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Methylation pathway perturbations wi...

Rachlis, Alisa Cheryl.

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Methylation pathway perturbations with folate deficiency: A role for epigenetics in endothelial gene expression.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Methylation pathway perturbations with folate deficiency: A role for epigenetics in endothelial gene expression./
Author:	Rachlis, Alisa Cheryl.
Description:	168 p.
Notes:	Source: Masters Abstracts International, Volume: 42-03, page: 0883.
Contained By:	Masters Abstracts International42-03.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ84383
ISBN:	0612843831

Methylation pathway perturbations with folate deficiency: A role for epigenetics in endothelial gene expression.
Rachlis, Alisa Cheryl.

Methylation pathway perturbations with folate deficiency: A role for epigenetics in endothelial gene expression. - 168 p.

Source: Masters Abstracts International, Volume: 42-03, page: 0883.

Thesis (M.Sc.)--University of Toronto (Canada), 2003.

Folate deficiency is an independent risk factor for atherosclerotic cardiovascular disease. To better understand how folate deficiency elicits changes in vascular wall homeostasis, we established an endothelial cell model of folate deficiency. Increases in steady-state mRNA levels of the folate receptor-alpha and gamma-glutamyl hydrolase and an accumulation of cell-associated homocysteine and SAE were found. Because SAH can inhibit many SAM-mediated methylation reactions, we assessed DNA and histone methylation pathways. Genomic cytosine methylation levels indicated global DNA hypomethylation. In agreement with recent evidence indicating that folate bioavailability affects a key endothelial phenotype, namely the production of NO, we found a concentration-dependent decrease in eNOS steady-state mRNA levels. Decreased eNOS promoter RNA polymerase II loading and dimethylated H3-K4 suggested the folate deficiency can alter gene expression by inhibiting methyltransferase pathways. This work describes, for the first time, a novel mechanism by which folate deficiency can confer a predisposition to cardiovascular pathology.

ISBN: 0612843831Subjects--Topical Terms:

1017719
Biology, Molecular.

Methylation pathway perturbations with folate deficiency: A role for epigenetics in endothelial gene expression.
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100 1 $a Rachlis, Alisa Cheryl. $3 1906009
245 1 0 $a Methylation pathway perturbations with folate deficiency: A role for epigenetics in endothelial gene expression.
300 $a 168 p.
500 $a Source: Masters Abstracts International, Volume: 42-03, page: 0883.
500 $a Adviser: Philip A. Marsden.
502 $a Thesis (M.Sc.)--University of Toronto (Canada), 2003.
520 $a Folate deficiency is an independent risk factor for atherosclerotic cardiovascular disease. To better understand how folate deficiency elicits changes in vascular wall homeostasis, we established an endothelial cell model of folate deficiency. Increases in steady-state mRNA levels of the folate receptor-alpha and gamma-glutamyl hydrolase and an accumulation of cell-associated homocysteine and SAE were found. Because SAH can inhibit many SAM-mediated methylation reactions, we assessed DNA and histone methylation pathways. Genomic cytosine methylation levels indicated global DNA hypomethylation. In agreement with recent evidence indicating that folate bioavailability affects a key endothelial phenotype, namely the production of NO, we found a concentration-dependent decrease in eNOS steady-state mRNA levels. Decreased eNOS promoter RNA polymerase II loading and dimethylated H3-K4 suggested the folate deficiency can alter gene expression by inhibiting methyltransferase pathways. This work describes, for the first time, a novel mechanism by which folate deficiency can confer a predisposition to cardiovascular pathology.
590 $a School code: 0779.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Health Sciences, Nutrition. $3 1017801
690 $a 0307
690 $a 0570
710 2 0 $a University of Toronto (Canada). $3 1017674
773 0 $t Masters Abstracts International $g 42-03.
790 1 0 $a Marsden, Philip A., $e advisor
790 $a 0779
791 $a M.Sc.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ84383