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Influence of scaffold properties on ...

Huang, Yan.

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Influence of scaffold properties on cellular colonization for tissue engineering.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Influence of scaffold properties on cellular colonization for tissue engineering./
作者:	Huang, Yan.
面頁冊數:	176 p.
附註:	Source: Dissertation Abstracts International, Volume: 66-06, Section: B, page: 3271.
Contained By:	Dissertation Abstracts International66-06B.
標題:	Engineering, Chemical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3178302
ISBN:	9780542179181

Influence of scaffold properties on cellular colonization for tissue engineering.
Huang, Yan.

Influence of scaffold properties on cellular colonization for tissue engineering. - 176 p.

Source: Dissertation Abstracts International, Volume: 66-06, Section: B, page: 3271.

Thesis (Ph.D.)--Oklahoma State University, 2005.

Scope and method of study. Scaffolds properties play crucial roles in regulating the cell-matrix interaction. To understand how the scaffolds architecture (i.e. pore structures), the presence of cell-binding domain and stiffness affect cell colonization within the scaffolds, 2D and 3D chitosan and poly (lactide-co-glycolide) (PLGA)-chitosan scaffolds without cell-binding domain were synthesized through controlled rate freezing and lyophilization. Cytoskeletal organization, morphology, and proliferation of Human umbilical vein endothelial cells (HUVECs) and mouse embryonic fibroblasts (MEFs) seeded on 2D, 3D chitosan, PLGA-chitosan scaffolds were characterized. Next, gelatin which contains cell-binding domain was blended with chitosan to investigate cell adhesion characteristics through receptor-mediated binding under static and shear conditions. Murine embryonic stem (mES) cells were used as model to study the differentiation process under the influence of substratum and medium components (i.e. cytokines).

ISBN: 9780542179181Subjects--Topical Terms:

1018531
Engineering, Chemical.

Influence of scaffold properties on cellular colonization for tissue engineering.
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245 1 0 $a Influence of scaffold properties on cellular colonization for tissue engineering.
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500 $a Source: Dissertation Abstracts International, Volume: 66-06, Section: B, page: 3271.
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502 $a Thesis (Ph.D.)--Oklahoma State University, 2005.
520 $a Scope and method of study. Scaffolds properties play crucial roles in regulating the cell-matrix interaction. To understand how the scaffolds architecture (i.e. pore structures), the presence of cell-binding domain and stiffness affect cell colonization within the scaffolds, 2D and 3D chitosan and poly (lactide-co-glycolide) (PLGA)-chitosan scaffolds without cell-binding domain were synthesized through controlled rate freezing and lyophilization. Cytoskeletal organization, morphology, and proliferation of Human umbilical vein endothelial cells (HUVECs) and mouse embryonic fibroblasts (MEFs) seeded on 2D, 3D chitosan, PLGA-chitosan scaffolds were characterized. Next, gelatin which contains cell-binding domain was blended with chitosan to investigate cell adhesion characteristics through receptor-mediated binding under static and shear conditions. Murine embryonic stem (mES) cells were used as model to study the differentiation process under the influence of substratum and medium components (i.e. cytokines).
520 $a Findings and conclusions. Both HUVECs and MEFs showed circular morphology with reduced spreading area on 2D scaffolds while increased spreading and connected cell organization within 3D chitosan matrices. 2D and 3D emulsified chitosan and PLGA-chitosan scaffolds did not support spreading of HUVECs and MEFs. In gelatin-chitosan membranes, effect of gelatin was dominant; actin and focal adhesion kinase (FAK) distribution were comparable to gelatin in static culture. However, at higher shear stresses, presence of chitosan inhibited shear-induced increase in cell spreading and weakened cell adhesive strength. More composition of gelatin in the 3D chitosan scaffolds inhibited cell spreading within the porous structures. Similarly, the presence of chitosan in the membranes did not support mESachment. These results support the hypothesis that the presence of cell-binding domain in the materials assumes the most important roles in regulating cell adhesion on 2D matrix whereas microarchitecture and stiffness are more important in 3D scaffolds on influencing cellular colonization.
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