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Analysis of microbial communities in...

Wier, Andrew M.

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Analysis of microbial communities in hydrothermal vents in Yellowstone Lake, Yellowstone National Park, using 16S rDNA, functional metabolic genes and enrichment culture methods.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Analysis of microbial communities in hydrothermal vents in Yellowstone Lake, Yellowstone National Park, using 16S rDNA, functional metabolic genes and enrichment culture methods./
Author:	Wier, Andrew M.
Description:	108 p.
Notes:	Source: Dissertation Abstracts International, Volume: 67-06, Section: B, page: 2958.
Contained By:	Dissertation Abstracts International67-06B.
Subject:	Biology, Ecology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3222409
ISBN:	9780542750809

Analysis of microbial communities in hydrothermal vents in Yellowstone Lake, Yellowstone National Park, using 16S rDNA, functional metabolic genes and enrichment culture methods.
Wier, Andrew M.

Analysis of microbial communities in hydrothermal vents in Yellowstone Lake, Yellowstone National Park, using 16S rDNA, functional metabolic genes and enrichment culture methods. - 108 p.

Source: Dissertation Abstracts International, Volume: 67-06, Section: B, page: 2958.

Thesis (Ph.D.)--The University of Wisconsin - Milwaukee, 2006.

In this study of microbial communities in the hydrothermal vent system of Yellowstone Lake, WY, 56 clones of 16S rDNA revealed a great diversity of bacteria. Beneath Yellowstone Lake are hundreds of submerged hydrothermal vents that rise from the deep subsurface. These vents emit reduced inorganic compounds such as methane and hydrogen sulfide, which originate from volcanically heated strata deep below subsurface bedrock. To assess the bacterial communities present in these distinct habitats a small subunit ribosomal RNA sequence approach was used on three vent sites: Mary Bay vent, Otter vent, and West Thumb vent, and from thiosulfate enrichments inoculated with Mary Bay vent water. Sequence analysis showed the presence of organisms from 13 divisions in the bacterial domain. Many of the phylotypes from Mary Bay vent and Otter vent grouped with sulfide-oxidizing chemolithotrophs such as Thiobacillus and Thiovulum. The most abundant group of clones from West Thumb grouped with the hydrogen-oxidizing obligate chemolithotroph Aquifex. The most abundant phylotypes from the Mary Bay thiosulfate enrichments grouped with sulfide-oxidizers (Thiobacillus). The many cloned sequence affiliations with bacteria that oxidize inorganic compounds for CO2 fixation and the vent chemistry data indicate chemoautotrophic bacterial metabolism in these freshwater vent communities.

ISBN: 9780542750809Subjects--Topical Terms:

1017726
Biology, Ecology.

Analysis of microbial communities in hydrothermal vents in Yellowstone Lake, Yellowstone National Park, using 16S rDNA, functional metabolic genes and enrichment culture methods.
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100 1 $a Wier, Andrew M. $3 1919104
245 1 0 $a Analysis of microbial communities in hydrothermal vents in Yellowstone Lake, Yellowstone National Park, using 16S rDNA, functional metabolic genes and enrichment culture methods.
300 $a 108 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-06, Section: B, page: 2958.
500 $a Adviser: Charles F. Wimpee.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Milwaukee, 2006.
520 $a In this study of microbial communities in the hydrothermal vent system of Yellowstone Lake, WY, 56 clones of 16S rDNA revealed a great diversity of bacteria. Beneath Yellowstone Lake are hundreds of submerged hydrothermal vents that rise from the deep subsurface. These vents emit reduced inorganic compounds such as methane and hydrogen sulfide, which originate from volcanically heated strata deep below subsurface bedrock. To assess the bacterial communities present in these distinct habitats a small subunit ribosomal RNA sequence approach was used on three vent sites: Mary Bay vent, Otter vent, and West Thumb vent, and from thiosulfate enrichments inoculated with Mary Bay vent water. Sequence analysis showed the presence of organisms from 13 divisions in the bacterial domain. Many of the phylotypes from Mary Bay vent and Otter vent grouped with sulfide-oxidizing chemolithotrophs such as Thiobacillus and Thiovulum. The most abundant group of clones from West Thumb grouped with the hydrogen-oxidizing obligate chemolithotroph Aquifex. The most abundant phylotypes from the Mary Bay thiosulfate enrichments grouped with sulfide-oxidizers (Thiobacillus). The many cloned sequence affiliations with bacteria that oxidize inorganic compounds for CO2 fixation and the vent chemistry data indicate chemoautotrophic bacterial metabolism in these freshwater vent communities.
520 $a Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) partial gene sequences were amplified from samples collected from three hydrothermal vents in Yellowstone Lake. The form I gene, cbbL, was found in two vent samples, but form II, cbbM, was not found in any samples. One set of RuBisCo clone sequences (4 phylotypes) grouped within the cyanobacterial IA clade that contains two marine genera, Prochlorococcus marinus and Synechococcus sp. This is the first report of cyanobacterial-like form IA RuBisCo found in a freshwater environment. Other form IA sequences grouped with photoheterotrophic bacterial genera Rhodobacter and Nitrobacter. Clones from the form IB clade fell into two groups, one with Thermosynechococcus and Prochlorothrix and another with Synechococcus OH28. In this study, bacterial cbbL sequences suggest that diverse chemoautotrophs may contribute significantly to CO2 fixation within this freshwater hydrothermal vent system.
590 $a School code: 0263.
650 4 $a Biology, Ecology. $3 1017726
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0329
690 $a 0410
710 2 0 $a The University of Wisconsin - Milwaukee. $3 1019345
773 0 $t Dissertation Abstracts International $g 67-06B.
790 1 0 $a Wimpee, Charles F., $e advisor
790 $a 0263
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3222409