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Cloning of RCY1 and characterization...

Soosaar, Jennifer Miller.

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Cloning of RCY1 and characterization of its role in cucumber mosaic virus resistance in Arabidopsis thaliana.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Cloning of RCY1 and characterization of its role in cucumber mosaic virus resistance in Arabidopsis thaliana./
作者:	Soosaar, Jennifer Miller.
面頁冊數:	154 p.
附註:	Source: Dissertation Abstracts International, Volume: 67-04, Section: B, page: 1838.
Contained By:	Dissertation Abstracts International67-04B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3214306
ISBN:	9780542653803

Cloning of RCY1 and characterization of its role in cucumber mosaic virus resistance in Arabidopsis thaliana.
Soosaar, Jennifer Miller.

Cloning of RCY1 and characterization of its role in cucumber mosaic virus resistance in Arabidopsis thaliana. - 154 p.

Source: Dissertation Abstracts International, Volume: 67-04, Section: B, page: 1838.

Thesis (Ph.D.)--Yale University, 2006.

Cucumber mosaic virus (CMV) is a devastating crop pathogen with a broad host range at greater than 1200 species. Understanding natural forms of resistance to this pathogen will enable the generation of crops with genetic resistance. The Yellow strain of CMV infects Arabidopsis thaliana, but is unable to replicate and spread in the A. thaliana ecotype C24. I mapped and cloned in C24 the Resistance (R) gene RCY1, a single dominant gene that confers CMV-Y resistance. RCY1 is the third functional allele of this resistance locus with each protein conferring resistance to an unrelated pathogen. The proteins encoded by these alleles have putatively undergone positive selection, resulting in this altered pathogen specificity. RCY1 contains a nucleotide binding site and a leucine-rich repeat domain, domains conserved in innate immunity in mammals and Drosophila. The conservation of these domains between organisms that diverged millennia ago indicates that R gene-mediated innate immunity is an ancient defense mechanism.

ISBN: 9780542653803Subjects--Topical Terms:

1017719
Biology, Molecular.

Cloning of RCY1 and characterization of its role in cucumber mosaic virus resistance in Arabidopsis thaliana.
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520 $a Cucumber mosaic virus (CMV) is a devastating crop pathogen with a broad host range at greater than 1200 species. Understanding natural forms of resistance to this pathogen will enable the generation of crops with genetic resistance. The Yellow strain of CMV infects Arabidopsis thaliana, but is unable to replicate and spread in the A. thaliana ecotype C24. I mapped and cloned in C24 the Resistance (R) gene RCY1, a single dominant gene that confers CMV-Y resistance. RCY1 is the third functional allele of this resistance locus with each protein conferring resistance to an unrelated pathogen. The proteins encoded by these alleles have putatively undergone positive selection, resulting in this altered pathogen specificity. RCY1 contains a nucleotide binding site and a leucine-rich repeat domain, domains conserved in innate immunity in mammals and Drosophila. The conservation of these domains between organisms that diverged millennia ago indicates that R gene-mediated innate immunity is an ancient defense mechanism.
520 $a Upon recognition of a pathogen, defense signaling is transduced through hormones. By crossing hormone signaling mutants to C24, I determined that the signaling hormones salicylic acid and ethylene are required for RCY1-mediated resistance to CMV-Y, while jasmonic acid is not. I also performed yeast two-hybrid screens with different domains of RCY1 in order to identify proteins that may be necessary to mount a defense response to CMV-Y. R&barbelow;CY1 interacting aspartic p&barbelow;rotease, RASP, was isolated through this screen. RASP and RCY1 colocalize to the endoplasmic reticulum of plant cells. Upon transgenic overexpression of RASP in C24, resistance to CMV-Y is lost, and the virus replicates and spreads through out the plant. Knock-down of RASP expression has no affect on resistance. I propose that RASP is cleaving a positive regulator of RCY1-mediated defense signaling.
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