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Biologically modified diamond thin f...

Yang, Wensha.

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Biologically modified diamond thin film for biosensing applications.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Biologically modified diamond thin film for biosensing applications./
Author:	Yang, Wensha.
Description:	197 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2563.
Contained By:	Dissertation Abstracts International66-05B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3175438
ISBN:	9780542138409

Biologically modified diamond thin film for biosensing applications.
Yang, Wensha.

Biologically modified diamond thin film for biosensing applications. - 197 p.

Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2563.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2005.

Bioelectronics is a rapidly progressing field at the junction of chemistry, biochemistry, physics and materials science. For applications such as homeland security and environmental sensing, one goal is to integrate biomolecules with semiconducting materials to enable real-time, low power biosensing. Gold and silicon have been used previously to detect biological binding events. However neither of the materials above has shown sufficient stability in repetitive biological binding and continuous monitoring.

ISBN: 9780542138409Subjects--Topical Terms:

586156
Chemistry, Analytical.

Biologically modified diamond thin film for biosensing applications.
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100 1 $a Yang, Wensha. $3 1913940
245 1 0 $a Biologically modified diamond thin film for biosensing applications.
300 $a 197 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2563.
500 $a Supervisor: Robert J. Hamers.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2005.
520 $a Bioelectronics is a rapidly progressing field at the junction of chemistry, biochemistry, physics and materials science. For applications such as homeland security and environmental sensing, one goal is to integrate biomolecules with semiconducting materials to enable real-time, low power biosensing. Gold and silicon have been used previously to detect biological binding events. However neither of the materials above has shown sufficient stability in repetitive biological binding and continuous monitoring.
520 $a This work was aimed at using nanocrystalline thin film diamond surface as a substrate for biological modification, and in particular, using the chemical stability and electrical properties of diamond to develop biosensors that are able to achieve direct electronic sensing of biological binding events. The research in this work yields new understanding of how biological modification changes the electrical properties of the diamond surfaces, and an important step in the development of biosensors with high sensitivity.
520 $a Diamond surfaces were covalently tethered with organic monolayers via UV-mediated photochemical reactions to provide suitable functionalities for subsequent DNA attachment. The modified surfaces show excellent stability for repetitive DNA hybridization.
520 $a Electrochemical impedance spectroscopy (EIS) was used to investigate changes in interfacial electrical properties that arise when DNA modified diamond surfaces are exposed to solution-phase DNA oligonucleotides with complementary and non-complementary sequences. Exposure to complementary DNA sequence produced pronounced changes in impedance, while exposure to non-complementary DNA sequence only produced negligible responses. The electrical response is also sensitive to the doping concentration and doping type. Circuit modeling was used to understand the electrical signal induced by biological binding events. Field effect induced by molecular charges was identified to be the detection mechanism.
520 $a A biologically sensitive field effect transistor (FET) was fabricated using such a surface. Electrical measurements reveal behavior characteristic of field-effect transistors. The bio-FET device made on an IgG-modified diamond exhibits a response specific to the anti-IgG antibody.
520 $a Diazonium chemistry was used to modify diamond with molecules bearing nitro groups. Electrochemical reduction was then used to specifically reduce nitro groups to amine groups on the diamond coated electrode of interest. This method provides a pathway toward diamond arrays without the use of microfluidics.
590 $a School code: 0262.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Inorganic. $3 517253
650 4 $a Chemistry, Physical. $3 560527
690 $a 0486
690 $a 0488
690 $a 0494
710 2 0 $a The University of Wisconsin - Madison. $3 626640
773 0 $t Dissertation Abstracts International $g 66-05B.
790 1 0 $a Hamers, Robert J., $e advisor
790 $a 0262
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3175438