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The replication of the pheromone dep...

Meloni, Mauro.

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The replication of the pheromone dependent plasmid pCF10 of Enterococcus faecalis and the function of its replication protein PrgW.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The replication of the pheromone dependent plasmid pCF10 of Enterococcus faecalis and the function of its replication protein PrgW./
Author:	Meloni, Mauro.
Description:	144 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-06, Section: B, page: 2944.
Contained By:	Dissertation Abstracts International66-06B.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3178812
ISBN:	054218754X

The replication of the pheromone dependent plasmid pCF10 of Enterococcus faecalis and the function of its replication protein PrgW.
Meloni, Mauro.

The replication of the pheromone dependent plasmid pCF10 of Enterococcus faecalis and the function of its replication protein PrgW. - 144 p.

Source: Dissertation Abstracts International, Volume: 66-06, Section: B, page: 2944.

Thesis (Ph.D.)--Temple University, 2005.

Enterococcus faecalis is a Gram-positive member of the intestinal flora. It can cause serious nosocomial infections such as urinary tract infections, endocarditis and septicemia. The resolution of these infections is often complicated by high levels of antibiotic resistance. In E. faecalis, antibiotic resistance can be associated with pheromone responsive plasmids.

ISBN: 054218754XSubjects--Topical Terms:

1017734
Biology, Microbiology.

The replication of the pheromone dependent plasmid pCF10 of Enterococcus faecalis and the function of its replication protein PrgW.
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035 $a (UnM)AAI3178812
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100 1 $a Meloni, Mauro. $3 1905502
245 1 4 $a The replication of the pheromone dependent plasmid pCF10 of Enterococcus faecalis and the function of its replication protein PrgW.
300 $a 144 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-06, Section: B, page: 2944.
500 $a Chair: Bettina A. Buttaro.
502 $a Thesis (Ph.D.)--Temple University, 2005.
520 $a Enterococcus faecalis is a Gram-positive member of the intestinal flora. It can cause serious nosocomial infections such as urinary tract infections, endocarditis and septicemia. The resolution of these infections is often complicated by high levels of antibiotic resistance. In E. faecalis, antibiotic resistance can be associated with pheromone responsive plasmids.
520 $a The purpose of this study was to characterize the role of the pheromone cCF10 in pCF10 biology. E. faecalis containing pCF10 expresses PrgZ, the cCF10 receptor. cCF10, upon binding to PrgZ, is internalized by the cellular oligopeptide permease, Opp, and interacts with pCF10-encoded molecules that induce aggregation (donor and recipient bacteria) and the transfer of pCF10. After conjugation, E. faecalis continues to produce cCF10 to maintain pCF10.
520 $a To determine why pCF10 maintenance depends on cCF10, pCF10 replication was studied. It was found that the origin of replication of pCF10 is located within prgW. Replication of pCF10 occurs by a unidirectional theta mechanism. PrgW bound to either single-stranded or double-stranded DNA from a direct repeat region within prgW. The replication protein PrgW did not bind to its own promoter.
520 $a Transcription of prgW initiated 1,214 bp upstream of the ATG start codon in a region containing the prgPO operon transcribed on the opposite strand. Replicon stability was affected by regions upstream and downstream of prgW. The prgZY promoter downstream of prgW also disrupted replicon activity. PrgZ increased plasmid stability. PrgW bound the prgZY promoter increasing prgZY transcription.
520 $a cCF10 induces conjugation and maintains pCF10. PrgW binds cCF10. cCF10 does not affect PrgW binding to DNA. In E. faecalis, cCF10 functions in pCF10 replication without inducing conjugation, suggesting these are two separate processes. Lactococcus lactis engineered to produce cCF10 can induce conjugation and maintain pCF10. Streptococcus mutans, able to produce cCF10 active for the induction of conjugation, did not maintain pCF 10 nor regulate prgZY transcription. Conversely, Enterococcus hirae could maintain pCF10, but did not produce cCF10 active for conjugation (V. Mazeffa, unpublished observations). Comparison of these strains may lead to an understanding of how cCF10 affects the biological activity of PrgW.
590 $a School code: 0225.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0410
690 $a 0307
710 2 0 $a Temple University. $3 959342
773 0 $t Dissertation Abstracts International $g 66-06B.
790 1 0 $a Buttaro, Bettina A., $e advisor
790 $a 0225
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3178812