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Integrated approaches to virulence g...

Yang, Shihui.

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Integrated approaches to virulence gene discovery of Erwinia chrysanthemi 3937.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Integrated approaches to virulence gene discovery of Erwinia chrysanthemi 3937./
Author:	Yang, Shihui.
Description:	109 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-03, Section: B, page: 1293.
Contained By:	Dissertation Abstracts International66-03B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3168257
ISBN:	9780542044427

Integrated approaches to virulence gene discovery of Erwinia chrysanthemi 3937.
Yang, Shihui.

Integrated approaches to virulence gene discovery of Erwinia chrysanthemi 3937. - 109 p.

Source: Dissertation Abstracts International, Volume: 66-03, Section: B, page: 1293.

Thesis (Ph.D.)--University of California, Riverside, 2005.

Erwinia chrysanthemi is an opportunistic plant pathogen that causes economically important soft-rot, wilt, and blight diseases on a wide range of plant species. This organism uses complex regulation strategies for virulence gene expression. Beyond identification of virulence genes in E. chrysanthemi 3937, a more detailed understanding of host-regulated genes in this bacterium and the pathogen-host interaction mechanism are needed. In this dissertation, using a GFP (green fluorescent protein) based IVET (in vivo expression technology) leaf array, sixty-one plant-upregulated genes of E. chrysanthemi 3937 were identified and their probable functions categorized. Nine independent mutants were constructed, and mutants of purM::Tn5, hrpB::Tn5, hrcJ::Tn5, and hrpD::Tn5 exhibited reduced abilities to produce local and systemic maceration of the plant host. Mutants of rhiT::Tn5 and eutR::Tn5 showed decreased ability to cause systemic invasion on African violet.

ISBN: 9780542044427Subjects--Topical Terms:

1017734
Biology, Microbiology.

Integrated approaches to virulence gene discovery of Erwinia chrysanthemi 3937.
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100 1 $a Yang, Shihui. $3 1911980
245 1 0 $a Integrated approaches to virulence gene discovery of Erwinia chrysanthemi 3937.
300 $a 109 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-03, Section: B, page: 1293.
500 $a Chairs: Donald A. Cooksey; Ching-Hong Yang.
502 $a Thesis (Ph.D.)--University of California, Riverside, 2005.
520 $a Erwinia chrysanthemi is an opportunistic plant pathogen that causes economically important soft-rot, wilt, and blight diseases on a wide range of plant species. This organism uses complex regulation strategies for virulence gene expression. Beyond identification of virulence genes in E. chrysanthemi 3937, a more detailed understanding of host-regulated genes in this bacterium and the pathogen-host interaction mechanism are needed. In this dissertation, using a GFP (green fluorescent protein) based IVET (in vivo expression technology) leaf array, sixty-one plant-upregulated genes of E. chrysanthemi 3937 were identified and their probable functions categorized. Nine independent mutants were constructed, and mutants of purM::Tn5, hrpB::Tn5, hrcJ::Tn5, and hrpD::Tn5 exhibited reduced abilities to produce local and systemic maceration of the plant host. Mutants of rhiT::Tn5 and eutR::Tn5 showed decreased ability to cause systemic invasion on African violet.
520 $a An insertional knockout (iaaM::KAN) of one plant-upregulated gene, iaaM, which participates in phytohormone indoleacetic-3-acid (IAA) production through the indole-3-acetamide pathway, was constructed and further characterized. The iaaM mutant produced less IAA in minimal medium supplemented with L-tryptophan and formed much less pellicle-biofilm. In contrast, pectate lyase production and spreading motility were both enhanced in the iaaM mutant, as was local maceration in Saintpaulia ionantha (African violet) cv. Gaugil. Populations of the iaaM mutant did not reach levels as high as the wild-type strain in infiltrated leaves. An IAA over-producing strain also had enhanced pectate lyase production, but its ability to cause local maceration was greatly reduced, as was observed with the addition of IAA to the inoculum of the wild-type strain. Optimal IAA production is important for the in planta fitness of E. chrysanthemi 3937. Disruption of the IAA biosynthetic pathway affects pleiotropic phenotypes as well as pathogenicity of E. chrysanthemi 3937.
590 $a School code: 0032.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Agriculture, Plant Pathology. $3 1028950
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690 $a 0480
710 2 0 $a University of California, Riverside. $3 791122
773 0 $t Dissertation Abstracts International $g 66-03B.
790 1 0 $a Cooksey, Donald A., $e advisor
790 1 0 $a Yang, Ching-Hong, $e advisor
790 $a 0032
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3168257