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Application and transgenic productio...

Settavongsin, Rachada.

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Application and transgenic production of the biosurfactant cyclodextrin for enhanced polyaromatic hydrocarbon phytoremediation.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Application and transgenic production of the biosurfactant cyclodextrin for enhanced polyaromatic hydrocarbon phytoremediation./
Author:	Settavongsin, Rachada.
Description:	175 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-09, Section: B, page: 4564.
Contained By:	Dissertation Abstracts International66-09B.
Subject:	Agriculture, Soil Science. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3189738
ISBN:	9780542328091

Application and transgenic production of the biosurfactant cyclodextrin for enhanced polyaromatic hydrocarbon phytoremediation.
Settavongsin, Rachada.

Application and transgenic production of the biosurfactant cyclodextrin for enhanced polyaromatic hydrocarbon phytoremediation. - 175 p.

Source: Dissertation Abstracts International, Volume: 66-09, Section: B, page: 4564.

Thesis (Ph.D.)--Michigan State University, 2005.

Phytoremediation is the use of plants to cleanup organic and inorganic contaminants and is considered a cost effective, aesthetically pleasing, and more environmentally friendly alternative to engineering-based remediation. Plant roots and their associated microbes are considered the major mechanism for phytoremediation of some persistent organic pollutants such as polycyclic aromatic hydrocarbons (PAHs). PAHs are carcinogenic and mutagenic compounds that are stably sorbed to soil organic matter, which limits aqueous solubility and consequently biodegradation effectiveness. Biosurfactants are biologically synthesized detergent-like compounds that have been demonstrated to improve bioremediation effectiveness for some pollutants. Cyclodextrins (CDs) are sugar-based biosurfactants produced from starch by the bacterial enzyme cyclodextrin glycosyltransferase (CGTase). CDs have been shown to enhance solubility of various organic and metal contaminants from polluted soils including enhanced biodegradation of the PAH compound phenanthrene.

ISBN: 9780542328091Subjects--Topical Terms:

1017824
Agriculture, Soil Science.

Application and transgenic production of the biosurfactant cyclodextrin for enhanced polyaromatic hydrocarbon phytoremediation.
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100 1 $a Settavongsin, Rachada. $3 1912026
245 1 0 $a Application and transgenic production of the biosurfactant cyclodextrin for enhanced polyaromatic hydrocarbon phytoremediation.
300 $a 175 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-09, Section: B, page: 4564.
500 $a Adviser: Clayton L. Rugh.
502 $a Thesis (Ph.D.)--Michigan State University, 2005.
520 $a Phytoremediation is the use of plants to cleanup organic and inorganic contaminants and is considered a cost effective, aesthetically pleasing, and more environmentally friendly alternative to engineering-based remediation. Plant roots and their associated microbes are considered the major mechanism for phytoremediation of some persistent organic pollutants such as polycyclic aromatic hydrocarbons (PAHs). PAHs are carcinogenic and mutagenic compounds that are stably sorbed to soil organic matter, which limits aqueous solubility and consequently biodegradation effectiveness. Biosurfactants are biologically synthesized detergent-like compounds that have been demonstrated to improve bioremediation effectiveness for some pollutants. Cyclodextrins (CDs) are sugar-based biosurfactants produced from starch by the bacterial enzyme cyclodextrin glycosyltransferase (CGTase). CDs have been shown to enhance solubility of various organic and metal contaminants from polluted soils including enhanced biodegradation of the PAH compound phenanthrene.
520 $a We hypothesized that the presence of biosurfactants (CDs) in the rhizosphere will improve bioremediation effectiveness by enhanced solubilization of otherwise persistent organic pollutants and proliferation of PAH biodegrading bacteria. Beta-cyclodextrin (betaCD) amendments were tested in a 4-month greenhouse study of four phytoremediation plant species grown in PAH-impacted, industrial waste soils. Soil PAH levels and bacterial community response were evaluated in betaCD treated and untreated control soils. betaCD amendments were demonstrated to enhance both PAH bioremediation effectiveness and stimulate total bacterial and biodegrader cell densities in most plant + biosurfactant treatment combinations. PAH levels were generally observed to be more effectively reduced in betaCD treated rhizospheres relative to unamended control soils.
520 $a As a strategy to biologically generate betaCD in contaminated soils for in situ biosurfactant-enhanced phytoremediation, we genetically engineered transgenic plants transformed with the bacterial cyclodextrin glycosyltransferase (cgt) gene. The cgt gene of the betaCD-producing bacterium Paenibacillus illinoisensis (PI) was cloned and expressed in tobacco (Nicotiana tabacum). Transgenic PI- cgt plants produced and secreted an extracellular enzyme, which was shown to degrade starch in culture medium. Preliminary assays of cgt-tobacco in PAH-spiked soils were not conclusive for clear indication of biodegradation. It is anticipated that cgt-tobacco "phyto"-betaCD will enhance solubization and subsequent biodegradation of otherwise persistent organic pollutants.
590 $a School code: 0128.
650 4 $a Agriculture, Soil Science. $3 1017824
650 4 $a Engineering, Environmental. $3 783782
690 $a 0481
690 $a 0775
710 2 0 $a Michigan State University. $3 676168
773 0 $t Dissertation Abstracts International $g 66-09B.
790 1 0 $a Rugh, Clayton L., $e advisor
790 $a 0128
791 $a Ph.D.
792 $a 2005
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