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THE REPRODUCTIVE BIOLOGY AND GAMETE ...

LYNN, JOHN WELTON.

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THE REPRODUCTIVE BIOLOGY AND GAMETE INTERACTION IN THE FRESHWATER PRAWN MACROBRACHIUM ROSENBERGII.

Record Type:	Electronic resources : Monograph/item
Title/Author:	THE REPRODUCTIVE BIOLOGY AND GAMETE INTERACTION IN THE FRESHWATER PRAWN MACROBRACHIUM ROSENBERGII./
Author:	LYNN, JOHN WELTON.
Description:	175 p.
Notes:	Source: Dissertation Abstracts International, Volume: 42-09, Section: B, page: 3545.
Contained By:	Dissertation Abstracts International42-09B.
Subject:	Biology, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8205014

THE REPRODUCTIVE BIOLOGY AND GAMETE INTERACTION IN THE FRESHWATER PRAWN MACROBRACHIUM ROSENBERGII.
LYNN, JOHN WELTON.

THE REPRODUCTIVE BIOLOGY AND GAMETE INTERACTION IN THE FRESHWATER PRAWN MACROBRACHIUM ROSENBERGII. - 175 p.

Source: Dissertation Abstracts International, Volume: 42-09, Section: B, page: 3545.

Thesis (Ph.D.)--University of California, Davis, 1981.

Nonflagellated sperm of M. rosenbergii resemble an inverted umbrella. A 10 (mu)m diameter main body (base) houses the nucleus and a single 12 (mu)m long spike extends from the convex surface of the base. Radial fibrils in the base anastomose to form the spike. These structures contain 6 nm filaments with a 32 nm cross-striated periodicity. Centrioles are closely associated with the radial fibrils at the spike base. Eggs, covered by a bilayered investment coat composed of a 0.5 (mu)m protein outer layer and a 2.5 (mu)m mucopolysaccharide inner layer, are spawned through an externally held spermatophore following a female's ovigerous molt and mating. Sperm (< 5/egg) attach base-first with the spike oriented perpendicularly to the investment coat. Within 15 seconds, the spike bends at the base and contacts the investment coat. The spike penetrates the investment coat, the base is inverted, and fertilization occurs within 2 minutes. The spike remains briefly as a central core in a fertilization cone. Fertilization is not inhibited by cytochalasin B and sperm isolated with and without protease inhibitors have no actin-comigrating band on SDS polyacrylamide gel electrophoresis. Fluorescein-tagged DNAase I does not label the spike or radial fibrils. Spike bending can be induced in vitro with ATP or GTP treatment on glycerinated sperm. Investment coat penetration is inhibited by 0.01% soybean trypsin inhibitor or tosyl-L-lysine chloromethyl ketone but not by L-1-tosylamide-2-phenylethyl chloromethyl ketone. These data suggest spike bending is a nonactin contractile process and investment coat penetration is facilitated by a serine protease(s).Subjects--Topical Terms:

1018625
Biology, General.

THE REPRODUCTIVE BIOLOGY AND GAMETE INTERACTION IN THE FRESHWATER PRAWN MACROBRACHIUM ROSENBERGII.
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100 1 $a LYNN, JOHN WELTON. $3 1914665
245 1 4 $a THE REPRODUCTIVE BIOLOGY AND GAMETE INTERACTION IN THE FRESHWATER PRAWN MACROBRACHIUM ROSENBERGII.
300 $a 175 p.
500 $a Source: Dissertation Abstracts International, Volume: 42-09, Section: B, page: 3545.
502 $a Thesis (Ph.D.)--University of California, Davis, 1981.
520 $a Nonflagellated sperm of M. rosenbergii resemble an inverted umbrella. A 10 (mu)m diameter main body (base) houses the nucleus and a single 12 (mu)m long spike extends from the convex surface of the base. Radial fibrils in the base anastomose to form the spike. These structures contain 6 nm filaments with a 32 nm cross-striated periodicity. Centrioles are closely associated with the radial fibrils at the spike base. Eggs, covered by a bilayered investment coat composed of a 0.5 (mu)m protein outer layer and a 2.5 (mu)m mucopolysaccharide inner layer, are spawned through an externally held spermatophore following a female's ovigerous molt and mating. Sperm (< 5/egg) attach base-first with the spike oriented perpendicularly to the investment coat. Within 15 seconds, the spike bends at the base and contacts the investment coat. The spike penetrates the investment coat, the base is inverted, and fertilization occurs within 2 minutes. The spike remains briefly as a central core in a fertilization cone. Fertilization is not inhibited by cytochalasin B and sperm isolated with and without protease inhibitors have no actin-comigrating band on SDS polyacrylamide gel electrophoresis. Fluorescein-tagged DNAase I does not label the spike or radial fibrils. Spike bending can be induced in vitro with ATP or GTP treatment on glycerinated sperm. Investment coat penetration is inhibited by 0.01% soybean trypsin inhibitor or tosyl-L-lysine chloromethyl ketone but not by L-1-tosylamide-2-phenylethyl chloromethyl ketone. These data suggest spike bending is a nonactin contractile process and investment coat penetration is facilitated by a serine protease(s).
590 $a School code: 0029.
650 4 $a Biology, General. $3 1018625
690 $a 0306
710 2 0 $a University of California, Davis. $3 1018682
773 0 $t Dissertation Abstracts International $g 42-09B.
790 $a 0029
791 $a Ph.D.
792 $a 1981
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8205014