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The effect of biopolymer properties ...

Abu-Lail, Nehal Ibrahim.

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The effect of biopolymer properties on bacterial adhesion: An atomic force microscopy (AFM) study.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The effect of biopolymer properties on bacterial adhesion: An atomic force microscopy (AFM) study./
Author:	Abu-Lail, Nehal Ibrahim.
Description:	337 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3391.
Contained By:	Dissertation Abstracts International64-07B.
Subject:	Engineering, Chemical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3097858
ISBN:	9780496454716

The effect of biopolymer properties on bacterial adhesion: An atomic force microscopy (AFM) study.
Abu-Lail, Nehal Ibrahim.

The effect of biopolymer properties on bacterial adhesion: An atomic force microscopy (AFM) study. - 337 p.

Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3391.

Thesis (Ph.D.)--Worcester Polytechnic Institute, 2003.

The effect of bacterial surface biopolymers on bacterial adhesion to surfaces was studied through experiments and modeling. Atomic Force Microscopy (AFM) provided the tool to measure the interaction forces between different bacterial cells and silicon nitride tips under different chemical conditions at a nanoscopic level. Two bacterial strains were considered: Pseudomonas putida KT2442 and Escherichia coli K-12 JM109.

ISBN: 9780496454716Subjects--Topical Terms:

1018531
Engineering, Chemical.

The effect of biopolymer properties on bacterial adhesion: An atomic force microscopy (AFM) study.
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100 1 $a Abu-Lail, Nehal Ibrahim. $3 1910116
245 1 4 $a The effect of biopolymer properties on bacterial adhesion: An atomic force microscopy (AFM) study.
300 $a 337 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3391.
500 $a Adviser: Terri A. Camesano.
502 $a Thesis (Ph.D.)--Worcester Polytechnic Institute, 2003.
520 $a The effect of bacterial surface biopolymers on bacterial adhesion to surfaces was studied through experiments and modeling. Atomic Force Microscopy (AFM) provided the tool to measure the interaction forces between different bacterial cells and silicon nitride tips under different chemical conditions at a nanoscopic level. Two bacterial strains were considered: Pseudomonas putida KT2442 and Escherichia coli K-12 JM109.
520 $a To address the first issue, formamide, water, and methanol were used to investigate the effect of polarity on surface characteristics of biopolymers on the bacterial surface while a range of salt concentrations between that of water to 1 M KCl were used to study the effect of ionic strength. A dramatic increase in the adhesion magnitude was observed when the salt concentration increased above 0.1 M KCl. This transition in adhesion with ionic strength from a low to high value induced a transition in the elasticity of the bacterial surface biopolymers. The biopolymer brush layer did change from rigid to soft with increasing ionic strength. The elasticity was quantified mainly by the use of the freely jointed chain (FJC) model.
520 $a Analyzing the adhesion forces for P. putida KT2442 showed that the bacterial surface is heterogeneous. The heterogeneity was evident on the same cell surface and between different cells from the same population.
520 $a Approximately 80% of the surface LPS of E. coli K-12 JM109 were removed by treating the cells with 100 mM ethylenediaminetetraacetic acid (EDTA). The effect of LPS removal on the adhesion of the cells to the silicon nitride tip was studied in water and phosphate buffered silane (PBS). The adhesion results from the AFM experiments were compared to batch retention experiments with glass as the substratum and column attachment experiments with columns packed with quartz sand. LPS controlled bacterial adhesion to the different surfaces in the study at three scales: batch, continuous, and nano-scale.
520 $a Finally, the nature of interactions between E. coli JM109 and a model surface (silicon nitride tip) were investigated in solvents of varying polarity (formamide, water, and methanol). The Young's modulus of elasticity for the bacterial surface was estimated by fitting of the Hertzian model to the force-indentation curves. Young's modulus values increased as the solvent polarity decreased, indicating a stiffer bacterial surface in lower polarity solvents. The average adhesion force in each solvent was negatively correlated with the dielectric constant of the solvent, suggesting hydrophilic biopolymers. Specific and non-specific interaction forces between the AFM tip and the biopolymers were further characterized by applying a Poisson statistical analysis to the discrete adhesion data. (Abstract shortened by UMI.)
590 $a School code: 0774.
650 4 $a Engineering, Chemical. $3 1018531
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0542
690 $a 0410
710 2 0 $a Worcester Polytechnic Institute. $3 1025154
773 0 $t Dissertation Abstracts International $g 64-07B.
790 1 0 $a Camesano, Terri A., $e advisor
790 $a 0774
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3097858