東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

The effects of nutrient supply on de...

Sawyer, Linda Kay.

Linked to FindBook

Google Book

Amazon

博客來

The effects of nutrient supply on detachment rates of bacteria from solid surfaces.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The effects of nutrient supply on detachment rates of bacteria from solid surfaces./
Author:	Sawyer, Linda Kay.
Description:	231 p.
Notes:	Source: Dissertation Abstracts International, Volume: 60-05, Section: B, page: 2287.
Contained By:	Dissertation Abstracts International60-05B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9931388
ISBN:	9780599316058

The effects of nutrient supply on detachment rates of bacteria from solid surfaces.
Sawyer, Linda Kay.

The effects of nutrient supply on detachment rates of bacteria from solid surfaces. - 231 p.

Source: Dissertation Abstracts International, Volume: 60-05, Section: B, page: 2287.

Thesis (Ph.D.)--University of California, Berkeley, 1999.

Detachment of bacteria is a complex and poorly understood process. The objective of this work was to investigate the hypothesis that nutrient depletion at the surface leads to detachment of bacterial cells growing on that surface.

ISBN: 9780599316058Subjects--Topical Terms:

1017734
Biology, Microbiology.

The effects of nutrient supply on detachment rates of bacteria from solid surfaces.
LDR:03402nmm 2200361 4500 001 1825025
005 20061205094413.5
008 130610s1999 eng d
020 $a 9780599316058
035 $a (UnM)AAI9931388
035 $a AAI9931388
040 $a UnM $c UnM
100 1 $a Sawyer, Linda Kay. $3 1914050
245 1 4 $a The effects of nutrient supply on detachment rates of bacteria from solid surfaces.
300 $a 231 p.
500 $a Source: Dissertation Abstracts International, Volume: 60-05, Section: B, page: 2287.
500 $a Chair: Slawomir W. Hermanowicz.
502 $a Thesis (Ph.D.)--University of California, Berkeley, 1999.
520 $a Detachment of bacteria is a complex and poorly understood process. The objective of this work was to investigate the hypothesis that nutrient depletion at the surface leads to detachment of bacterial cells growing on that surface.
520 $a Two bacterial strains, Aeromonas hydrophila and Pseudomonas aeruginosa, were observed in a developing biofilm using phase contrast microscopy of glass parallel plate flow cells. The effects of a range of nutrient concentrations on the behavior of attached bacteria were investigated, and nutrient concentrations were abruptly changed to induce dynamic effects. Images were taken every 15 minutes, and image analysis was used to determine the specific growth rate and specific detachment rate.
520 $a Attached growth rates in most experiments were consistent with suspended results. However, after an abrupt concentration increase, attached growth rates were lower than predicted from suspended kinetics, probably due to a transient lag phase or increased nutrient depletion.
520 $a Accumulation rates were negative for extended periods when Aeromonas hydrophila was grown below 43 mg TOC/L or without casamino acids. Below 32 mg TOC/L in complex medium, organisms that did not detach immediately after the concentration change became inactive on the surface. Viability staining showed that bacteria did not respire after prolonged exposure to low concentrations of complex medium.
520 $a An observable parameter proportional to the nutrient depletion at the surface was used to indicate nutrient depletion. This parameter is proportional to the specific growth rate and the area of cells on the surface and inversely proportional to the bulk medium concentration and the number of colonies on the surface. Because the depletion parameter assumes an overall average growth rate for all the cells on the surface, in situations where growth rates vary widely between cells, as occurred during prolonged negative accumulations, the parameter is not valid.
520 $a Detachment was a function of depletion parameter before abrupt concentration changes in most cases, but the depletion parameter was not scaleable from one set of data to another.
520 $a After abrupt concentration changes, detachment rates were a function of growth rates. Different curves applied after an increase and decrease. The same relationship applied to all three organism-media combinations studied.
590 $a School code: 0028.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Engineering, Environmental. $3 783782
650 4 $a Engineering, Civil. $3 783781
690 $a 0410
690 $a 0775
690 $a 0543
710 2 0 $a University of California, Berkeley. $3 687832
773 0 $t Dissertation Abstracts International $g 60-05B.
790 1 0 $a Hermanowicz, Slawomir W., $e advisor
790 $a 0028
791 $a Ph.D.
792 $a 1999
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9931388