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Quantities of prostaglandins in whol...

Jaeger, John R.

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Quantities of prostaglandins in whole and extended bovine semen and their potential effect on fertility following insemination.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Quantities of prostaglandins in whole and extended bovine semen and their potential effect on fertility following insemination./
Author:	Jaeger, John R.
Description:	171 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-10, Section: B, page: 5170.
Contained By:	Dissertation Abstracts International66-10B.
Subject:	Biology, Animal Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3194287
ISBN:	9780542377686

Quantities of prostaglandins in whole and extended bovine semen and their potential effect on fertility following insemination.
Jaeger, John R.

Quantities of prostaglandins in whole and extended bovine semen and their potential effect on fertility following insemination. - 171 p.

Source: Dissertation Abstracts International, Volume: 66-10, Section: B, page: 5170.

Thesis (Ph.D.)--Oregon State University, 2005.

Our objectives were to determine (1) the concentration of prostaglandin F2alpha (PGF2alpha) in whole and extended bovine semen, (2) if concentrations of prostaglandins in extended bovine semen are correlated to fertility, and (3) if PGF2alpha administered at the time of artificial insemination would improve conception rate. Concentration of PGF2alpha tended to be only slightly greater for whole compared to extended semen. To elucidate why PGF2alpha levels were comparable, semen was extended at eight dilution rates. Prostaglandin F2alpha in sub-samples, collected during extension, decreased at higher dilution rates and later steps of extension. To quantify PGF2alpha synthesized during extension, quantities of PGF2alpha in semen and the diluent were subtracted from each step. Higher dilution rates reduced the final amount of PGF 2alpha synthesized. Initial PGF2alpha concentration was greater in whole semen compared to seminal plasma; however, when extended at three dilution rates quantity of PGF2alpha synthesized during extension was greater for semen compared to seminal plasma, indicating less disparity than for original samples. Concentrations of prostaglandin E 2 (PGE2) and PGF2alpha in extended semen and the ratio of PGE2 to PGF2alpha were compared to a fertility rating. The ratio of PGE2 to PGF2alpha was not correlated to the fertility rating. Semen was extended to contain either 0, 500, or 5000 pg/ml of exogenous PGF2alpha. Exogenous PGF 2alpha did not affect post-thaw motility or proportion of normal spermatozoa. In vitro fertilization did not differ between treatments. Analysis of cleavage rate per embryo revealed a bull x semen treatment interaction. First service conception rate of cows inseminated with the PGF2alpha -enhanced semen was affected by semen treatment and technician. Beef heifers inseminated with subfertile semen and dairy cows inseminated with normal fertility semen and treated with PGF2alpha after insemination displayed higher conception rates compared to control animals. These data suggest that, although extension reduces the concentration of many seminal components, PGF2alpha synthesis during extension results in concentrations similar to whole semen. Seminal prostaglandin concentrations may be related to fertilizing capability of the semen, and exogenous PGF2alpha administered at the time of insemination may improve conception rate.

ISBN: 9780542377686Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Quantities of prostaglandins in whole and extended bovine semen and their potential effect on fertility following insemination.
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502 $a Thesis (Ph.D.)--Oregon State University, 2005.
520 $a Our objectives were to determine (1) the concentration of prostaglandin F2alpha (PGF2alpha) in whole and extended bovine semen, (2) if concentrations of prostaglandins in extended bovine semen are correlated to fertility, and (3) if PGF2alpha administered at the time of artificial insemination would improve conception rate. Concentration of PGF2alpha tended to be only slightly greater for whole compared to extended semen. To elucidate why PGF2alpha levels were comparable, semen was extended at eight dilution rates. Prostaglandin F2alpha in sub-samples, collected during extension, decreased at higher dilution rates and later steps of extension. To quantify PGF2alpha synthesized during extension, quantities of PGF2alpha in semen and the diluent were subtracted from each step. Higher dilution rates reduced the final amount of PGF 2alpha synthesized. Initial PGF2alpha concentration was greater in whole semen compared to seminal plasma; however, when extended at three dilution rates quantity of PGF2alpha synthesized during extension was greater for semen compared to seminal plasma, indicating less disparity than for original samples. Concentrations of prostaglandin E 2 (PGE2) and PGF2alpha in extended semen and the ratio of PGE2 to PGF2alpha were compared to a fertility rating. The ratio of PGE2 to PGF2alpha was not correlated to the fertility rating. Semen was extended to contain either 0, 500, or 5000 pg/ml of exogenous PGF2alpha. Exogenous PGF 2alpha did not affect post-thaw motility or proportion of normal spermatozoa. In vitro fertilization did not differ between treatments. Analysis of cleavage rate per embryo revealed a bull x semen treatment interaction. First service conception rate of cows inseminated with the PGF2alpha -enhanced semen was affected by semen treatment and technician. Beef heifers inseminated with subfertile semen and dairy cows inseminated with normal fertility semen and treated with PGF2alpha after insemination displayed higher conception rates compared to control animals. These data suggest that, although extension reduces the concentration of many seminal components, PGF2alpha synthesis during extension results in concentrations similar to whole semen. Seminal prostaglandin concentrations may be related to fertilizing capability of the semen, and exogenous PGF2alpha administered at the time of insemination may improve conception rate.
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