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Molecular pathway identification usi...

Tress, Matthew David.

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Molecular pathway identification using microarray technology.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Molecular pathway identification using microarray technology./
Author:	Tress, Matthew David.
Description:	60 p.
Notes:	Source: Masters Abstracts International, Volume: 43-01, page: 0145.
Contained By:	Masters Abstracts International43-01.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1420981
ISBN:	0496257266

Molecular pathway identification using microarray technology.
Tress, Matthew David.

Molecular pathway identification using microarray technology. - 60 p.

Source: Masters Abstracts International, Volume: 43-01, page: 0145.

Thesis (M.S.)--Florida Atlantic University, 2004.

Harnessing the human genome using bioinformatics lead to the discovery of a highly cancer-selective gene, Single Minded 2 gene (SIM2). An isoform of the SIM2 gene, the short-form (SIM2-s), was shown to be specific to colon, pancreas, and prostate tumors. Antisense inhibition of SIM2-s in a colon carcinoma derived cell line (RKO) caused inhibition of gene expression, growth inhibition and apoptosis in vitro and in nude mice tumorigenicity models. To understand the mechanism of Sim2-s antisense, the antisense treated RKO colon cancer cells were monitored for genome wide expression using Affymetrix GeneChipRTM technology. A list of apoptosis related genes was generated using GeneSpringRTM software. Select GeneChip RTM output was validated by Quantitative RT-PCR. Relevance of a key gene, Growth arrest and DNA damage inducible (GADD45a), in the SIM2-s pathway was established. These results will provide a basis for the future experiments to understand the mechanism underlying Sim2-s activation in specific tumors.

ISBN: 0496257266Subjects--Topical Terms:

1017686
Biology, Cell.

Molecular pathway identification using microarray technology.
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245 1 0 $a Molecular pathway identification using microarray technology.
300 $a 60 p.
500 $a Source: Masters Abstracts International, Volume: 43-01, page: 0145.
500 $a Adviser: Ramaswamy Narayanan.
502 $a Thesis (M.S.)--Florida Atlantic University, 2004.
520 $a Harnessing the human genome using bioinformatics lead to the discovery of a highly cancer-selective gene, Single Minded 2 gene (SIM2). An isoform of the SIM2 gene, the short-form (SIM2-s), was shown to be specific to colon, pancreas, and prostate tumors. Antisense inhibition of SIM2-s in a colon carcinoma derived cell line (RKO) caused inhibition of gene expression, growth inhibition and apoptosis in vitro and in nude mice tumorigenicity models. To understand the mechanism of Sim2-s antisense, the antisense treated RKO colon cancer cells were monitored for genome wide expression using Affymetrix GeneChipRTM technology. A list of apoptosis related genes was generated using GeneSpringRTM software. Select GeneChip RTM output was validated by Quantitative RT-PCR. Relevance of a key gene, Growth arrest and DNA damage inducible (GADD45a), in the SIM2-s pathway was established. These results will provide a basis for the future experiments to understand the mechanism underlying Sim2-s activation in specific tumors.
590 $a School code: 0119.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
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