and evaluation of gene promoters in Coffea spp. - 220 p.

Source: Dissertation Abstracts International, Volume: 59-07, Section: B, page: 3148.

Thesis (Ph.D.)--Cornell University, 1998.

Coffee is the most important agricultural commodity in Colombia, and its cultivation is one of the most important industries in the country. Genetic engineering offers promising new ways to support traditional breeding programs in developing new coffee cultivars. This research is intended to initiate the study and evaluation of gene promoters in coffee as part of a biotechnology program carried out at the Colombian National Center of Coffee Research (CENICAFE). Promoters are an important part of the plant transformation system since they control the expression of transgenes.

ISBN: 0591969815Subjects--Topical Terms:

1018669
Agriculture, Plant Culture.

Cloning and evaluation of gene promoters in Coffea spp.
LDR:03284nmm 2200337 4500 001 1840725
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020 $a 0591969815
035 $a (UnM)AAI9900076
035 $a AAI9900076
040 $a UnM $c UnM
100 1 $a Gaitan, Alvaro Leon. $3 1929050
245 1 0 $a Cloning and evaluation of gene promoters in Coffea spp.
300 $a 220 p.
500 $a Source: Dissertation Abstracts International, Volume: 59-07, Section: B, page: 3148.
500 $a Adviser: Herb Aldwinckle.
502 $a Thesis (Ph.D.)--Cornell University, 1998.
520 $a Coffee is the most important agricultural commodity in Colombia, and its cultivation is one of the most important industries in the country. Genetic engineering offers promising new ways to support traditional breeding programs in developing new coffee cultivars. This research is intended to initiate the study and evaluation of gene promoters in coffee as part of a biotechnology program carried out at the Colombian National Center of Coffee Research (CENICAFE). Promoters are an important part of the plant transformation system since they control the expression of transgenes.
520 $a First, constitutive and defense-inducible gene sequences present in the GenBank were compared in order to determine the degree of homology among them. Only exon sequences presented similarities among the plant species studied, while promoter and intron sequences did not show significant similarities. This initial result suggested that promoters in Coffea spp. contain novel DNA sequences.
520 $a Second, PCR was explored as an alternative to genomic libraries in order to clone promoter sequences. For this, it was necessary clone the 5 $\ sp\prime $ end of the coding regions of the PAL and $\ alpha $ tubulin genes of the Timor Hybrid, using a modified "Touchdown" PCR with primers from consensus regions.
520 $a Third, these cloned regions were used as probes to identify positive PCR products from a technique known as Unpredictably Primed PCR (UP-PCR). The putative promoter region of the $\ alpha $ tubulin gene of the Timor Hybrid was cloned, and as expected, it did not show any similarity to corresponding promoters in other plants. A putative PAL promoter from C. arabica was cloned by screening a genomic library, and it turned out to be a novel sequence as well.
520 $a Fourth, constructs were made with different promoter sequences controlling the uidA gene, and transient expression was observed using particle bombardment on tobacco leaves and cell cultures, as well as on somatic embryos and tissue culture seedlings of Coffea arabica cv Colombia. Quantification of promoter strength was done in PEG transformed tobacco protoplasts.
520 $a Finally, transgenic tobacco plants were generated using Agrobacterium tumefaciens, and GUS evaluation results indicate that the coffee $\ alpha $ tubulin promoter activity was about 5 to 6 times lower than the activity of the double 35SCaMV.
590 $a School code: 0058.
650 4 $a Agriculture, Plant Culture. $3 1018669
650 4 $a Biology, Molecular. $3 1017719
690 $a 0479
690 $a 0307
710 2 0 $a Cornell University. $3 530586
773 0 $t Dissertation Abstracts International $g 59-07B.
790 1 0 $a Aldwinckle, Herb, $e advisor
790 $a 0058
791 $a Ph.D.
792 $a 1998
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9900076