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Analyses of genes controlling circad...

Strayer, Carl Alan.

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Analyses of genes controlling circadian rhythms in Arabidopsis thaliana.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Analyses of genes controlling circadian rhythms in Arabidopsis thaliana./
Author:	Strayer, Carl Alan.
Description:	226 p.
Notes:	Source: Dissertation Abstracts International, Volume: 62-02, Section: B, page: 0708.
Contained By:	Dissertation Abstracts International62-02B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3003851
ISBN:	049312716X

Analyses of genes controlling circadian rhythms in Arabidopsis thaliana.
Strayer, Carl Alan.

Analyses of genes controlling circadian rhythms in Arabidopsis thaliana. - 226 p.

Source: Dissertation Abstracts International, Volume: 62-02, Section: B, page: 0708.

Thesis (Ph.D.)--University of Virginia, 2001.

One mechanism that plants employ to anticipate and better adapt to changes in their environment is the endogenous circadian clock, which temporally regulates most physiological processes. In order to better understand the molecular basis of circadian timing in plants, genetic investigations were undertaken in the model species Arabidopsis thaliana. First, a positional cloning strategy was used to isolate the TOC1 gene, previously identified in a genetic screen for circadian rhythm mutants. toc1-1 mutation shortens the period of multiple circadian outputs, and these and other phenotypes suggest it encodes a circadian pacemaker component. Characterization of the cloned TOC1 gene and its products provided further evidence consistent with this hypothesis. TOC1 is a nuclear protein that contains a novel type of response regulator receiver domain and two motifs that suggest a role in transcriptional regulation, including a basic motif conserved within the CONSTANS family of transcription factors. TOC1 is circadianly regulated and participates in a feedback loop to control its own expression, hallmarks of pacemaker genes from other organisms. Other work showed that toc1 mutation shortens period in constant darkness. This finding further supports the notion that TOC1 functions within the pacemaker itself, rather than modulating circadian period through function in light signaling to the clock.

ISBN: 049312716XSubjects--Topical Terms:

1017719
Biology, Molecular.

Analyses of genes controlling circadian rhythms in Arabidopsis thaliana.
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100 1 $a Strayer, Carl Alan. $3 1907199
245 1 0 $a Analyses of genes controlling circadian rhythms in Arabidopsis thaliana.
300 $a 226 p.
500 $a Source: Dissertation Abstracts International, Volume: 62-02, Section: B, page: 0708.
500 $a Adviser: Steve A. Kay.
502 $a Thesis (Ph.D.)--University of Virginia, 2001.
520 $a One mechanism that plants employ to anticipate and better adapt to changes in their environment is the endogenous circadian clock, which temporally regulates most physiological processes. In order to better understand the molecular basis of circadian timing in plants, genetic investigations were undertaken in the model species Arabidopsis thaliana. First, a positional cloning strategy was used to isolate the TOC1 gene, previously identified in a genetic screen for circadian rhythm mutants. toc1-1 mutation shortens the period of multiple circadian outputs, and these and other phenotypes suggest it encodes a circadian pacemaker component. Characterization of the cloned TOC1 gene and its products provided further evidence consistent with this hypothesis. TOC1 is a nuclear protein that contains a novel type of response regulator receiver domain and two motifs that suggest a role in transcriptional regulation, including a basic motif conserved within the CONSTANS family of transcription factors. TOC1 is circadianly regulated and participates in a feedback loop to control its own expression, hallmarks of pacemaker genes from other organisms. Other work showed that toc1 mutation shortens period in constant darkness. This finding further supports the notion that TOC1 functions within the pacemaker itself, rather than modulating circadian period through function in light signaling to the clock.
520 $a In other experiments, the circadian function of CONSTANS and two closely related genes, COL1 and COL2, were investigated. Previous studies demonstrated that mis-expression of CONSTANS disrupts photoperiodic flowering, a process regulated by the circadian clock. In this work, mis-expression of CONSTANS was shown to have no effect on circadian rhythms. In contrast, mis-expression of COL1 or COL2 did not disrupt photoperiodic flowering, but COL1 overexpression did significantly shorten the period of some circadian rhythms. Evidence suggests the period-shortening effect is due to changes in light input pathways leading to the pacemaker controlling these rhythms.
520 $a Finally, the organization of circadian systems in Arabidopsis was investigated. While over-expression of either COL1 or PHYB (which encodes a circadian photoreceptor) shortens CAB2 gene expression rhythms, CCR2 gene expression rhythms are unaffected in either background. A differential effect on these two rhythms strongly suggests differently comprised circadian systems control them.
590 $a School code: 0246.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Plant Physiology. $3 1017865
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690 $a 0369
690 $a 0817
710 2 0 $a University of Virginia. $3 645578
773 0 $t Dissertation Abstracts International $g 62-02B.
790 1 0 $a Kay, Steve A., $e advisor
790 $a 0246
791 $a Ph.D.
792 $a 2001
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3003851