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The importance of sorting calcium in...

Li, Xiyan.

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The importance of sorting calcium in plant cells: Uncovering the roles of a sarcoplasmic/endoplasmic reticulum-like calcium ATPase.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The importance of sorting calcium in plant cells: Uncovering the roles of a sarcoplasmic/endoplasmic reticulum-like calcium ATPase./
Author:	Li, Xiyan.
Description:	234 p.
Notes:	Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6237.
Contained By:	Dissertation Abstracts International67-11B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3241498
ISBN:	9780542961823

The importance of sorting calcium in plant cells: Uncovering the roles of a sarcoplasmic/endoplasmic reticulum-like calcium ATPase.
Li, Xiyan.

The importance of sorting calcium in plant cells: Uncovering the roles of a sarcoplasmic/endoplasmic reticulum-like calcium ATPase. - 234 p.

Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6237.

Thesis (Ph.D.)--University of Maryland, College Park, 2006.

The spatial and temporal dynamics of intracellular Ca 2+ in response to environmental and hormonal cues underscore the importance of Ca2+ transport during plant growth and development. The Arabidopsis thaliana genome predicts multiple genes encoding Ca 2+ transporters, though the biological roles of most are unknown. Here I determine the function of AtECA3 which represents the first plant P-type 2A ATPase resembling mammalian s&barbelow;arcoplasmic/e&barbelow;ndoplasmic r&barbelow;eticulum C&barbelow;a ATPase (SERCA).

ISBN: 9780542961823Subjects--Topical Terms:

1017719
Biology, Molecular.

The importance of sorting calcium in plant cells: Uncovering the roles of a sarcoplasmic/endoplasmic reticulum-like calcium ATPase.
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035 $a (UMI)AAI3241498
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040 $a UMI $c UMI
100 1 $a Li, Xiyan. $3 1922852
245 1 4 $a The importance of sorting calcium in plant cells: Uncovering the roles of a sarcoplasmic/endoplasmic reticulum-like calcium ATPase.
300 $a 234 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6237.
500 $a Adviser: Heven Sze.
502 $a Thesis (Ph.D.)--University of Maryland, College Park, 2006.
520 $a The spatial and temporal dynamics of intracellular Ca 2+ in response to environmental and hormonal cues underscore the importance of Ca2+ transport during plant growth and development. The Arabidopsis thaliana genome predicts multiple genes encoding Ca 2+ transporters, though the biological roles of most are unknown. Here I determine the function of AtECA3 which represents the first plant P-type 2A ATPase resembling mammalian s&barbelow;arcoplasmic/e&barbelow;ndoplasmic r&barbelow;eticulum C&barbelow;a ATPase (SERCA).
520 $a AtECA3 (At1g10130) expressed in a yeast mutant lacking its endogenous Ca2+ pumps functionally substitutes for the defective Ca 2+-ATPases. AtECA3-dependent yeast growth is blocked by thapsigargin, a specific SERCA inhibitor. The results suggest that AtECA3 is a cation pump with specificity for Ca2+ and Mn2+, and that AtECA3 enhances yeast growth on Ca2+-depleted medium or on medium with high Mn2+ by sequestrating Ca2+ or Mn2+, respectively, into endomembrane compartments. AtECA3 is expressed in pollen grains as revealed by promoter::GUS analyses and a green fluorescence protein (GFP)-tagged to AtECA3 labels endomembranes at the pollen tip. In vitro tube growth of wild-type pollen is enhanced by 10 mM Ca2+, and inhibited by thapsigargin, suggesting that AtECA3 supports tube elongation by sorting intracellular Ca2+ to appropriate compartments. This idea is supported by genetic evidence, where three T-DNA insertional mutants show 33% reduction in pollen tube length. This defect lowers sperm transmission shown as segregation distortion and decreased seed set.
520 $a AtECA3 is also expressed in vascular tissues of young roots and leaves, and the GFP-tagged protein colocalizes with two Golgi markers. Three millimolar Ca2+ stimulate root growth of wild-type but not of mutants, indicating that Ca2+ accumulation in Golgi lumen is critical for growth. Root growth of eca3-4, but not of wild-type, is hypersensitive to 50 muM Mn2+. Thus loading Mn 2+ into Golgi lumen by AtECA3 supports root growth. Intriguingly, mutant roots show 80% increase in apoplastic peroxidase suggesting that secretory activities became deregulated.
520 $a In conclusion, I provide molecular evidence for a Golgi Ca2+/Mn 2+ pump in plants. Ca2+ and Mn2+ accumulation into Golgi/secretory compartments by AtECA3 and perhaps cation release from these stores affect secretory activities critical for root growth, pollen tube elongation and male fertility.
590 $a School code: 0117.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Plant Physiology. $3 1017865
690 $a 0307
690 $a 0369
690 $a 0817
710 2 0 $a University of Maryland, College Park. $3 657686
773 0 $t Dissertation Abstracts International $g 67-11B.
790 1 0 $a Sze, Heven, $e advisor
790 $a 0117
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3241498