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Initial stages of adhesion of Entero...

Senechal, Annie.

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Initial stages of adhesion of Enterococcus faecalis responsible for biofilm formation on medical devices: Quantification of bacterial adhesion and measurement of cell-surface interaction forces.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Initial stages of adhesion of Enterococcus faecalis responsible for biofilm formation on medical devices: Quantification of bacterial adhesion and measurement of cell-surface interaction forces./
Author:	Senechal, Annie.
Description:	86 p.
Notes:	Source: Masters Abstracts International, Volume: 43-04, page: 1380.
Contained By:	Masters Abstracts International43-04.
Subject:	Engineering, Materials Science. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ98565
ISBN:	0612985652

Initial stages of adhesion of Enterococcus faecalis responsible for biofilm formation on medical devices: Quantification of bacterial adhesion and measurement of cell-surface interaction forces.
Senechal, Annie.

Initial stages of adhesion of Enterococcus faecalis responsible for biofilm formation on medical devices: Quantification of bacterial adhesion and measurement of cell-surface interaction forces. - 86 p.

Source: Masters Abstracts International, Volume: 43-04, page: 1380.

Thesis (M.Eng.)--McGill University (Canada), 2004.

The presence of a microbial biofilm on medical implants and devices remains a serious clinical problem since it can increase the risk of infection. To prevent biofilm formation, it is important to study the initial stages of bacterial adhesion. The aim of this study was to compare the initial interactions of the uropathogen Enterococcus faecalis with different medical grade polymers. To accomplish this, kinetics of E. faecalis adhesion were followed using a DNA quantification assay. The influence of biomaterial surface properties, including surface wettability and roughness, was investigated. Moreover, the distribution and morphology of the adhered bacteria were studied. Finally, quantification of bacterial adhesion forces was achieved using AFM through cell detachment measurements.

ISBN: 0612985652Subjects--Topical Terms:

1017759
Engineering, Materials Science.

Initial stages of adhesion of Enterococcus faecalis responsible for biofilm formation on medical devices: Quantification of bacterial adhesion and measurement of cell-surface interaction forces.
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100 1 $a Senechal, Annie. $3 1905510
245 1 0 $a Initial stages of adhesion of Enterococcus faecalis responsible for biofilm formation on medical devices: Quantification of bacterial adhesion and measurement of cell-surface interaction forces.
300 $a 86 p.
500 $a Source: Masters Abstracts International, Volume: 43-04, page: 1380.
500 $a Adviser: Maryam Tabrizian.
502 $a Thesis (M.Eng.)--McGill University (Canada), 2004.
520 $a The presence of a microbial biofilm on medical implants and devices remains a serious clinical problem since it can increase the risk of infection. To prevent biofilm formation, it is important to study the initial stages of bacterial adhesion. The aim of this study was to compare the initial interactions of the uropathogen Enterococcus faecalis with different medical grade polymers. To accomplish this, kinetics of E. faecalis adhesion were followed using a DNA quantification assay. The influence of biomaterial surface properties, including surface wettability and roughness, was investigated. Moreover, the distribution and morphology of the adhered bacteria were studied. Finally, quantification of bacterial adhesion forces was achieved using AFM through cell detachment measurements.
520 $a The results showed significant differences in E. faecalis adhesion between the studied polymers suggesting that precautions should be made for the choice of polymers in the design of medical applications in order to prevent subsequent biofilm formation.
590 $a School code: 0781.
650 4 $a Engineering, Materials Science. $3 1017759
650 4 $a Engineering, Biomedical. $3 1017684
650 4 $a Biology, Microbiology. $3 1017734
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790 1 0 $a Tabrizian, Maryam, $e advisor
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791 $a M.Eng.
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