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Studies on the role ofperiod,timeles...

Lee, Choogon.

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Studies on the role ofperiod,timeless,dclock andcycle in the transcriptional feedback loop and photic entrainment of the Drosophila circadian clock.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Studies on the role ofperiod,timeless,dclock andcycle in the transcriptional feedback loop and photic entrainment of the Drosophila circadian clock./
作者:	Lee, Choogon.
面頁冊數:	162 p.
附註:	Source: Dissertation Abstracts International, Volume: 60-01, Section: B, page: 0170.
Contained By:	Dissertation Abstracts International60-01B.
標題:	Chemistry, Biochemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9919737
ISBN:	0599185910

Studies on the role ofperiod,timeless,dclock andcycle in the transcriptional feedback loop and photic entrainment of the Drosophila circadian clock.
Lee, Choogon.

Studies on the role ofperiod,timeless,dclock andcycle in the transcriptional feedback loop and photic entrainment of the Drosophila circadian clock. - 162 p.

Source: Dissertation Abstracts International, Volume: 60-01, Section: B, page: 0170.

Thesis (Ph.D.)--Rutgers The State University of New Jersey - New Brunswick and University of Medicine and Dentistry of New Jersey, 1999.

Circadian (&cong;24) rhythms are found in a wide variety of organisms, including bacteria, plants and humans. These rhythms are governed by intracellular clocks that can be synchronized (entrained) by daily changes in light and temperature.

ISBN: 0599185910Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Studies on the role ofperiod,timeless,dclock andcycle in the transcriptional feedback loop and photic entrainment of the Drosophila circadian clock.
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100 1 $a Lee, Choogon. $3 1907170
245 1 0 $a Studies on the role ofperiod,timeless,dclock andcycle in the transcriptional feedback loop and photic entrainment of the Drosophila circadian clock.
300 $a 162 p.
500 $a Source: Dissertation Abstracts International, Volume: 60-01, Section: B, page: 0170.
500 $a Director: Isaac Edery.
502 $a Thesis (Ph.D.)--Rutgers The State University of New Jersey - New Brunswick and University of Medicine and Dentistry of New Jersey, 1999.
520 $a Circadian (&cong;24) rhythms are found in a wide variety of organisms, including bacteria, plants and humans. These rhythms are governed by intracellular clocks that can be synchronized (entrained) by daily changes in light and temperature.
520 $a Molecular and genetic studies have revealed several clock genes in Drosophila which are required for the function of the circadian pacemaker. The protein and mRNA products of per and tim undergo daily cycles in abundance. Both per and tim transcription are positively regulated by a complex of two transcription factors, dCLOCK and CYCLE, which interact with cis-acting elements (E-box) present in both per and tim promoter regions. This positive regulation is suppressed by nuclear PER/TIM proteins through negative feedback regulation which appears to be a conserved feature of circadian time keeping mechanisms. At the time my thesis started only per and tim had been isolated in Drosophila, and it was not known how light regulates the time keeping mechanism.
520 $a My thesis concerns the molecular mechanism underlying circadian rhythms and can be divided into two main topics: (1)�photic entrainment pathway and (2)�molecular organization of the clock machinery.
520 $a To study the photic entrainment mechanism, I adopted a perturbation strategy, in which the circadian pacemaker was perturbed by light pulses, and the temporal regulation of per was studied. In this study, PER and TIM were shown to interact in vivo, which likely explains the co-dependent transcriptional regulation. In addition, experiments presented in this thesis suggest that phase delays are evoked by the disruption of the PER-TIM complex in the cytoplasm, whereas phase advances are induced by the disruption of the PER-TIM complex in the nucleus.
520 $a In another line of research I sought to understand better how per and tim are regulated at the transcriptional level. In these studies, dCLOCK was characterized and shown to interact with the per and tim proteins in vivo . The dClock mRNA and protein products undergo daily fluctuations in abundance. Furthermore, experiments presented in this thesis suggest that PER, TIM or both inhibit the DNA binding activity of a dCLOCK-CYC heterodimer. Surprisingly, the binding of PER or TIM does not disrupt the dCLOCK-CYC complex indicating the formation of trimeric and higher order structures. In addition, PER and TIM were shown to function as positive regulators. With the recent finding that mammalian and Drosophila clocks have similar components, findings presented in this thesis might add significant insights into numerous human disorders associated with clock malfunction.
590 $a School code: 0801.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
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690 $a 0369
690 $a 0307
710 2 0 $a Rutgers The State University of New Jersey - New Brunswick and University of Medicine and Dentistry of New Jersey. $3 1023857
773 0 $t Dissertation Abstracts International $g 60-01B.
790 1 0 $a Edery, Isaac, $e advisor
790 $a 0801
791 $a Ph.D.
792 $a 1999
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