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Molecular analysis of two photosynth...

Lu, Taihe.

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Molecular analysis of two photosynthetic genes in a parasitic plant, Cuscuta.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Molecular analysis of two photosynthetic genes in a parasitic plant, Cuscuta./
Author:	Lu, Taihe.
Description:	110 p.
Notes:	Source: Dissertation Abstracts International, Volume: 54-05, Section: B, page: 2363.
Contained By:	Dissertation Abstracts International54-05B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9328007

Molecular analysis of two photosynthetic genes in a parasitic plant, Cuscuta.
Lu, Taihe.

Molecular analysis of two photosynthetic genes in a parasitic plant, Cuscuta. - 110 p.

Source: Dissertation Abstracts International, Volume: 54-05, Section: B, page: 2363.

Thesis (Ph.D.)--The University of Wisconsin - Milwaukee, 1993.

5 and Cuscuta is a facultatively photosynthetic parasite. The DNA sequence of Cuscuta compacta and Cuscuta gronovii psbA andCuscuta compacta psaA were determined. The psbA sequence identities of two dodder genes is only about 88% compared to those of tobacco. A phylogenetic tree based on the psbA DNA sequences shows that the relationships of 11 higher plants matches the traditional classification very well. The long branch of the psbA phylogenetic tree for the two dodder species shows rapid evolution. The amino acid sequences of the D1 and A1 proteins of dodder show higher identities in the regions which are related to the biological functions of those proteins. Two DNA fragments containing the psbA gene were isolated by screening a genomic library, ligated into a plasmid vector, and sequenced. The result shows that the start codon of dodder psbA is GTG, not ATG. The DNA sequence in the promoter region of psbA is totally different from that of other plants except the Subjects--Topical Terms:

1017719
Biology, Molecular.

Molecular analysis of two photosynthetic genes in a parasitic plant, Cuscuta.
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035 $a (UnM)AAI9328007
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040 $a UnM $c UnM
100 1 $a Lu, Taihe. $3 1908344
245 1 0 $a Molecular analysis of two photosynthetic genes in a parasitic plant, Cuscuta.
300 $a 110 p.
500 $a Source: Dissertation Abstracts International, Volume: 54-05, Section: B, page: 2363.
500 $a Supervisor: Charles Wimpee.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Milwaukee, 1993.
520 $a Cuscuta is a facultatively photosynthetic parasite. The DNA sequence of Cuscuta compacta and Cuscuta gronovii psbA andCuscuta compacta psaA were determined. The psbA sequence identities of two dodder genes is only about 88% compared to those of tobacco. A phylogenetic tree based on the psbA DNA sequences shows that the relationships of 11 higher plants matches the traditional classification very well. The long branch of the psbA phylogenetic tree for the two dodder species shows rapid evolution. The amino acid sequences of the D1 and A1 proteins of dodder show higher identities in the regions which are related to the biological functions of those proteins. Two DNA fragments containing the psbA gene were isolated by screening a genomic library, ligated into a plasmid vector, and sequenced. The result shows that the start codon of dodder psbA is GTG, not ATG. The DNA sequence in the promoter region of psbA is totally different from that of other plants except the $- $3 5 and $- $1 0 consensus elements. The region between psbA and trnH is only 93 bp, lacking any stem-loop structure. The northern blotting result shows that mRNA of psbA is present in both dark and light grown seedlings of dodder, while it only occurs in light grown seedlings of morning glory.
590 $a School code: 0263.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Botany. $3 1017825
690 $a 0307
690 $a 0309
710 2 0 $a The University of Wisconsin - Milwaukee. $3 1019345
773 0 $t Dissertation Abstracts International $g 54-05B.
790 1 0 $a Wimpee, Charles, $e advisor
790 $a 0263
791 $a Ph.D.
792 $a 1993
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9328007