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Implementation and evaluation of a m...

Codrea, Felicia.

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Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics./
作者:	Codrea, Felicia.
面頁冊數:	109 p.
附註:	Source: Masters Abstracts International, Volume: 44-06, page: 2800.
Contained By:	Masters Abstracts International44-06.
標題:	Chemistry, Biochemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1434339
ISBN:	9780542678820

Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics.
Codrea, Felicia.

Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics. - 109 p.

Source: Masters Abstracts International, Volume: 44-06, page: 2800.

Thesis (M.S.)--Michigan State University, 2006.

Although "gene therapy" is a concept that was introduced more than there decades ago, the successes of this method are below the expectations. The bottleneck of gene therapy is gene delivery. The current non-viral methods used for transfection possess low transfection efficiency and are not suitable for therapeutic use, showing high toxicity.

ISBN: 9780542678820Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics.
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245 1 0 $a Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics.
300 $a 109 p.
500 $a Source: Masters Abstracts International, Volume: 44-06, page: 2800.
500 $a Adviser: Rawle I. Hollingsworth.
502 $a Thesis (M.S.)--Michigan State University, 2006.
520 $a Although "gene therapy" is a concept that was introduced more than there decades ago, the successes of this method are below the expectations. The bottleneck of gene therapy is gene delivery. The current non-viral methods used for transfection possess low transfection efficiency and are not suitable for therapeutic use, showing high toxicity.
520 $a A new macromolecular scaffold that combines the properties of cationic polymers and cationic lipids was synthesized in only two steps. It contains a lipophilic part, like lipids, and a charged amine moiety, like cationic polymers. A polymeric backbone was synthesized through a Michael addition reaction, starting from 2(5H)-furanone and cysteine. After purification through membrane fractionation, the backbone was functionalized with N, N dimethylethylenediamine and octylamine. The ability of this new carrier to mediate transfection of plasmid encoding for green fluorescent protein in vitro was tested on COS 1 cells. The transfection conditions were optimized and an efficiency of transfection of 6.59% was achieved. The evaluation of the cytotoxicity of this new carrier proved that the carrier is non-toxic. The cells proliferated up to confluence even when the concentration was ten times higher than what was identified as optimum for transfection.
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650 4 $a Chemistry, Pharmaceutical. $3 550957
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