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PARTIAL CHARACTERIZATION AND GROUPIN...

TAIWO, MONILOLA ARINOLA.

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PARTIAL CHARACTERIZATION AND GROUPING OF ISOLATES OF BLACKEYE COWPEA AND COWPEA APHID-BORNE MOSAIC VIRUSES; AND INHERITANCE OF RESISTANCE TO BLACKEYE COWPEA MOSAIC VIRUS.

Record Type:	Electronic resources : Monograph/item
Title/Author:	PARTIAL CHARACTERIZATION AND GROUPING OF ISOLATES OF BLACKEYE COWPEA AND COWPEA APHID-BORNE MOSAIC VIRUSES; AND INHERITANCE OF RESISTANCE TO BLACKEYE COWPEA MOSAIC VIRUS./
Author:	TAIWO, MONILOLA ARINOLA.
Description:	95 p.
Notes:	Source: Dissertation Abstracts International, Volume: 42-04, Section: B, page: 1255.
Contained By:	Dissertation Abstracts International42-04B.
Subject:	Agriculture, Plant Pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8119429

PARTIAL CHARACTERIZATION AND GROUPING OF ISOLATES OF BLACKEYE COWPEA AND COWPEA APHID-BORNE MOSAIC VIRUSES; AND INHERITANCE OF RESISTANCE TO BLACKEYE COWPEA MOSAIC VIRUS.
TAIWO, MONILOLA ARINOLA.

PARTIAL CHARACTERIZATION AND GROUPING OF ISOLATES OF BLACKEYE COWPEA AND COWPEA APHID-BORNE MOSAIC VIRUSES; AND INHERITANCE OF RESISTANCE TO BLACKEYE COWPEA MOSAIC VIRUS. - 95 p.

Source: Dissertation Abstracts International, Volume: 42-04, Section: B, page: 1255.

Thesis (Ph.D.)--Cornell University, 1981.

The biological, physical, immunological and some biochemical properties of single isolates of blackeye cowpea mosaic virus (BlCMV) from Florida and New York were compared with those of single isolates of cowpea aphid-borne mosaic virus (CAMV) from Cyprus, Morocco, Kenya and Nigeria.Subjects--Topical Terms:

1028950
Agriculture, Plant Pathology.

PARTIAL CHARACTERIZATION AND GROUPING OF ISOLATES OF BLACKEYE COWPEA AND COWPEA APHID-BORNE MOSAIC VIRUSES; AND INHERITANCE OF RESISTANCE TO BLACKEYE COWPEA MOSAIC VIRUS.
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008 130610s1981 d
035 $a (UnM)AAI8119429
035 $a AAI8119429
040 $a UnM $c UnM
100 1 $a TAIWO, MONILOLA ARINOLA. $3 1912461
245 1 0 $a PARTIAL CHARACTERIZATION AND GROUPING OF ISOLATES OF BLACKEYE COWPEA AND COWPEA APHID-BORNE MOSAIC VIRUSES; AND INHERITANCE OF RESISTANCE TO BLACKEYE COWPEA MOSAIC VIRUS.
300 $a 95 p.
500 $a Source: Dissertation Abstracts International, Volume: 42-04, Section: B, page: 1255.
502 $a Thesis (Ph.D.)--Cornell University, 1981.
520 $a The biological, physical, immunological and some biochemical properties of single isolates of blackeye cowpea mosaic virus (BlCMV) from Florida and New York were compared with those of single isolates of cowpea aphid-borne mosaic virus (CAMV) from Cyprus, Morocco, Kenya and Nigeria.
520 $a Using antisera prepared to each of the six isolates, serological tests including enzyme-linked immunosorbent assay (ELISA), immune electron microscopy (IEM), and Ouchterlony double diffusion in the presence of sodium dodecyl sulfate (SDS) were used to compare the isolates. Results from these tests showed that the isolates could be divided into two serological groups which were defined as BlCMV and CAMV. The BlCMV group was comprised of both isolates of BlCMV and the Kenya and Nigeria isolates of CAMV. The CAMV group contained the Morocco and Cyprus isolates. In all tests, isolates within a group showed very close, if not identical, serological relationship. Antisera to isolates of the CAMV group did not detectably react to isolates of the BlCMV group. Similarly, antisera to the Florida and Nigeria isolates did not react with members of the CAMV group in SDS-immunodiffusion and ELISA tests. However, antisera from bleedings taken 16 wk or more after injecting rabbits with the New York and Kenya isolates reacted weakly with Morocco and Cyprus isolates in SDS-immunodiffusion tests. In IEM tests, antisera to all isolates in the BlCMV group moderately decorated the isolates in the CAMV group, but no decoration was observed in the reciprocal test.
520 $a Further characterization of these six isolates on the basis of their biological property confirmed the grouping made on the basis of their immunological property. On the basis of their reaction on selected cowpea lines, the six isolates were again classified into the BlCMV or CAMV group. No common source of resistance was found for both CAMV and BlCMV, but cowpea lines possessing resistance to each virus group were identified. These resistant lines were used for differentiating the isolates as BlCMV or CAMV. The BlCMV group consisted of the Florida and New York isolates of BlCMV and the Kenya and Nigeria isolates of CAMV, whereas the CAMV group included the Morocco and Cyprus isolates. In reciprocal cross protection tests, neither the Kenya nor Florida isolates would cross protect against the Morocco isolate, and vice versa. However, the size of the capsid protein, the sedimentation rate of the nucleic acid, and the length of the particles of the six isolates were very similar. The results presented indicate that although CAMV and BlCMV have similar physical properties, they can be grouped according to differences in host range on cowpea lines and their immunological properties. Moreover, two of the isolates previously regarded as CAMV were identical to BlCMV.
520 $a Crosses between the resistant cowpea line TVU 2480 obtained from the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria and the susceptible domestic cultivar Early Ramshorn were used to determine the inheritance of resistance to BlCMV. Evaluation of F(,1), F(,2), and reciprocal backcross populations by symptomatology and ELISA clearly indicated that a single recessive gene controls the high level of resistance. The symbol bcm (blackeye cowpea mosaic) is assigned to this factor. IITA cowpea lines TVU 2740, TVU 3273, TVU 2657, and TVU 2845 seem to possess this same gene.
590 $a School code: 0058.
650 4 $a Agriculture, Plant Pathology. $3 1028950
690 $a 0480
710 2 0 $a Cornell University. $3 530586
773 0 $t Dissertation Abstracts International $g 42-04B.
790 $a 0058
791 $a Ph.D.
792 $a 1981
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=8119429