東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Retroviral RNA nuclear export elemen...

Swanson, Chad Michael.

Linked to FindBook

Google Book

Amazon

博客來

Retroviral RNA nuclear export elements regulate protein function and viral assembly.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Retroviral RNA nuclear export elements regulate protein function and viral assembly./
Author:	Swanson, Chad Michael.
Description:	181 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5539.
Contained By:	Dissertation Abstracts International65-11B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3152107
ISBN:	0496125079

Retroviral RNA nuclear export elements regulate protein function and viral assembly.
Swanson, Chad Michael.

Retroviral RNA nuclear export elements regulate protein function and viral assembly. - 181 p.

Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5539.

Thesis (Ph.D.)--University of Pennsylvania, 2004.

Recently, it has been recognized that mRNA-associated events occurring in the nucleus can regulate the fate of the mRNA in the cytosol. We address the hypothesis that the nuclear history of HIV gag-pol RNA can alter the activity of the encoded protein. In human cells, HIV unspliced RNA normally uses the Crm1 nuclear export pathway in an RRE/Rev-dependent manner. Routing gag containing RNA nuclear export to another pathway disrupts Gag assembly and particle production for several independent constructs. However, in the context of four tandem copies of the M-PMV CTE (4 x CTE), gag-pol RNA using the NXF1 nuclear export pathway is translated into Gag protein that can productively assemble. Murine cells are notable for their inability to support normal assembly of HIV particles and we show that altering the RNA nuclear export element used by HIV gag-pol mRNA from the RRE to the 4 x CTE restores both the trafficking of Gag to cellular membranes and efficient particle production in these cells. These results suggest that two phases of the HIV life cycle, RNA export and capsid assembly, that have hitherto been regarded as distinct are, in fact, linked. Thus, protein function and fate may depend upon the full and precise history of its encoding mRNA.

ISBN: 0496125079Subjects--Topical Terms:

1017734
Biology, Microbiology.

Retroviral RNA nuclear export elements regulate protein function and viral assembly.
LDR:02143nmm 2200265 4500 001 1818941
005 20061003091751.5
008 130610s2004 eng d
020 $a 0496125079
035 $a (UnM)AAI3152107
035 $a AAI3152107
040 $a UnM $c UnM
100 1 $a Swanson, Chad Michael. $3 1908244
245 1 0 $a Retroviral RNA nuclear export elements regulate protein function and viral assembly.
300 $a 181 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5539.
500 $a Supervisor: Michael H. Malim.
502 $a Thesis (Ph.D.)--University of Pennsylvania, 2004.
520 $a Recently, it has been recognized that mRNA-associated events occurring in the nucleus can regulate the fate of the mRNA in the cytosol. We address the hypothesis that the nuclear history of HIV gag-pol RNA can alter the activity of the encoded protein. In human cells, HIV unspliced RNA normally uses the Crm1 nuclear export pathway in an RRE/Rev-dependent manner. Routing gag containing RNA nuclear export to another pathway disrupts Gag assembly and particle production for several independent constructs. However, in the context of four tandem copies of the M-PMV CTE (4 x CTE), gag-pol RNA using the NXF1 nuclear export pathway is translated into Gag protein that can productively assemble. Murine cells are notable for their inability to support normal assembly of HIV particles and we show that altering the RNA nuclear export element used by HIV gag-pol mRNA from the RRE to the 4 x CTE restores both the trafficking of Gag to cellular membranes and efficient particle production in these cells. These results suggest that two phases of the HIV life cycle, RNA export and capsid assembly, that have hitherto been regarded as distinct are, in fact, linked. Thus, protein function and fate may depend upon the full and precise history of its encoding mRNA.
590 $a School code: 0175.
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0410
710 2 0 $a University of Pennsylvania. $3 1017401
773 0 $t Dissertation Abstracts International $g 65-11B.
790 1 0 $a Malim, Michael H., $e advisor
790 $a 0175
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3152107