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Application of molecular methods in ...

Thirumalapura, Nagaraja Ramavadhani.

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Application of molecular methods in diagnostics of bacterial pathogens.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Application of molecular methods in diagnostics of bacterial pathogens./
Author:	Thirumalapura, Nagaraja Ramavadhani.
Description:	175 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 0707.
Contained By:	Dissertation Abstracts International66-02B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3167404
ISBN:	054203039X

Application of molecular methods in diagnostics of bacterial pathogens.
Thirumalapura, Nagaraja Ramavadhani.

Application of molecular methods in diagnostics of bacterial pathogens. - 175 p.

Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 0707.

Thesis (Ph.D.)--Oklahoma State University, 2005.

Scope and method of study. The present study investigated the applications of recent advances in molecular techniques for developing novel diagnostics of bacterial pathogens. The study adapted an in vitro selection method known as s&barbelow;ystematic e&barbelow;volution of l&barbelow;igands by exponential enrichment (SELEX) for generation of ssDNA aptamers against Francisella tularensis subspecies tularensis (Strain OSU 10) lipopolysaccharide (LPS). The study also reports the 1H- and 13C-NMR spectroscopy structural analysis of O-antigen of F. tularensis subspecies tularensis (strain OSU 10). Further, microarray based immunoassays were developed for the detection of antibodies to bacterial antigens.

ISBN: 054203039XSubjects--Topical Terms:

1017734
Biology, Microbiology.

Application of molecular methods in diagnostics of bacterial pathogens.
LDR:03165nmm 2200289 4500 001 1816339
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008 130610s2005 eng d
020 $a 054203039X
035 $a (UnM)AAI3167404
035 $a AAI3167404
040 $a UnM $c UnM
100 1 $a Thirumalapura, Nagaraja Ramavadhani. $3 1905727
245 1 0 $a Application of molecular methods in diagnostics of bacterial pathogens.
300 $a 175 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 0707.
500 $a Adviser: Jerry R. Malayer.
502 $a Thesis (Ph.D.)--Oklahoma State University, 2005.
520 $a Scope and method of study. The present study investigated the applications of recent advances in molecular techniques for developing novel diagnostics of bacterial pathogens. The study adapted an in vitro selection method known as s&barbelow;ystematic e&barbelow;volution of l&barbelow;igands by exponential enrichment (SELEX) for generation of ssDNA aptamers against Francisella tularensis subspecies tularensis (Strain OSU 10) lipopolysaccharide (LPS). The study also reports the 1H- and 13C-NMR spectroscopy structural analysis of O-antigen of F. tularensis subspecies tularensis (strain OSU 10). Further, microarray based immunoassays were developed for the detection of antibodies to bacterial antigens.
520 $a Findings and conclusions. Single stranded DNA aptamers were selected against the purified carbohydrate part of LPS (containing core oligosaccharide and O-polysaccharide) from F. tularensis subspecies tularensis (Strain OSU 10). A selected aptamer Ft1 showed specific binding to LPS from F. tularensis subspecies tularensis and subspecies holartica. However, the aptamer did not show significant binding to whole cells of F. tularensis . This could be due to recognition of the core oligosaccharide part of the LPS by the aptamer and the core oligosaccharide may not be readily accessible on the surface of the bacterium. Alternatively, use of whole bacterial cells as target in the selection process could help in developing aptamers to surface targets of bacteria. Structural analysis suggested that the O-antigen of F. tularensis subspecies tularensis strain OSU 10 is identical to that of subspecies holarctica strain 15 and F. tularensis LVS and consists of repeating tetra-saccharide subunits: 4-(alpha-D-GalpNAcAN-(1-4)-alpha-D-GalpNAcAN-(1-3)-beta-D-QuipNAc-(1-2)-beta-D-Quip4NFo-1). A microarray based immunoassay was developed for the detection of antibodies to lipopolysaccharides. The assay showed a hundred-fold greater sensitivity compared to conventional immunoassays. Further, bacterial cell arrays were developed for the detection and characterization of antibodies to cell surface antigens. These microarray-based immunoassays are multiplex in nature and have many potential applications including disease diagnosis and seroepidemiology.
590 $a School code: 0664.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0410
690 $a 0307
710 2 0 $a Oklahoma State University. $3 626630
773 0 $t Dissertation Abstracts International $g 66-02B.
790 1 0 $a Malayer, Jerry R., $e advisor
790 $a 0664
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3167404