東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

High-performance biocatalysts for en...

Cho, Catherine Mee-Hie.

FindBook

Google Book

Amazon

博客來

High-performance biocatalysts for enhanced biodegradation of organophosphorus pesticides: Directed evolution and rational design.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	High-performance biocatalysts for enhanced biodegradation of organophosphorus pesticides: Directed evolution and rational design./
作者:	Cho, Catherine Mee-Hie.
面頁冊數:	94 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3664.
Contained By:	Dissertation Abstracts International64-08B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3100614
ISBN:	9780496482054

High-performance biocatalysts for enhanced biodegradation of organophosphorus pesticides: Directed evolution and rational design.
Cho, Catherine Mee-Hie.

High-performance biocatalysts for enhanced biodegradation of organophosphorus pesticides: Directed evolution and rational design. - 94 p.

Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3664.

Thesis (Ph.D.)--University of California, Riverside, 2003.

The use of pesticides is necessary to increase food production and protect the public from diseases such as malaria. Organophosphates (OP), a class of highly toxic neurotoxins, account for about half of all pesticides used in the U.S. Due to the growing environmental concerns associated with these pesticides in food products and water supplies, there is a great need to develop a proper remediation method to deal with their wide spread contamination. Organophosphorus hydrolase (OPH) isolated from naturally occurring bacteria has been used for the degradation of a variety of organophosphates. Although this enzyme has a broad substrate specificity, the hydrolysis rate varies from diffusion-limited for paraoxon to poorly degraded for pesticides such as methyl parathion and chlorpyrifos and chemical warfare agents such as sarin.

ISBN: 9780496482054Subjects--Topical Terms:

1017719
Biology, Molecular.

High-performance biocatalysts for enhanced biodegradation of organophosphorus pesticides: Directed evolution and rational design.
LDR:03087nmm 2200325 4500 001 1820922
005 20061110072958.5
008 130610s2003 eng d
020 $a 9780496482054
035 $a (UnM)AAI3100614
035 $a AAI3100614
040 $a UnM $c UnM
100 1 $a Cho, Catherine Mee-Hie. $3 1910121
245 1 0 $a High-performance biocatalysts for enhanced biodegradation of organophosphorus pesticides: Directed evolution and rational design.
300 $a 94 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3664.
500 $a Chairs: Wilfred Chen; Ashok Mulchandani.
502 $a Thesis (Ph.D.)--University of California, Riverside, 2003.
520 $a The use of pesticides is necessary to increase food production and protect the public from diseases such as malaria. Organophosphates (OP), a class of highly toxic neurotoxins, account for about half of all pesticides used in the U.S. Due to the growing environmental concerns associated with these pesticides in food products and water supplies, there is a great need to develop a proper remediation method to deal with their wide spread contamination. Organophosphorus hydrolase (OPH) isolated from naturally occurring bacteria has been used for the degradation of a variety of organophosphates. Although this enzyme has a broad substrate specificity, the hydrolysis rate varies from diffusion-limited for paraoxon to poorly degraded for pesticides such as methyl parathion and chlorpyrifos and chemical warfare agents such as sarin.
520 $a Directed evolution was employed to improve the substrate specificity of OPH. Improved variants against methyl parathion were generated after only two rounds of DNA shuffling and screening. The best variant 22A11 hydrolyzed methyl parathion 30 times faster than the wild-type and also degraded paraoxon, parathion, and coumaphos 2--10 times faster. Several distal mutations were identified to be responsible for this global increase in hydrolysis and their importance in determining substrate specificity was investigated by site-directed mutagenesis and saturation mutagenesis. One particular mutation, K185R, was shown to enhance the overall hydrolysis rate when present in conjunction with other mutations.
520 $a Similar efforts to create improved variants for the degradation of chlorpyrifos were also performed. After two generations of DNA shuffling and screening, variants with &sim;745-fold increased in kcat/K m value were obtained. The best variant B3561 also showed improved hydrolysis rates for paraoxon, methyl parathion, parathion, and coumaphos. The results suggest that the variant B3561 is one of the most efficient enzymes available to attack a wide spectrum of nerve agents.
590 $a School code: 0032.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Engineering, Chemical. $3 1018531
650 4 $a Health Sciences, Toxicology. $3 1017752
690 $a 0307
690 $a 0542
690 $a 0383
710 2 0 $a University of California, Riverside. $3 791122
773 0 $t Dissertation Abstracts International $g 64-08B.
790 1 0 $a Chen, Wilfred, $e advisor
790 1 0 $a Mulchandani, Ashok, $e advisor
790 $a 0032
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3100614