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Purification of zein and xanthophyll...

Kale, Aniket.

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Purification of zein and xanthophylls from corn by process chromatography.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Purification of zein and xanthophylls from corn by process chromatography./
作者:	Kale, Aniket.
面頁冊數:	150 p.
附註:	Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6128.
Contained By:	Dissertation Abstracts International67-11B.
標題:	Agriculture, Food Science and Technology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3242886
ISBN:	9780542988837

Purification of zein and xanthophylls from corn by process chromatography.
Kale, Aniket.

Purification of zein and xanthophylls from corn by process chromatography. - 150 p.

Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6128.

Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006.

The overall objective was to develop high-value corn-based coproducts to enhance the economic viability of the dry-grind ethanol industry. Two coproducts were identified: zero, an underutilized alcohol-soluble protein with a long history of study and a multitude of potential uses, and xanthophylls (lutein and zeaxanthin) which are oxygenated carotenoids that have numerous health benefits. Together, they have the potential to almost double the income of a typical dry-grind ethanol plant, provided methods can be developed to extract and purify these compounds.

ISBN: 9780542988837Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Purification of zein and xanthophylls from corn by process chromatography.
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100 1 $a Kale, Aniket. $3 1923607
245 1 0 $a Purification of zein and xanthophylls from corn by process chromatography.
300 $a 150 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6128.
500 $a Adviser: Munir Cheryan.
502 $a Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2006.
520 $a The overall objective was to develop high-value corn-based coproducts to enhance the economic viability of the dry-grind ethanol industry. Two coproducts were identified: zero, an underutilized alcohol-soluble protein with a long history of study and a multitude of potential uses, and xanthophylls (lutein and zeaxanthin) which are oxygenated carotenoids that have numerous health benefits. Together, they have the potential to almost double the income of a typical dry-grind ethanol plant, provided methods can be developed to extract and purify these compounds.
520 $a Chromatography was selected as the best method to separate and purify zein and xanthophylls, particularly size exclusion chromatography (SEC) due to the large differences in molecular size between zein, xanthophylls and the impurities. Since this technology would be incorporated in a dry-grind ethanol plant, aqueous ethanol was the preferred solvent for the entire processing chain, from the initial extraction of whole corn ground to the final purification steps. Three commercial resins (carbohydrate-based Sephadex LH20 and Superdex SP30, and methacrylate-based HW40) were chosen based on their ethanol stability and rated molecular cutoff of 5000 Daltons. These resins were characterized for selectivity, fractionation range, pore size distribution and surface morphology using scanning electron and atomic force microscopy with a view to identifying structure-function relationships that could also be useful in designing better stationary phases for specific applications. Under optimum conditions, the LH20 and SP30 resins satisfactorily separated zein and xanthophylls (as a group) from the impurities. These resins displayed some nonideal behavior due to interactions between the resin and the solutes. The LH20 showed an affinity for xanthophylls which facilitated their separation thereby resulting in a purer xanthophylls fraction. The SP30 showed an affinity for zein which would help to enhance the purity of zein.
520 $a Lower mobile phase polarity resulted in faster elution of xanthophylls as a group. On the other hand, higher polarities caused the impurities fraction to split into more peaks. The interactions between the impurities and zein may be ionic in nature while zein-xanthophylls binding is probably hydrophobic with negligible ionic or hydrogen bonding effects. The best solvent for the separation is 70% ethanol in terms of resolution and product purity.
520 $a Design parameters were optimized with two objective functions: maximizing zein throughput while maintaining the required resolution of xanthophylls and impurities. An increase in column height enhanced overall resolution. If pure xanthophylls devoid of impurities were the desired product, then longer columns should be used. There was little or no effect on resolution with an increase in column diameter indicating that the process is linearly scalable. An increase in temperature enhances the resolution slightly by improving mass transfer characteristics, but the upper temperature is restricted by the resin (40°C for the LH20). The maximum flow rate is restricted by the loss in resolution and high pressure drops. An increase in volume loading retarded elution of xanthophylls thus improving the xanthophylls-impurities separation but this would also result in higher cycle times. Mass loading did not affect the separation of impurities and xanthophylls.
520 $a The fractions eluting from the size exclusion column are dilute and need to be concentrated to reduce load on downstream operations and to recover the solvent. Adsorption chromatography (AC), though not suitable for separation or purification, could be used to concentrate the fractions. Membrane technology (nanofiltration or reverse osmosis) was also studied and found to be technically feasible.
520 $a A preliminary process design and economic analysis of the process suggested good economic returns by combining SEC and AC. About 80% of the capital cost and 85% of the operating cost of the process, as it stands now, is due only to the stationary phase. Our studies on the characteristics of the resins could be used to develop alternate lower-cost materials. In addition, the composition of the "impurities" fraction is unknown. There may be high-value compounds within that fraction that could further enhance the economic viability of the process.
590 $a School code: 0090.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
690 $a 0359
710 2 0 $a University of Illinois at Urbana-Champaign. $3 626646
773 0 $t Dissertation Abstracts International $g 67-11B.
790 1 0 $a Cheryan, Munir, $e advisor
790 $a 0090
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3242886