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Recombination genes of the spirochet...

Driver, Adrienne Danielle.

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Recombination genes of the spirochete genus Borrelia.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Recombination genes of the spirochete genus Borrelia./
Author:	Driver, Adrienne Danielle.
Description:	138 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-01, Section: B, page: 0085.
Contained By:	Dissertation Abstracts International66-01B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3160705
ISBN:	9780496939534

Recombination genes of the spirochete genus Borrelia.
Driver, Adrienne Danielle.

Recombination genes of the spirochete genus Borrelia. - 138 p.

Source: Dissertation Abstracts International, Volume: 66-01, Section: B, page: 0085.

Thesis (Ph.D.)--University of California, Irvine, 2005.

These studies revealed that (1) the B. hermsii genome contained a gene encoding the homologous recombination protein RecA as well as genes encoding the homologous recombination mediators recB, recC, and recD, (2) the RecA proteins of B. burgdorferi and B. hermsii were functional homologs of the E. coli RecA protein, and (3) both recA and recB genes are expressed during growth in vitro and in vivo, and the expression levels of these genes were unaffected by changes in the environment. Although the gnome sequenc of B. hermsii is unknown, complete recA, recB, recC, recD and recG (Zhong et al. unpublished) ORFs have been identified, and hybridization of B. hermsii genomic DNA to B. burgdorferi microarrays have identified that B. hermsii contains sequences that hybridize to locations corresponding the Holliday junction helicase genes ruvA and ruvB {Zhong, 2004 #111}. The identification of theses genes provides the means for B. hermsii to carryout homologous-recombination mediated events that may include antigenic variation. Expression of both B. burgdorferi and B. hermsii RecA protein in RecA deficient E. coli cells restores homologous recombination as well as restoring recombinational DNA repair, although the ability of B. burgdorferi RecA protein to do so is significantly greater than B. hermsii RecA protein. Through site-directed mutagenesis I determined that a substitution at position 152 in the B. hermsii RecA protein (a Q for a K) was wholly or partially responsible for the reduced ability of B. hermsii RecA protein to complement the defective recombinational DNA repair system of E. coli. Finally using quantitative RT-PCR expression of recA and recB genes was detected from samples of B. hermsii grown under both in vitro culture conditions and in vivo in mice, and that expression levels were not effected by changes in cell density or the presence of an active immune response.

ISBN: 9780496939534Subjects--Topical Terms:

1017734
Biology, Microbiology.

Recombination genes of the spirochete genus Borrelia.
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100 1 $a Driver, Adrienne Danielle. $3 1912948
245 1 0 $a Recombination genes of the spirochete genus Borrelia.
300 $a 138 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-01, Section: B, page: 0085.
500 $a Chair: Alan G. Barbour.
502 $a Thesis (Ph.D.)--University of California, Irvine, 2005.
520 $a These studies revealed that (1) the B. hermsii genome contained a gene encoding the homologous recombination protein RecA as well as genes encoding the homologous recombination mediators recB, recC, and recD, (2) the RecA proteins of B. burgdorferi and B. hermsii were functional homologs of the E. coli RecA protein, and (3) both recA and recB genes are expressed during growth in vitro and in vivo, and the expression levels of these genes were unaffected by changes in the environment. Although the gnome sequenc of B. hermsii is unknown, complete recA, recB, recC, recD and recG (Zhong et al. unpublished) ORFs have been identified, and hybridization of B. hermsii genomic DNA to B. burgdorferi microarrays have identified that B. hermsii contains sequences that hybridize to locations corresponding the Holliday junction helicase genes ruvA and ruvB {Zhong, 2004 #111}. The identification of theses genes provides the means for B. hermsii to carryout homologous-recombination mediated events that may include antigenic variation. Expression of both B. burgdorferi and B. hermsii RecA protein in RecA deficient E. coli cells restores homologous recombination as well as restoring recombinational DNA repair, although the ability of B. burgdorferi RecA protein to do so is significantly greater than B. hermsii RecA protein. Through site-directed mutagenesis I determined that a substitution at position 152 in the B. hermsii RecA protein (a Q for a K) was wholly or partially responsible for the reduced ability of B. hermsii RecA protein to complement the defective recombinational DNA repair system of E. coli. Finally using quantitative RT-PCR expression of recA and recB genes was detected from samples of B. hermsii grown under both in vitro culture conditions and in vivo in mice, and that expression levels were not effected by changes in cell density or the presence of an active immune response.
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