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Plant cell wall-degradation and twin...

Gonzalez, Enid T.

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Plant cell wall-degradation and twin-arginine translocation: Exploring Ralstonia solanacearum virulence factors.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Plant cell wall-degradation and twin-arginine translocation: Exploring Ralstonia solanacearum virulence factors./
Author:	Gonzalez, Enid T.
Description:	156 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2407.
Contained By:	Dissertation Abstracts International66-05B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3175389
ISBN:	9780542137914

Plant cell wall-degradation and twin-arginine translocation: Exploring Ralstonia solanacearum virulence factors.
Gonzalez, Enid T.

Plant cell wall-degradation and twin-arginine translocation: Exploring Ralstonia solanacearum virulence factors. - 156 p.

Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2407.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2005.

The bacterial wilt pathogen Ralstonia solanacearum relies on several secreted proteins to cause disease on its plant host. A suite of three plant cell wall degrading enzymes, polygalacturonases (PGs) (PehA, PehB, and PehC), were hypothesized to facilitate the entry and spread of the bacterium, and to generate a carbon source, galacturonic acid, from host cell walls. Until recently the collective contribution of these enzymes to disease had not been studied due to the lack of a fully non-pectolytic strain (pehApehBpehC-) of R. solanacearum. Unlike the individual pehA and pehB mutants, a pehC mutant was not reduced in virulence. Surprisingly, a pehA pehB pehC- strain of R. solanacearum was significantly more virulent than its pehA pehB- parent strain, yet less virulent than wild type. PehC may actually trigger plant host defenses by degrading oligogalacturonide signals into more potent elicitors; therefore, its absence could make the triple mutant strain less visible to its host and thus increase its virulence.

ISBN: 9780542137914Subjects--Topical Terms:

1017734
Biology, Microbiology.

Plant cell wall-degradation and twin-arginine translocation: Exploring Ralstonia solanacearum virulence factors.
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008 130610s2005 eng d
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035 $a (UnM)AAI3175389
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040 $a UnM $c UnM
100 1 $a Gonzalez, Enid T. $3 1913544
245 1 0 $a Plant cell wall-degradation and twin-arginine translocation: Exploring Ralstonia solanacearum virulence factors.
300 $a 156 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2407.
500 $a Supervisor: Caitilyn Allen.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2005.
520 $a The bacterial wilt pathogen Ralstonia solanacearum relies on several secreted proteins to cause disease on its plant host. A suite of three plant cell wall degrading enzymes, polygalacturonases (PGs) (PehA, PehB, and PehC), were hypothesized to facilitate the entry and spread of the bacterium, and to generate a carbon source, galacturonic acid, from host cell walls. Until recently the collective contribution of these enzymes to disease had not been studied due to the lack of a fully non-pectolytic strain (pehApehBpehC-) of R. solanacearum. Unlike the individual pehA and pehB mutants, a pehC mutant was not reduced in virulence. Surprisingly, a pehA pehB pehC- strain of R. solanacearum was significantly more virulent than its pehA pehB- parent strain, yet less virulent than wild type. PehC may actually trigger plant host defenses by degrading oligogalacturonide signals into more potent elicitors; therefore, its absence could make the triple mutant strain less visible to its host and thus increase its virulence.
520 $a The hypothesis that R. solanacearum PGs function to nourish bacteria inside of the plant by generating a usable carbon source in the form of galacturonic acid was tested by creating a mutant that was fully pectolytic, but could no longer uptake PG degradation products because it lacked exuT, a galacturonic acid transporter. An R. solanacearum exuT mutant could no longer uptake PG degradation products, but retained full wild type virulence. These data clearly demonstrated that nutrition generated by PG activity does not contribute to pathogenic fitness in tomato plants.
520 $a To identify novel R. solanacearum secreted virulence factors, the twin-arginine translocation (Tat) system was mutated by disrupting tatC, which encodes an indispensable component of Tat. An R. solanacearum tatC mutant had a pleiotropic phenotype and was significantly reduced in virulence; however, the individual contributors to virulence couldn't be identified. There are 70 predicted R. solanacearum Tat secreted proteins, and six were mutated and studied. pehC, nosZ; plcN; rsp1575, a periplasmic binding protein; and rsp1521, an AcvB-like protein involved in acid tolerance were all required for full bacterial wilt virulence. A mutant defective in rsc1651, a conserved hypothetical protein, had wild type virulence.
590 $a School code: 0262.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Agriculture, Plant Pathology. $3 1028950
690 $a 0410
690 $a 0480
710 2 0 $a The University of Wisconsin - Madison. $3 626640
773 0 $t Dissertation Abstracts International $g 66-05B.
790 1 0 $a Allen, Caitilyn, $e advisor
790 $a 0262
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3175389