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Real-time polymerase chain reaction ...

Morgan, Karen Lynn.

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Real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis./
Author:	Morgan, Karen Lynn.
Description:	69 p.
Notes:	Source: Masters Abstracts International, Volume: 44-03, page: 1289.
Contained By:	Masters Abstracts International44-03.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1429440
ISBN:	9780542371097

Real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis.
Morgan, Karen Lynn.

Real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis. - 69 p.

Source: Masters Abstracts International, Volume: 44-03, page: 1289.

Thesis (M.S.)--San Jose State University, 2005.

The resurgence of tuberculosis is a serious public health issue in the United States and a leading cause of death worldwide. The causative agent, M. tuberculosis, is a slow growing bacterial pathogen. Consequently, diagnostic testing based on conventional culture techniques is delayed. The Center for Disease Control recommends nucleic acid amplification testing on clinical specimens to speed the diagnosis of tuberculosis, thereby enabling prompt treatment. This thesis describes the development of a real-time polymerase chain reaction (RT-PCR) assay which yields rapid, sensitive and specific detection of M. tuberculosis in clinical specimens. The RT-PCR assay was designed to target the ITS region of the 16S rRNA gene of M. tuberculosis using Taqman hybridization probes for detection of amplicons. Analyzing 231 respiratory specimens composed of 76 specimens from culture-confirmed tuberculosis cases and 155 culture negative specimens, the developed RT-PCR assay achieved a sensitivity of 85.5% and specificity of 100%.

ISBN: 9780542371097Subjects--Topical Terms:

1017734
Biology, Microbiology.

Real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis.
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100 1 $a Morgan, Karen Lynn. $3 1914311
245 1 0 $a Real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis.
300 $a 69 p.
500 $a Source: Masters Abstracts International, Volume: 44-03, page: 1289.
500 $a Adviser: William Murray.
502 $a Thesis (M.S.)--San Jose State University, 2005.
520 $a The resurgence of tuberculosis is a serious public health issue in the United States and a leading cause of death worldwide. The causative agent, M. tuberculosis, is a slow growing bacterial pathogen. Consequently, diagnostic testing based on conventional culture techniques is delayed. The Center for Disease Control recommends nucleic acid amplification testing on clinical specimens to speed the diagnosis of tuberculosis, thereby enabling prompt treatment. This thesis describes the development of a real-time polymerase chain reaction (RT-PCR) assay which yields rapid, sensitive and specific detection of M. tuberculosis in clinical specimens. The RT-PCR assay was designed to target the ITS region of the 16S rRNA gene of M. tuberculosis using Taqman hybridization probes for detection of amplicons. Analyzing 231 respiratory specimens composed of 76 specimens from culture-confirmed tuberculosis cases and 155 culture negative specimens, the developed RT-PCR assay achieved a sensitivity of 85.5% and specificity of 100%.
590 $a School code: 6265.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
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