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Detection, characterization, and dis...

Nava, Alba Ruth.

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Detection, characterization, and distribution of begomoviruses infecting tomatoes in Venezuela.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Detection, characterization, and distribution of begomoviruses infecting tomatoes in Venezuela./
Author:	Nava, Alba Ruth.
Description:	120 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-03, Section: B, page: 1089.
Contained By:	Dissertation Abstracts International65-03B.
Subject:	Agriculture, Plant Pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3127676
ISBN:	0496749402

Detection, characterization, and distribution of begomoviruses infecting tomatoes in Venezuela.
Nava, Alba Ruth.

Detection, characterization, and distribution of begomoviruses infecting tomatoes in Venezuela. - 120 p.

Source: Dissertation Abstracts International, Volume: 65-03, Section: B, page: 1089.

Thesis (Ph.D.)--University of Florida, 2003.

Begomoviruses are members of the geminiviridae family. They are mono- or bipartite single-stranded DNA plant viruses that are transmitted by whiteflies. This genus belongs to the geminivirudae family. Begomoviruses are a limiting factor in the production of vegetables and other crops in the tropics and subtropics worldwide. To identify begomoviruses in tomatoes in Venezuela, symptomatic leaves were collected in ten states from 1993 to 1998. Detection of Begomovirus was performed by polymerase chain reaction (PCR) with four sets of primers. Begomoviruses were detected in 50% of the samples. Samples from Andean states (Trujillo, Merida, and Tachira) were selected to examine variability of begomovirus. Resulting sequences were placed in four groups based on BLAST, GAP, and phylogenetic analyses. Two groups were considered new begomoviruses based on low (<89%) nucleotide sequence identity and phylogenetic analyses. The viruses were designated 2.9-v (from Trujillo) and 57-v virus (from MErida). The full-length sequences of the A and B component of both viruses were obtained and compared with known begomoviruses. The 2.9v virus was closely related to Dicliptera yellow mottle virus (DiYMoV). However, the 57-v virus was a unique virus, distantly, but most closely related, to DiYMoV. The hypervariable regions of the B components of both viruses were used as specific probes to determine the distribution of these viruses. The 2.9-v virus was detected in samples from Trujillo, Lara, and Zulia. The 57-v virus was detected in samples from Merida, Guarico, and Aragua. The distribution of these viruses in such distant states could be explained by movement of infected transplants or by the whitefly vector. We confirmed the high variability of begomoviruses in the Andean states. We expect to find more distinct begomoviruses in samples from other states included in the survey. The genomic characterization of these two new begomoviruses and the generation of specific probes will allow the monitoring of the current prevalence of these two begomoviruses in tomato commercial areas of Venezuela. This information will also be used to develop a virus resistance program against these new begomoviruses using genetic engineering approaches.

ISBN: 0496749402Subjects--Topical Terms:

1028950
Agriculture, Plant Pathology.

Detection, characterization, and distribution of begomoviruses infecting tomatoes in Venezuela.
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100 1 $a Nava, Alba Ruth. $3 1927953
245 1 0 $a Detection, characterization, and distribution of begomoviruses infecting tomatoes in Venezuela.
300 $a 120 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-03, Section: B, page: 1089.
500 $a Chair: Jane E. Polston.
502 $a Thesis (Ph.D.)--University of Florida, 2003.
520 $a Begomoviruses are members of the geminiviridae family. They are mono- or bipartite single-stranded DNA plant viruses that are transmitted by whiteflies. This genus belongs to the geminivirudae family. Begomoviruses are a limiting factor in the production of vegetables and other crops in the tropics and subtropics worldwide. To identify begomoviruses in tomatoes in Venezuela, symptomatic leaves were collected in ten states from 1993 to 1998. Detection of Begomovirus was performed by polymerase chain reaction (PCR) with four sets of primers. Begomoviruses were detected in 50% of the samples. Samples from Andean states (Trujillo, Merida, and Tachira) were selected to examine variability of begomovirus. Resulting sequences were placed in four groups based on BLAST, GAP, and phylogenetic analyses. Two groups were considered new begomoviruses based on low (<89%) nucleotide sequence identity and phylogenetic analyses. The viruses were designated 2.9-v (from Trujillo) and 57-v virus (from MErida). The full-length sequences of the A and B component of both viruses were obtained and compared with known begomoviruses. The 2.9v virus was closely related to Dicliptera yellow mottle virus (DiYMoV). However, the 57-v virus was a unique virus, distantly, but most closely related, to DiYMoV. The hypervariable regions of the B components of both viruses were used as specific probes to determine the distribution of these viruses. The 2.9-v virus was detected in samples from Trujillo, Lara, and Zulia. The 57-v virus was detected in samples from Merida, Guarico, and Aragua. The distribution of these viruses in such distant states could be explained by movement of infected transplants or by the whitefly vector. We confirmed the high variability of begomoviruses in the Andean states. We expect to find more distinct begomoviruses in samples from other states included in the survey. The genomic characterization of these two new begomoviruses and the generation of specific probes will allow the monitoring of the current prevalence of these two begomoviruses in tomato commercial areas of Venezuela. This information will also be used to develop a virus resistance program against these new begomoviruses using genetic engineering approaches.
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