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Characterization of anaerobic benzen...

Ulrich, Anna Christine.

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Characterization of anaerobic benzene-degrading cultures.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of anaerobic benzene-degrading cultures./
Author:	Ulrich, Anna Christine.
Description:	197 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 5331.
Contained By:	Dissertation Abstracts International65-10B.
Subject:	Engineering, Environmental. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ94256
ISBN:	0612942562

Characterization of anaerobic benzene-degrading cultures.
Ulrich, Anna Christine.

Characterization of anaerobic benzene-degrading cultures. - 197 p.

Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 5331.

Thesis (Ph.D.)--University of Toronto (Canada), 2004.

This dissertation describes several studies of anaerobic benzene degradation by microorganisms and fulfills four objectives. The first and second objectives were to characterize microbial populations in two benzene-degrading consortia (one methanogenic and one nitrate-reducing) on a physiological and molecular level. The shortest doubling times, 8 to 9 days, were observed in nitrate-reducing cultures. The highest substrate concentration utilized, 1000 muM, and maximum absolute rates of benzene degraded, 75 muM/day, were observed in methanogenic cultures. Five Bacterial 16S rRNA sequences, one of which resembled a clone previously found in a sulphate-reducing benzene-degrading culture, were identified in the methanogenic culture. Four Bacterial and no Archaeal 16S rRNA sequences were identified in the nitrate-reducing culture. One clone comprised 70% of the culture and was phylogenetically 93% similar to both Azoarcus and Dechloromonas species.

ISBN: 0612942562Subjects--Topical Terms:

783782
Engineering, Environmental.

Characterization of anaerobic benzene-degrading cultures.
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100 1 $a Ulrich, Anna Christine. $3 1905460
245 1 0 $a Characterization of anaerobic benzene-degrading cultures.
300 $a 197 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 5331.
500 $a Adviser: Elizabeth A. Edwards.
502 $a Thesis (Ph.D.)--University of Toronto (Canada), 2004.
520 $a This dissertation describes several studies of anaerobic benzene degradation by microorganisms and fulfills four objectives. The first and second objectives were to characterize microbial populations in two benzene-degrading consortia (one methanogenic and one nitrate-reducing) on a physiological and molecular level. The shortest doubling times, 8 to 9 days, were observed in nitrate-reducing cultures. The highest substrate concentration utilized, 1000 muM, and maximum absolute rates of benzene degraded, 75 muM/day, were observed in methanogenic cultures. Five Bacterial 16S rRNA sequences, one of which resembled a clone previously found in a sulphate-reducing benzene-degrading culture, were identified in the methanogenic culture. Four Bacterial and no Archaeal 16S rRNA sequences were identified in the nitrate-reducing culture. One clone comprised 70% of the culture and was phylogenetically 93% similar to both Azoarcus and Dechloromonas species.
520 $a The third objective was to isolate and characterize pure cultures capable of anaerobic benzene biodegradation. Two co-cultures called Dechloromonas co-culture (DCC) and Azoarcus co-culture (ACC) were successfully isolated from a nitrate-reducing enrichment culture. DCC is &sim;99% Dechloromonas sp. and &sim;1% an unidentified eubacterium clone. ACC is &sim;98% Azoarcus sp. and &sim;2% Pseudomonas sp. Both co-cultures were capable of anaerobic benzene, toluene and benzoate degradation under nitrate-reducing conditions. The isolated Pseudomonas sp. was capable of aerobic benzene degradation and hydrogen utilization under nitrate-reducing conditions. The isolated unidentified eubacterium clone did not exhibit growth on any of the substrates tested. The Dechloromonas and Azoarcus organisms are responsible for anaerobic benzene degradation.
520 $a The fourth objective was to identify the key metabolic steps in the anaerobic benzene degradation pathways. 13C6-benzene experiments were conducted with the methanogenic and nitrate-reducing enrichments. Under methanogenic conditions a maximum of 8 muM 13C6-phenol and 3 muM 13C6-benzoate were detected. These results indicate that under methanogenic conditions a pathway where benzene undergoes hydroxylation to phenol and further transformation to benzoate exists. Under nitratereducing conditions a maximum of 90 nM 13C6-toluene, 120 nM 13C6-benzoate and no phenol were detected as intermediates of benzene degradation. This is the first evidence for a pathway where benzene undergoes methylation to toluene and further transformation to benzoate.
590 $a School code: 0779.
650 4 $a Engineering, Environmental. $3 783782
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0775
690 $a 0410
710 2 0 $a University of Toronto (Canada). $3 1017674
773 0 $t Dissertation Abstracts International $g 65-10B.
790 1 0 $a Edwards, Elizabeth A., $e advisor
790 $a 0779
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ94256